Plant Ecology

, Volume 210, Issue 1, pp 31–41 | Cite as

Regeneration of Robinia pseudoacacia riparian forests after clear-cutting along the Chikumagawa River in Japan

  • Hiroyuki KurokochiEmail author
  • Keisuke Toyama
  • Taizo Hogetsu


Regeneration processes of riparian Robinia pseudoacacia forests after clear-cutting were investigated through dendroecological and microsatellite polymorphism analyses. Age determination of regenerated R. pseudoacacia trees based on the dendroecological analysis revealed that forests regenerating after clear-cutting were composed of trees that mostly established within a few years after clear-cutting. This suggests that the stimulus to form new shoots was evoked by clear-cutting but faded within a few years. Genet identification via the microsatellite polymorphism analysis showed that ramet trees belonging to the same genet were distributed in a cluster. Almost all trees regenerated asexually through new ramet formation on the cut stumps and residual horizontal roots after clear-cutting. AMOVA with microsatellite markers showed that among- and within-population variations contributed 6 and 94% to the total variation, respectively, suggesting that R. pseudoacacia trees in the forests were initially established from seeds dispersed randomly from mother trees in a wide area.


AMOVA Asexual reproduction Dendroecology Regeneration processes Robinia pseudoacacia SSR analysis 



We thank the staff of the Chikumagawa River Office, Ministry of Land, Infrastructure, Transport and Tourism for their invaluable help, including logging the R. pseudoacacia trees in the field, and members of the Laboratory of Forest Botany, the University of Tokyo, for various help and support in both the field and laboratory. This study was supported in part by a Grant-in-Aid for Young Scientists from the JSPS (No. 6167) to HK, and a Grant-in-Aid for Scientific Research (S) from the JSPS to TH.


