Veterinary Research Communications

, Volume 29, Issue 7, pp 553–562 | Cite as

Isolation of Vagococcus salmoninarum in Rainbow Trout, Oncorhynchus mykiss (Walbaum), Broodstocks: Characterization of the Pathogen

  • I. Ruiz-Zarzuela
  • I. de Bias
  • O. Gironés
  • C. Ghittino
  • J. L. MúAzquiz
Article

Abstract

Coldwater ‘streptococcosis’, caused by Vagococcus salmoninarum, is an emerging disease of rainbow trout in the European Union, causing mortality rates up to 50% in broodstock during the spawning period, with water temperature of 10–12°C. A study to determine the presence and role of this bacterium was undertaken using classical bacteriological techniques confirmed with polymerase chain reaction. This is the first report of isolation of V salmoninarum in relation to outbreaks of mortality in a rainbow trout farm devoted exclusively to broodstock rearing in Spain. A total of 10 isolates of V salmoninarum were characterized by their morphological, cultural, physiological, biochemical and enzymatic traits. Some differences were observed in parameters such as growth on MacConkey agar, H2S production, acid production from starch, and some other minor variations. Isolates were sensitive to erythromycin and oxytetracycline tested in vitro, but treatments conducted in the field were ineffective. An attempt at vaccination did not provide encouraging results.

Keywords

antibiotics diagnosis rainbow trout Vagococcus salmoninarum 

Abbreviations:

cfu

colony-forming unit

PCR

polymerase chain reaction

SxT

sulfamethoxazoletrimethoprim

TSI

triple-sugar-iron medium

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Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • I. Ruiz-Zarzuela
    • 1
  • I. de Bias
    • 1
  • O. Gironés
    • 1
  • C. Ghittino
    • 2
  • J. L. MúAzquiz
    • 1
  1. 1.Fish Pathology Laboratory, Veterinary FacultyUniversity of ZaragozaZaragozaSpain
  2. 2.Fish Pathology LaboratoryIZS – State Veterinary InstitutePerugiaItaly

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