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Plant Ecology

, Volume 213, Issue 10, pp 1555–1569 | Cite as

Negative evidence of local adaptation to the establishment conditions in a perennial herb

  • José L. Garrido
  • Pedro J. Rey
  • Carlos M. Herrera
  • José M. Ramírez
Article

Abstract

The differential adaptation of populations of the same species to their local environmental conditions through divergent selection, known as local adaptation, is a key step in the process of diversification of species. Here, we explore the local adaptation of the perennial mountain herb Helleborus foetidus to variable environmental conditions of seedling emergence and establishment at two different spatial scales (habitats and regions) with special attention to the role of physical and chemical soil properties. The possibility of local adaptation was evaluated under the “local versus foreign” and the “home versus away” criteria. Reciprocal sowing experiments were carried out by cross-sowing seeds among habitats and regions, controlling for maternal effects by means of seed mass, and recording seedling emergence and survival. Several topsoil properties were measured linked to each sowing point. Only partial patterns of local adaptation were found, which were insufficient to eventually state the existence of local adaptation at any spatial scale or under any criteria assessed. Here, we discuss how soil properties and selection on seed size may be related to the non-achievement of local adaptation criteria. Negative evidence of local adaptation seems to be due to a congruency in the selective pressures exerted by the different soil environments on seedling emergence and survival.

Keywords

Local adaptation Helleborus foetidus Topsoil properties Seedling emergence Seedling establishment Divergent selection 

Notes

Acknowledgments

The authors thank Jorge Garrido and Ester Orive for their invaluable field assistance. The experiments performed in this work comply with all current Spanish law on investigation matters. We also thank the Junta Rectora of the Natural Parks of “Sierra de Cazorla, Segura y Las Villas” and “Sierra Mágina” for providing working facilities. This study was supported by grant PB96-0856 (DGES: Ministerio de Educación y Cultura, Spain).

Supplementary material

11258_2012_111_MOESM1_ESM.pdf (51 kb)
Appendix 1. Relationship between water availability and water retention capacity across the four habitats considered. Contrarily to the rest of habitats, at Dense Oakwood soils, water availability increased with retention capacity. (PDF 50 kb)

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • José L. Garrido
    • 1
  • Pedro J. Rey
    • 2
  • Carlos M. Herrera
    • 1
  • José M. Ramírez
    • 2
  1. 1.Estación Biológica de DoñanaConsejo Superior de Investigaciones Científicas (CSIC)SevillaSpain
  2. 2.Departamento de Biología Animal, Biología Vegetal y EcologíaUniversidad de JaénJaénSpain

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