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Short Communication: Comparison of Diagnostic Efficacy of a Monoclonal Antibody-based Competitive ELISA Test with a Similar Commercial Test for the Detection of Antibodies to Peste des Petits Ruminants (PPR) Virus

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Abbreviations

c-ELISA:

competitive ELISA

ELISA:

enzyme-linked immunosorbent assay

HRPO:

horseradish peroxidase

OPD:

orthophenylenediamine

PBS:

phosphate-buffered saline

PI:

percentage inhibition

PPR:

peste des petits ruminants

VNT:

virus neutralization test

References

  1. Anderson, J., McKay, J.A. and Butcher, R.N., 1991. The use of monoclonal antibodies in competition ELISA for detection of antibodies to rinderpest and peste des petits ruminants viruses. In the seromonitoring of rinderpest throughout Africa: Phase I, (International AtomicEnergy Agency, Vienna), 43–53

  2. Anon, 1994. Recommended procedures for disease and serological surveillance as part of the Global Rinderpest Eradication Programme IAEA-TECDOC 747, (International AtomicEnergy Agency, Vienna)

  3. Bandyopadhyay, S.K., 2002. The economic appraisal of a PPR Control Programme in India. Lead paper presented at XIV Annual Conference and National Seminar on Management of Viral Diseases with Emphasis on Global Trade and World Trade Organization Regime, (Indian Virological Society, Indian Veterinary Research Institute, Hebbal, Bangalore, India)

  4. Dhar, P., Sreenivasa, B.P., Barrett, T., Corteyn, M., Singh, R.P. and Bandyopadhyay, S.K., 2002. Recent epidemiology of peste des petits ruminants virus (PPRV). Veterinary Microbiology, 88, 153–159

  5. Jacobson R.H., 1998. Validation of serological assays for diagnosis of infectious diseases. Revue Scientifique et Technique de l'Office International des Epizooties, 17, 469–486

  6. Jeggo, M.H. and Anderson, J., 1992. FAO/IAEA external quality assurance programme for the FAO/IAEA competitive ELISA results in 1992. In the sero-monitoring of rinderpest throughout Africa: Phase II, (International AtomicEnergy Agency, Vienna), 9–21

  7. Sarkar J., Sreenivasa B.P., Singh R.P., Dhar P. and Bandyopadhyay S.K., 2003. Comparative efficacy of various chemical stabilizers on the thermostability of live attenuated peste des petits ruminants (PPR) vaccine. Vaccine, 21, 4728–4735

  8. Shaila, M.S., Shamaki, D., Forsyth, M.A., Diallo, A., Goatley, L., Kitching, R.P. and Barrett, T., 1996. Geographicdistribution of peste des petits ruminants viruses. Virus Research, 43, 149–153

  9. Singh, R.P., Bandyopadhyay S.K., Sreenivasa, B.P. and Dhar, P., 2004a. Production and Characterization of monoclonal antibodies to peste des petits ruminants (PPR) virus. Veterinary Research Communications, 28, 623–639

  10. Singh, R.P., Sreenivasa, B.P., Dhar P., Shah, L.C. and Bandyopadhyay S.K., 2004b. Development of monoclonal antibody based competitive-ELISA for detection and titration of antibodies to peste des petits ruminants (PPR) virus. Veterinary Microbiology, 98, 3–15

  11. Singh R.P., Sreenivasa B.P., Dhar P. and Bandyopadhyay S.K., 2004c. A sandwich-ELISA for the diagnosis of peste des petits ruminants (PPR) infection in small ruminants using anti-nucleocapsid protein monoclonal antibody. Archives of Virology, 149, 2155–2170

  12. Singh, R.P., Saravanan, P., Sreenivasa, B.P., Singh, R.K. and Bandyopadhyay S.K., 2004d. Prevalence and distribution of peste des petits ruminants (PPR) virus infection in small ruminants of India. Revue Scientifique et Technique de l'Office International des Epizooties (In press)

  13. Singh, R.P., Sreenivasa, B.P., Dhar, P., Roy, R.N. and Bandyopadhyay, S.K., 2000. Development and evaluation of a monoclonal antibody based competitive enzyme-linked immunosorbent assay for the detection of the rinderpest virus antibodies. Revue Scientifique et Technique de l'Office International des Epizooties, 19, 754–763

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Correspondence to R. P. Singh.

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Singh, R.P., Saravanan, P., Sreenivasa, B.P. et al. Short Communication: Comparison of Diagnostic Efficacy of a Monoclonal Antibody-based Competitive ELISA Test with a Similar Commercial Test for the Detection of Antibodies to Peste des Petits Ruminants (PPR) Virus. Vet Res Commun 30, 325–330 (2006). https://doi.org/10.1007/s11259-006-3192-9

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Keywords

  • antibody detection
  • competitive ELISA
  • monoclonal antibody
  • peste des petits ruminants