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Scintigraphic evaluation of remote pre-conditioning protection against unilateral renal ischemia/reperfusion injury in rats: a longitudinal study

  • Zahra SedaghatEmail author
  • Hossein Fatemikia
  • Kaveh Tanha
  • Maria Zahiri
  • Majid Assadi
Nephrology - Original Paper
  • 35 Downloads

Abstract

Purpose

To determine the role of remote perconditioning (RPeC) on renal function and histology in an animal model of unilateral renal ischemia and reperfusion (IR) injury.

Methods

Rats were subjected to 60 min unilateral renal ischemia. RPeC protocol was the application of four cycles of 5 min IR of left femoral artery during renal ischemia. Assessments of histological changes and renal function were made 24 h, 1 week, or 3 weeks later. 99mTc-DMSA scan was performed using a small-animals SPECT system.

Results

24-h reperfusion decreased the 99mTc-DMSA uptake in the left kidney compared to the intact kidney of control animals. RPeC group has higher uptake compared to the IR group. After 1 week and 3 weeks, uptakes were gradually increased in both groups and no differences were observed. Severe morphological changes in the ischemic kidneys of both groups were observed after 24 h which attenuated after 1 week and 3 weeks. Moreover, no differences in creatinine and BUN levels between IR-treated and intact animals were observed.

Conclusion

These data suggest that RPeC exerts a partially transient improvement in the renal function in the first day after reperfusion. However, long-term follow-up study showed no beneficial effects of RPeC. Moreover, noninvasive 99mTc-DMSA scan revealed a suitable tool in the follow-up evaluation of recovery process in the unilateral renal IR injury models.

Keywords

Ischemia/reperfusion injury Acute kidney injury Recovery process Animal SPECT Scintigraphy Remote ischemic perconditioning Follow-up study Unilateral ischemia Histopathology 

Notes

Acknowledgements

This work was supported by a grant (number 3160) from Bushehr University of Medical Sciences to Sedaghat Z.

Compliance with ethical standards

Conflict of interest

All authors have no conflict of interest.

Ethical approval

All procedures of this study were approved by the Animal Ethic Committee at the Bushehr University of Medical Sciences (ethic code: BPUMS.REC.1394.14) which were in accordance with the Guide for the Care and Use of Laboratory Animals (Eighth Edition, 2011, published by The National Academies Press).

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Zahra Sedaghat
    • 1
    Email author
  • Hossein Fatemikia
    • 1
    • 2
  • Kaveh Tanha
    • 3
    • 5
  • Maria Zahiri
    • 4
  • Majid Assadi
    • 5
  1. 1.Physiology Department, School of MedicineBushehr University of Medical SciencesBushehrIran
  2. 2.Physiology Department, School of MedicineShiraz University of Medical SciencesShirazIran
  3. 3.Medical Physics and Biomedical Engineering DepartmentTehran University of Medical SciencesTehranIran
  4. 4.Department of Anatomical Sciences, School of MedicineBushehr University of Medical SciencesBushehrIran
  5. 5.The Persian Gulf Nuclear Medicine Research CenterBushehr University of Medical SciencesBushehrIran

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