  1. Abrams MD, Ruffner CM, DeMeo TE (1998) Dendroecology and species co-existence in an old-growth QuercusAcerTilia tatus slope forest in the central Appalachians, USA. For Ecol Manage 106:9–18CrossRefGoogle Scholar
  2. Aoki Y (2002) Statistical and probabilistic bases of forensic DNA testing. J Iwate Med Assoc 54:81–94Google Scholar
  3. Boring LR, Swank WT (1984) The role of black locust (Robinia pseudoacacia) in forest succession. J Ecol 72:749–766CrossRefGoogle Scholar
  4. Boring LR, Monk CD, Swank WT (1981) Early regeneration of a clearcut southern Appalachian forest. Ecology 62:1244–1253CrossRefGoogle Scholar
  5. Copenheaver CA, Abrams MD (2003) Dendroecology in young stands: case studies from jack pine in northern lower Michigan. For Ecol Manage 182:247–257CrossRefGoogle Scholar
  6. Elliott KJ, Boring LR, Swank WT (1998) Changes in vegetation structure and diversity after grass-to-forest succession in a Southern Appalachian watershed. Am Midl Nat 140:219–232CrossRefGoogle Scholar
  7. Gyokusen K, Iijima Y, Yahata H (1991) Spatial distribution and morphological features of root sprouts in niseakashia (Robinia pseudoacacia) growing under a coastal black pine forest. Bull Kyusyu Univ For 54:13–28Google Scholar
  8. Honma H (1981) Tests for killing Robinia pseudoacacia L. trees. Bull Niigata Prefect For Exp Stn 24:23–28Google Scholar
  9. Honma H, Shimizu S (1980) Tests for killing Robinia pseudoacacia L. trees. Jpn Bull Niigata Prefect For Exp Stn 23:35–43Google Scholar
  10. Iliev N, Iliev I, Park Y (2005) Black locust (Robinia pseudoacacia L.) in Bulgaria. J Korean For Soc 94:291–301Google Scholar
  11. Iwai H (1986) Tests for the inhibitions of sprouts and growth of Robinia pseudoacacia L. Jpn Bull Chiba Prefect For Exp Stn 20:31–32Google Scholar
  12. Jung SC, Matsushita N, Wu BY, Kondo N, Shiraishi A, Hogetsu T (2009) Reproduction of a Robinia pseudoacacia population in a coastal Pinus thunbergii windbreak along the Kujukurihama Coast. Japan J For Res 14:101–110Google Scholar
  13. Keim RF, Chambers JL, Hughes MS, Dimov LD, Conner WH, Shaffer GP, Gardiner ES, Day JW (2006) Long-term success of stump sprouts in high-graded baldcypress-water tupelo swamps in the Mississippi delta. For Ecol Manage 234:24–33CrossRefGoogle Scholar
  14. Kubota A (2009) The ecology of Robinia pseudoacacia L. Bun-ichi-sogo-syuppan, Tokyo, Japan, pp 81–95Google Scholar
  15. Lian C, Hogetsu T (2002) Development of microsatellite markers in black locust (Robinia pseudoacacia) using a dual-suppression-PCR technique. Mol Ecol Notes 2:211–213CrossRefGoogle Scholar
  16. Lian C, Hogetsu T (2007) Genetic structure of Robinia pseudoacacia: a case study of Tama river basin. In: Forestry Technology, No. 781. Japan Forest Technology Association, Tokyo, Japan, pp 12–15Google Scholar
  17. Luken JO, Hinton AC, Baker DG (1991) Assessment of frequent cutting as a plant-community management technique in power-line corridors. Environ Manage 15:381–388CrossRefGoogle Scholar
  18. Maekawa M, Nakagoshi N (1997a) Impact of biological invasion of Robinia pseudo-acacia on zonation and species diversity of dune vegetation in Central Japan. Jpn J Ecol 47:131–143Google Scholar
  19. Maekawa M, Nakagoshi N (1997b) Riparian landscape changes over a period of 46 years, on the Azusa River in central Japan. Landsc Urban Plan 37:37–43CrossRefGoogle Scholar
  20. Muller RN (1990) Forest regeneration following harvest in the central hardwood forests of Kentucky: implications for succession. Trans Kentucky Acad Sci 51:36–42Google Scholar
  21. Nakamura J (2009) The Ecology of Robinia pseudoacacia L. Bun ichi-sogo-syuppan, Tokyo, Japan, pp 43–67Google Scholar
  22. Negreros-Castillo P, Hall RB (2000) Sprouting capability of 17 tropical tree species after overstory removal in Quintana Roo. Mexico For Ecol Manage 126:399–403CrossRefGoogle Scholar
  23. O’Hara KL, Stancioiu PT, Spencer MA (2007) Understory stump sprout development under variable canopy density and leaf area in coast redwood. For Ecol Manage 244:76–85CrossRefGoogle Scholar
  24. Piovesan G, Di Filippo A, Alessamdrini A, Biondi F, Schirone B (2005) Structure, dynamics and dendroecology of an old-growth Fagus forest in the Apennines. J Veg Sci 16:13–28Google Scholar
  25. Sakio H (2003) Can an exotic plant, Robinia pseudoacacia L., be removed from riparian ecosystems in Japan? J Jpn For Soc 36:244–247Google Scholar
  26. Schneider S, Roessli D, Excoffier L (2000) Arlequin ver. 2.000: a software for population genetics data analysis. Genetics and Biometry Laboratory, University of Geneva, SwitzerlandGoogle Scholar
  27. Shure DJ, Phillips DL, Bostick PE (2006) Gap size and succession in cutover southern Appalachian forests: an 18 year study of vegetation dynamics. Plant Ecol 185:299–318CrossRefGoogle Scholar
  28. Takahashi A, Koyama H, Takahashi N (2008) Habitat expansion of Robinia pseudoacacia L. and role of seed banks in the Akagawa River basin. J Jpn For Soc 90:1–5CrossRefGoogle Scholar
  29. Tanaka N, Yagisawa J (2009) Effects of tree characteristics and substrate condition on critical breaking moment of trees due to heavy flooding. Landsc Ecol Eng 5:59–70CrossRefGoogle Scholar
  30. Uehara K (1959) Illustrations of trees. Ariake Shobou, Tokyo, JapanGoogle Scholar
  31. Yamada K, Masaka K (2007) Present distribution and historical background of the invasive alien species Robinia pseudoacacia on former coalmine land in Hokkaido. Jpn J Conserv Ecol 12:94–102Google Scholar
  32. Zhou Z, Miwa M, Hogetsu T (1999) Analysis of genetic structure of a Suillus grevillei population in a Larix kaempferi stand by polymorphism of inter-simple sequence repeat (ISSR). New Phytol 144:55–63CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Hiroyuki Kurokochi
    • 1
    Email author
  • Keisuke Toyama
    • 1
  • Taizo Hogetsu
    • 1
  1. 1.Graduate School of Agricultural and Life SciencesThe University of Tokyo TokyoJapan

Personalised recommendations