Veterinary Research Communications

, Volume 31, Issue 3, pp 343–353 | Cite as

Potential Aflatoxin and Ochratoxin A Production by Aspergillus Species in Poultry Feed Processing

  • M. E. Fraga
  • F. Curvello
  • M. J. Gatti
  • L. R. CavaglieriEmail author
  • A. M. Dalcero
  • C. A. da Rocha Rosa


Poultry feeds are prone to fungal growth and mycotoxin production during processing. The identification of biota with the ability to produce mycotoxins is essential. The aims of this study were (1) to monitor the mycobiota counts at different stages of poultry feed processing; (2) to determine the occurrence of Aspergillus species; (3) to evaluate the natural incidence of aflatoxins and ochratoxin A. The ability of Aspergillus spp. and its teleomorphs isolated here to produce these toxins was also investigated. Samples (144) were collected at random from a factory in Brazil. The occurrence of Aspergillus and Eurotium species was demonstrated on DRBC and DG18 media and the production of aflatoxins and ochratoxin A and their natural incidence were determined by TLC and HPLC methods. A. flavus and E. chevalieri were the most prevalent species isolated. Fungal contamination was not found after the pelleting process, though Aspergillus and Eurotium species were recovered from trough samples. High levels of aflatoxin and ochratoxin A producers were found at all stages of poultry feed processing. Also, high natural contamination with aflatoxins and ochratoxin A was found in the samples. Contact of feed with remainder poultry feed could lead to fungal contamination, so the risk of aflatoxin and/or ochratoxin A contamination of feed must be taken into account.


aflatoxins Aspergillus Eurotium ochratoxin A poultry feed 





dichloran–18% glycerol agar


dichloran–rose bengal–chloramphenicol agar


high-pressure liquid chromatography


ochratoxin A


thin-layer chromatography


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Copyright information

© Springer Science + Business Media, Inc. 2007

Authors and Affiliations

  • M. E. Fraga
    • 1
  • F. Curvello
    • 2
  • M. J. Gatti
    • 3
  • L. R. Cavaglieri
    • 4
    Email author
  • A. M. Dalcero
    • 4
  • C. A. da Rocha Rosa
    • 1
  1. 1.Departamento de Microbiologia e Imunología VeterináriaUniversidade Federal Rural do Rio de Janeiro, Instituto de VeterináriaRio de Janeiro
  2. 2.Departamento de Produção AnimalUniversidade Federal Rural do Rio de Janeiro, Instituto de ZootecniaRio de Janeiro
  3. 3.Departamento de BiologiaFundação Oswaldo Cruz, Instituto Oswaldo CruzRio de JaneiroBrasil
  4. 4.Departamento de Microbiologia e InmunologíaUniversidad Nacional de Rio CuartoRio CuartoArgentina

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