Repetitive extragenic palindromic sequence-based PCR (REP-PCR) was used to characterize 67 field isolates of Pasteurella multocida originating from different animal species and geographical regions of India. REP-PCR was found to be rapid and reproducible (three repeats were done). These isolates yielded different 23 profiles which were clustered into eight groups. The discrimination index was moderate (D value 0.83). Somatic and antigenic typing of the isolates did not reveal any correlation with REP-PCR profiles. There was no host-specific, type-specific, region-specific or pathenogenicity-specific pattern. The REP profiles of isolates obtained from wild animals were similar to those obtained from domestic animals. Two common bands were present in all the isolates irrespective of somatic or antigenic types. The results were not comparable with earlier findings, which had shown high discrimination index and correlation with disease presentation.
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outer membrane protein
pulsed-field gel electrophoresis
Pasteurella multocida-specific polymerase chain reaction
repetitive extragenic palindromic
Amonsin, A., Wellehan J.F., Li L.L., Laber J. and Kapur, V., 2002. DNA fingerprinting of P. multocida recovered from avian sources. Journal of Clinical Microbiology, 40, 3025–31
Biswas, A., Shivchandra, S.B., Saxena, M.K., Kumar, A. Singh, V.P. and Srivastav, S.K., 2004. Molecular variability among the strains of Pasteurella multocida isolated from an outbreak of haemorrhagic septicemia in India. Veterinary Research Communications, 28, 287–298
Blackall, P.J. and Miflin, J.K., 2000. Identification and typing of Pasteurella multocida: a review. Avian Pathology, 29, 271–287
Chen, H.I., Hulten, K. and Clarridge, J.E., 2002. Taxonomic subgroups of P. multocida correlate with clinical presentation. Journal of Clinical Microbiology, 40, 3438–3441
Gunawardana, G.A., Townsend, K.M. and Frost, A.J., 2000. Molecular characterization of avian Pasteurella multocida isolates from Australia and Vietnam by REP-PCR and PFGE. Veterinary Microbiology, 72, 97–109
Hunt, M.L., Alder, B. and Townsend, K.M., 2000. The molecular biology of Pasteurella multocida. Veterinary Microbiology, 72, 3–25
Hunter, R. and Gaston, M.A., 1988. Numerical index of the discriminatory ability of typing systems: an application of Simpson's index of diversity. Journal of Clinical Microbiology, 26, 2465–2466
Kim, C.J. and Nagaraja, K.V., 1990. DNA fingerprinting for differentiation of field isolates from reference vaccine strains of Pasteurella multocida in turkeys. American Journal of Veterinary Research, 51, 207–210
Mutters, R., Ihm, P., Pohl, S., Frederiksen, W. and Mannheim, W., 1985. Reclassification of genus Pasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis, and Pasteurella langaa. International Journal of Systematic Bacteriology, 35, 309–322
Rimler, R.B. and Glisson, J.R., 1997. Fowl cholera. In: B.W. Calnek (ed), Diseases of Poultry, 10th edn, (Iowa State University Press, Ames, IA), 143–159
Townsend, K.M., Dawkins, H.J. and Papodimitriou, J.M., 1997. Analysis of haemorrhagic septicaemia-causing isolates of Pasteurella multocida by ribotyping and field alternation gel electrophoresis (FAGE). Veterinary Microbiology, 57, 383–395
Van de Peer Y. and De Wachter R., 1997. Construction and evolutionary distance trees with TREECON for Windows: accounting for variation in nucleotide substitution rate among sites. Computer Applications in the Biosciences, 13, 227–230
Verosalovic, J., Kocuth, T. and Lupski, J.R., 1991. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genoms. Nucleic Acids Research, 19, 6823–6831
Wilson, K., 1987. Preparation of genomic DNA from bacteria. In: F.M. Ausabel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith and K. Struhl (eds). Current Protocols in Molecular Biology, unit, 2.4.1, (Wiley, New York)
Saxena, M.K., Singh, V.P., Kumar, A.A., Chaudhuri, P., Singh, V.P., Shivachandra, S.B., Biswas, A. and Sharma, B., 2006. REP–PCR analysis of Pasteurella multocida isolates from wild and domestic animals in India. Veterinary Research Communications, 30(8), 851–861
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Saxena, M.K., Singh, V.P., Kumar, A.A. et al. REP-PCR Analysis of Pasteurella multocida Isolates from Wild and Domestic Animals in India. Vet Res Commun 30, 851–861 (2006). https://doi.org/10.1007/s11259-006-3321-5
- Pasteurella multocida
- differentiation coefficient