Veterinary Research Communications

, Volume 29, Supplement 2, pp 245–247 | Cite as

Ovine Skin Organotypic Cultures Applied to the Ex vivo Study of Orf Virus Infection

  • A. Scagliarini
  • F. Dal Pozzo
  • L. Gallina
  • A. Guercio
  • E. De Clercq
  • R. Snoeck
  • G. Andrei
Article

Keywords

keratinocytes orf virus organotypic cultures 

Abbreviation

EGF

epidermal growth factor

FCS

foetal calf serum

PLKs

primary lamb keratinocytes

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References

  1. Chen, S.S., Revoltella, R.P., Papini, S., Nichelini, M., Fitzgerald, W., Zimmerberg, J. and Margolis, L., 2003. Multineage differentiation of rhesus monkey embryonic stem cells in three-dimensional culture systems. Stem Cells, 21, 281–295Google Scholar
  2. Mercer, A.A., Anthony, J., Robinson, B.G., Fraser, K. and Carpenter, E., 1987. Conservation and variation in orf virus genome. Virology, 157, 13–23CrossRefPubMedGoogle Scholar
  3. Potten, C.S. and Booth C., 2002. Keratinocyte stem cells: A commentary. The Journal of Investigative Dermatology, 119(4), 888–899CrossRefPubMedGoogle Scholar
  4. Snoeck, R., Holy, A., Dewolf-Peeters, C., Van Den Oord, J., De Clercq, E. and Andrei, G., 2002. Antivaccinia activities of acyclic nucleoside phosphonate derivates in epithelial cells and organotypic cultures. Antimicrobial Agents and Chemotherapy, 46, 3356–3361PubMedCentralCrossRefPubMedGoogle Scholar
  5. Toma, J.G., Akhavan, M., Fernandes, K.J.L., Barnabè-Heider, F., Sadikot, A., Kaplan, D.R. and Miller, F.D., 2001. Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nature Cell Biology, 3Google Scholar

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • A. Scagliarini
    • 1
  • F. Dal Pozzo
    • 1
  • L. Gallina
    • 1
  • A. Guercio
    • 2
  • E. De Clercq
    • 3
  • R. Snoeck
    • 3
  • G. Andrei
    • 3
  1. 1.Department of Veterinary Public Health and Animal PathologyAlma Mater StudiorumBolognaItaly
  2. 2.Istituto Zooprofilattico Sperimentale della Sicilia A. MirriPalermoItaly
  3. 3.Rega Institute for Medical ResearchLeuvenBelgium

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