Veterinary Research Communications

, Volume 30, Issue 1, pp 103–108 | Cite as

Marmoset B95a Cells: A Sensitive System for Cultivation of Peste des Petits Ruminants (PPR) Virus

  • B. P. Sreenivasa
  • R. P. Singh
  • B. Mondal
  • P. Dhar
  • S. K. Bandyopadhyay
Short Communication


B95a cells multistep growth curve peste des petits ruminants virus Vero cells 



cytopathic effect


canine distemper virus


multiplicity of infection


measles virus


peste des petits ruminants


peste des petits ruminants virus


rinderpest virus


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  1. Dhar, P., Sreenivasa, B.P., Barrett, T., Corteyn, M., Singh, R.P. and Bandyopadhyay, S.K., 2002. Recent epidemiology of peste des petits ruminants virus (PPRV). Veterinary Microbiology, 88, 153–159CrossRefPubMedGoogle Scholar
  2. Diallo, A., Taylor, W.P., Lefevre, P.C. and Provost, A., 1989. Attenuation d'une souche de la peste des petits ruminants: candidat pour un vaccin homologue vivant. Revue d'Elevage et de Médicine Vétérinaire des Pays Tropicaux, 42, 311–319Google Scholar
  3. Diallo, A., 2003. Peste des petits ruminants. Featured article published in the website: (Accessed 12 August, 2004)
  4. Ishii, H., Yoshikawa, Y. and Yamanouchi, K., 1986. Adaptation of lapinized rinderpest virus to in vitro growth and attenuation of its virulence in rabbits. Journal General Virology, 67, 275–280Google Scholar
  5. Kai, C., Ochikubo, F., Okita, M., Iinuma, T., Mikami, T., Kobune, F. and Yamanouchi, K., 1993. Use of B95a cells for isolation of canine distemper virus from clinical cases. Journal of Veterinary Medical Science, 55, 1067–1070PubMedGoogle Scholar
  6. Kobune, F., Sakata, H. and Sugiura, A., 1990. Marmoset lymphoblastoid cells as a sensitive host for isolation of measles virus. Journal of Virology, 64, 700–705PubMedGoogle Scholar
  7. Kobune, F., Sakata, H., Sugiyama, M. and Sugiura, A., 1994. B95a, a marmoset lymphoblastoid cell line, as a sensitive host for rinderpest virus. Journal of General Virology, 72, 687–692Google Scholar
  8. Lund, B.T. and Barrett, T., 2000. Rinderpest virus infection in primary bovine skin fibroblasts. Archives of Virology, 145, 1231–1237CrossRefPubMedGoogle Scholar
  9. Miller, G., Shope, T., Lisco, H. and Lipman, M., 1972. Epstein–Barr virus: transformation, cytopathic changes, and viral antigens in squirrel monkey and marmoset leukocytes. Proceedings of the National Academy of Sciences of the USA, 69, 383–389Google Scholar
  10. Murphy, F.A., Gibbs, E.P.J., Horzinek, M.C. and Studdert, M.J., 1999. Veterinary Virology, (Academic Press, San Diego)Google Scholar
  11. Reed, L.J. and Muench, H.A., 1938. Simple method of estimating fifty per cent end point. American Journal of Hygiene, 27, 493Google Scholar
  12. Robertson, J.S., Bootman, J.S., Newman, R., Oxford, J.S., Daniels, R.S., Webster, R.G. and Schild, G.C., 1987. Structural changes in the haemagglutinin which accompany egg adaptation of an influenza A (H1N1) virus. Virology, 160, 31–37CrossRefPubMedGoogle Scholar
  13. Sarkar, J., Sreenivasa, B.P., Singh, R.P., Dhar, P. and Bandyopadhyay, S.K., 2004. Comparative efficacy of various chemical stabilizers on the thermostability of a live-attenuated peste des petits ruminants (PPR) vaccine. Vaccine, 21, 4728–4735Google Scholar
  14. Schild, G.C., Oxford, J.S., de Jong, J.C. and Webster, R.G., 1983. Evidence for host-cell selection of influenza virus antigenicvariants. Nature, 303, 706–709CrossRefPubMedGoogle Scholar
  15. Seki, F., Ono, N., Yamaguchi, R. and Yanagi, Y., 2003. Efficient isolation of wild strains of canine distemper virus in Vero cells expressing canine SLAM (CD150) and their adaptability to marmoset B95a cells. Journal of Virology, 77, 9943–9950CrossRefPubMedGoogle Scholar
  16. Shishido, A., Yamanouchi, K., Hikita, M., Sato, T., Fukuda, A. and Kobune, F., 1976. Development of a cell culture system susceptible to measles, canine distemper and rinderpest viruses. Archiv fürdie Gesamte Virusforschung, 22, 364–380Google Scholar
  17. Singh, R.P., Bandyopadhyay S.K., Sreenivasa, B.P. and Dhar, P., 2004. A sandwich-ELISA for the diagnosis of peste des petits ruminants (PPR) infection in small ruminants using anti-nucleocapsid protein monoclonal antibody. Archives of Virology (in press; published online 30 June, 2004)Google Scholar
  18. Visser, I.K., van Bressem, M.F., Barrett, T. and Osterhaus, A.D.M.E., 1993. Morbillivirus infections in aquatic mammals. Veterinary Record, 24, 169–178Google Scholar

Copyright information

© Springer Science + Business Media, Inc. 2006

Authors and Affiliations

  • B. P. Sreenivasa
    • 1
    • 2
  • R. P. Singh
    • 1
  • B. Mondal
    • 1
  • P. Dhar
    • 1
  • S. K. Bandyopadhyay
    • 1
  1. 1.Division of VirologyIndian Veterinary Research InstituteNainitalIndia
  2. 2.Indian Veterinary Research Institute, HebbalBangaloreIndia

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