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Slide Enzyme-Linked Immunosorbent Assay for the Diagnosis of Babesia bigemina Infection in Bovines

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Abstract

A slide enzyme-linked immunosorbent assay (SELISA) for the diagnosis of Babesia bigemina infection in cattle was standardized. Acetone-fixed whole Babesia bigemina-infected erythrocytes on micro-slides were immunoreacted with bovine serum samples followed by antibovine horseradish peroxidase conjugate and developed using diaminobenzidine tetrahydrochloride as a substrate. The positive immunoreactivity (staining pattern) was visualized in the form of dark brown piroplasms. Using the laboratory-standardized SELISA with a sensitivity of 94.4%, the seroprevalence of babesiosis was studied in cattle from two endemic areas of the disease. In comparison to IFAT, SELISA detected higher number of serum samples positive for bovine babesiosis.

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Abbreviations

DAB:

diaminobenzidine tetrahydrochloride

ELISA:

enzyme-linked immunosorbent assay

IFAT:

indirect fluorescent antibody test

PBS:

phosphate-buffered saline

RBC:

red blood corpuscle

SELISA:

slide enzyme-linked immunosorbent assay

References

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Correspondence to R. Ravindran.

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Ravindran, R., Mishra, A.K. & Rao, J.R. Slide Enzyme-Linked Immunosorbent Assay for the Diagnosis of Babesia bigemina Infection in Bovines. Vet Res Commun 31, 999–1004 (2007). https://doi.org/10.1007/s11259-007-0033-4

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Keywords

  • Babesia bigemina
  • slide enzyme-linked immunosorbent assay