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REP-PCR Analysis of Pasteurella multocida Isolates from Wild and Domestic Animals in India

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Abstract

Repetitive extragenic palindromic sequence-based PCR (REP-PCR) was used to characterize 67 field isolates of Pasteurella multocida originating from different animal species and geographical regions of India. REP-PCR was found to be rapid and reproducible (three repeats were done). These isolates yielded different 23 profiles which were clustered into eight groups. The discrimination index was moderate (D value 0.83). Somatic and antigenic typing of the isolates did not reveal any correlation with REP-PCR profiles. There was no host-specific, type-specific, region-specific or pathenogenicity-specific pattern. The REP profiles of isolates obtained from wild animals were similar to those obtained from domestic animals. Two common bands were present in all the isolates irrespective of somatic or antigenic types. The results were not comparable with earlier findings, which had shown high discrimination index and correlation with disease presentation.

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Abbreviations

cfu:

colony-forming units

DNA:

deoxyribonucleic acid

dNTP:

deoxynucleotide triphosphate

OMP:

outer membrane protein

PFGE:

pulsed-field gel electrophoresis

PM:

Pasteurella multocida

PM-PCR:

Pasteurella multocida-specific polymerase chain reaction

REP:

repetitive extragenic palindromic

References

  1. Amonsin, A., Wellehan J.F., Li L.L., Laber J. and Kapur, V., 2002. DNA fingerprinting of P. multocida recovered from avian sources. Journal of Clinical Microbiology, 40, 3025–31

  2. Biswas, A., Shivchandra, S.B., Saxena, M.K., Kumar, A. Singh, V.P. and Srivastav, S.K., 2004. Molecular variability among the strains of Pasteurella multocida isolated from an outbreak of haemorrhagic septicemia in India. Veterinary Research Communications, 28, 287–298

  3. Blackall, P.J. and Miflin, J.K., 2000. Identification and typing of Pasteurella multocida: a review. Avian Pathology, 29, 271–287

  4. Chen, H.I., Hulten, K. and Clarridge, J.E., 2002. Taxonomic subgroups of P. multocida correlate with clinical presentation. Journal of Clinical Microbiology, 40, 3438–3441

  5. Gunawardana, G.A., Townsend, K.M. and Frost, A.J., 2000. Molecular characterization of avian Pasteurella multocida isolates from Australia and Vietnam by REP-PCR and PFGE. Veterinary Microbiology, 72, 97–109

  6. Hunt, M.L., Alder, B. and Townsend, K.M., 2000. The molecular biology of Pasteurella multocida. Veterinary Microbiology, 72, 3–25

  7. Hunter, R. and Gaston, M.A., 1988. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. Journal of Clinical Microbiology, 26, 2465–2466

  8. Kim, C.J. and Nagaraja, K.V., 1990. DNA fingerprinting for differentiation of field isolates from reference vaccine strains of Pasteurella multocida in turkeys. American Journal of Veterinary Research, 51, 207–210

  9. Mutters, R., Ihm, P., Pohl, S., Frederiksen, W. and Mannheim, W., 1985. Reclassification of genus Pasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis, and Pasteurella langaa. International Journal of Systematic Bacteriology, 35, 309–322

  10. Rimler, R.B. and Glisson, J.R., 1997. Fowl cholera. In: B.W. Calnek (ed), Diseases of Poultry, 10th edn, (Iowa State University Press, Ames, IA), 143–159

  11. Townsend, K.M., Dawkins, H.J. and Papodimitriou, J.M., 1997. Analysis of haemorrhagic septicaemia-causing isolates of Pasteurella multocida by ribotyping and field alternation gel electrophoresis (FAGE). Veterinary Microbiology, 57, 383–395

  12. Van de Peer Y. and De Wachter R., 1997. Construction and evolutionary distance trees with TREECON for Windows: accounting for variation in nucleotide substitution rate among sites. Computer Applications in the Biosciences, 13, 227–230

  13. Verosalovic, J., Kocuth, T. and Lupski, J.R., 1991. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genoms. Nucleic Acids Research, 19, 6823–6831

  14. Wilson, K., 1987. Preparation of genomic DNA from bacteria. In: F.M. Ausabel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl (eds). Current Protocols in Molecular Biology, unit, 2.4.1, (Wiley, New York)

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Author information

Correspondence to B. Sharma.

Additional information

Saxena, M.K., Singh, V.P., Kumar, A.A., Chaudhuri, P., Singh, V.P., Shivachandra, S.B., Biswas, A. and Sharma, B., 2006. REP–PCR analysis of Pasteurella multocida isolates from wild and domestic animals in India. Veterinary Research Communications, 30(8), 851–861

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Saxena, M.K., Singh, V.P., Kumar, A.A. et al. REP-PCR Analysis of Pasteurella multocida Isolates from Wild and Domestic Animals in India. Vet Res Commun 30, 851–861 (2006). https://doi.org/10.1007/s11259-006-3321-5

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Keywords

  • REP-PCR
  • Pasteurella multocida
  • differentiation coefficient
  • dendogram