Veterinary Research Communications

, Volume 31, Issue 5, pp 553–558 | Cite as

Computerized Cytomorphometric Analysis of Nuclear Area, Nuclear Perimeter and Mean Nuclear Diameter in Spontaneous Canine Mammary Gland Tumours

Article
First Online:
Accepted:

Abstract

Fifty-two spontaneous canine mammary gland tumours (fibroadenomas (n = 8), tubulopapillary carcinomas (n = 9), solid carcinomas (n = 6), anaplastic carcinomas (n = 7), fibrosarcomas (n = 9), liposarcomas (n = 9) and osteosarcomas (n = 4) were analysed by computer-assisted nuclear morphometry in Hemacolor-stained cytological specimens. Computerized cytomorphometry was performed and the nuclear area, nuclear perimeter and mean nuclear diameter of investigated tumours were assessed. A minimum of 100 nuclei per lesion were examined. The statistical analysis revealed statistically significant differences between benign and malignant neoplasms. The results indicated that computer-assisted nuclear morphometry could be used as an additional method for differentiation of benign from malignant canine mammary gland tumours in cytological specimens.

Keywords

cytology computer-assisted morphometry canine mammary gland tumours 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chieco, P., 2001 Image Cytometry, (BIOS Scientific Publishers Limited, Oxford)Google Scholar
  2. Ciurea, D., Wilkins, R., Shalev, M., Liu, Z., Barba, J. and Gil, G., 1992. Use of computerized interactive morphometry in the diagnosis of mammary adenoma and adenocarcinoma in dogs. American Journal of Veterinary Research, 53, 300–303PubMedGoogle Scholar
  3. Destexhe, E., Vanmanshoven, P. and Coignoul, F., 1995. Comparison of argyrophilic nucleolar organizer regions by counting and image analysis in canine mammary tumours. American Journal of Veterinary Research, 56, 185–187PubMedGoogle Scholar
  4. De Vico, G. and Maiolino, P., 1997. Prognostic value of nuclear morphometry in feline mammary carcinomas. Journal of Comparative Pathology, 117, 99–105PubMedCrossRefGoogle Scholar
  5. De Vico, G., Sfacteria, A., Maiolino, P. and Mazzullo, G., 2002. Comparison of nuclear morphometric parameters on cytologic smears and histologic sections of spontaneous canine tumors. Veterinary Clinical Pathology, 1, 16–18Google Scholar
  6. Griffey, S., Verstraete, F., Kraegel, S., Lucroy, M. and Madewell, B., 1998. Computer-assisted image analysis of intratumoural vessel density in mammary tumors from dogs. American Journal of Veterinary Research, 59, 1238–1242PubMedGoogle Scholar
  7. Harmelin, A., Zuckerman, A. and Nyska, A., 1995. Correlation of Ag-NOR protein measurements with prognosis in canine transmissible venereal tumors. Journal of Comparative Pathology, 112, 429–433PubMedCrossRefGoogle Scholar
  8. Hung, L., Pong, V., Cheng, C., Wong, F. and Chu, R., 2000. An improved system for quantifying AgNOR and PCNA in canine tumors. Anticancer Research, 20, 3273–3280PubMedGoogle Scholar
  9. Juntes, P. and Pogacnik, M., 2000. Morphometric analysis of AgNORs in tubular and papillary parts of canine mammary gland tumors. Analytical and Quantitative Cytology and Histology, 22, 185–192PubMedGoogle Scholar
  10. Kent, M., Griffey, S., Verstraete, F., Naydan, D. and Madewell B., 2002. Computer-assisted image analysis of neovascularization in thyroid neoplasm from dogs. American Journal of Veterinary Research, 63, 363–369PubMedCrossRefGoogle Scholar
  11. Maiolino, P., Papparella, S., Restucci, B. and De Vico, G., 2001. Angiogenesis in squamous cell carcinomas of canine skin: an immunohistochemical and quantitative analysis. Journal of Comparative Pathology, 125, 117–121PubMedCrossRefGoogle Scholar
  12. Maiolino, P., Restucci, B., Papparella, S. and De Vico, G., 2002. Nuclear morphometry in squamous cell carcinomas of canine skin. Journal of Comparative Pathology, 127, 114–117PubMedCrossRefGoogle Scholar
  13. Meijer, G., Belien, J., van Diest, P. and Baak, J., 1997. Image analysis in clinical pathology. Journal of Clinical Pathology, 50, 365–370PubMedGoogle Scholar
  14. Misdorp, B., Else, R., Hellmen, E. and Lipscomb, T., 2001. Histological classification of mammary tumours of the dog and cats. WHO International Histological Classification of Tumours of Domestic Animals, 2nd edn, Vol. VII, (Armed Forces Institute of Pathology, American Registry of Pathology, Washington DC)Google Scholar
  15. Preziozi, R., Della Salda, L., Ricci, A., Simoni, P. and Marcato, P., 1995. Quantification of nuclear organizer regions in canine perineal gland tumours. Research in Veterinary Science, 58, 277–281CrossRefGoogle Scholar
  16. Preziozi, R., Sarli, G. and Paltrinieri, M., 2004. Prognosis value of intratumoural vessel density in cutaneous mast cell tumors of the dog. Journal of Comparative Pathology, 130, 143–154CrossRefGoogle Scholar
  17. Roels, S., Van Daele, A., Van Marck, E. and Ducatelle, R., 2000. DNA ploidy and nuclear morphometric variables for evaluation of melanocytic tumors in dogs and cats. American Journal of, Veterinary Research, 61, 1074–1079CrossRefGoogle Scholar
  18. Strefezzi, F., Xavier, L. and Catao-Dias, J., 2003. Morphometry of canine cutaneous mast cell tumors. Veterinary Pathology, 40, 268–275CrossRefGoogle Scholar
  19. Wandemarin, K., Beletti, M. and Costa, L., 2004. Nuclear morphometry of neoplastic cells as a method for diagnosis of histiocytoma, mastocytoma and transmissible venereal tumours in dogs. Real Time Imaging, 10, 197–204CrossRefGoogle Scholar

Copyright information

© Springer Science + Business Media, Inc. 2007

Authors and Affiliations

  1. 1.Department of General and Clinical Pathology of AnimalsTrakia UniversityStara ZagoraBulgaria
  2. 2.Department of Veterinary Surgery, Faculty of Veterinary MedicineTrakia UniversityStara ZagoraBulgaria

Personalised recommendations