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The Effects of Salivary Gland Extracts from Boophilus microplus Ticks on Mitogen-stimulated Bovine Lymphocytes

  • C. Turni
  • R. P. Lee
  • L. A. Jackson
Article

Abstract

The effect of salivary gland extract (SGE) from the tick Boophilus microplus was examined in mitogen-stimulated lymphocytes in vitro. SGE was added to lymphocytes of seven cattle together with the mitogens concanavalin A (ConA), phytohaemagglutinin (PHA) and pokeweed mitogen (PWM). Semi-purified B cells from another seven cattle were stimulated with the mitogen lipopolysaccharide (LPS). PHA and ConA stimulated proliferation of lymphocytes to the same extent, but the inhibition due to SGE of Boophilus microplus on the proliferative response stimulated by PHA (39.0% ± 9.3%) was less than the inhibition of proliferative response stimulated by ConA (75.4% ± 6.9%). In contrast, SGE of B. microplus stimulated the proliferation of B cells in the presence of LPS in a dose-dependent manner. Enhanced stimulation of B cells by SGE at >4 μg in culture was greater than twice that observed when B cells were stimulated by LPS alone. SGE does not have a direct suppressive effect on bovine B cell proliferation; however, in vivo the effectiveness of B cell responses might be influenced by other immune factors, such as cytokine profiles.

Keywords

Boophilus microplus immunosuppression lymphocytes salivary gland tick 

Abbreviations

BrdU

5-bromo-2′-deoxyuridine

ConA

concanavalin A

LPS

lipopolysaccharide

PHA

phytohaemagglutinin

PWM

pokeweed mitogen

SGE

salivary gland extract

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Copyright information

© Springer Science + Business Media, Inc. 2007

Authors and Affiliations

  1. 1.Department of Microbiology and ParasitologyUniversity of QueenslandSt Lucia
  2. 2.Centre of Infectious Diseases and MicrobiologyWestmead HospitalWestmead
  3. 3.Department of Primary Industries and Fisheries, BiosecurityTick Fever CentreWacolAustralia

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