Veterinary Research Communications

, Volume 30, Issue 4, pp 357–367 | Cite as

Cellular Immune Responses to 35 kDa Recombinant Antigen of Mycobacterium avium paratuberculosis

Article

Abstract

Mycobacterium avium paratuberculosis is the causative agent of Johne disease, a chronic ulcerative intestinal condition in ruminant animals. Owing to the predominance of cellular response in subclinical forms of the infection, identification of M. a. paratuberculosis antigens eliciting host cell-mediated immune (CMI) reaction is crucial for early control of the disease. A 35 kDa protein of M. a. paratuberculosis was studied for its ability to elicit CMI responses using a mouse model. Lymphoproliferation and IFN-γ response were used to measure the CMI response. Recombinant 35 kDa protein (P35) stimulated proliferation of mouse mononuclear splenocytes sensitized with M. a. paratuberculosis. The P35 elicited increased nitrite production from mononuclear splenocytes from M. a. paratuberculosis-sensitized mice. In addition, RT-PCR-based semiquantitative IFN-γ measurement showed that stimulation with P35 is associated with significant expression of IFN-γ mRNA in M. a. paratuberculosis-sensitized mouse splenocytes. The results indicate that the 35 kDa protein of M. a. paratuberculosis is associated with CMI response in the host.

Keywords

cellular immune response 35 kDa protein Mycobacterium avium paratuberculosis 

Abbreviations:

CMI

cell-mediated immune response

PBS

1 × phosphate buffered saline, pH 7.4

P35

recombinant 35 kDa protein of M. a. paratuberculosis

RT-PCR

reverse transcription–polymerase chain reaction

IFN-γ

interferon gamma

ConA

concanavalin A

IFA

incomplete Freund's adjuvant

MTT

3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide

ST

stimulation index

TNF-α

tumour necrosis factor α

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Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  • S. H. Basagoudanavar
    • 1
    • 2
  • P. P. Goswami
    • 1
  • V. Tiwari
    • 1
  1. 1.National Biotechnology CentreIndian Veterinary Research Institute (IVRI)IzatnagarIndia
  2. 2.Pennsylvania State University College of MedicineHersheyUSA

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