Losartan accelerates the repair process of renal fibrosis in UUO mouse after the surgical recanalization by upregulating the expression of Tregs

  • Jie Song
  • Yangyang Xia
  • Xiang Yan
  • Jia Luo
  • Chunming Jiang
  • Miao Zhang
  • Guo-Ping Shi
  • Wei ZhuEmail author
Nephrology - Original Paper


Obstructive nephropathy is a common cause for chronic kidney disease. Surgery, which is adopted to promptly relieve the obstruction, is the most important method to save damaged kidneys. However, earlier studies have shown that renal function will continue to deteriorate until the terminal stage after the obstruction’ relief. The aim of this study is to explore the renal fibrosis and investigate the effect of losartan on renal fibrosis after the obstruction’ relief using an improved mouse model of relief for unilateral ureteral obstruction (RUUO). Experiments carried out using C57BL/6 mice (n = 30) were randomly divided into RUUO + Losartan group, RUUO group and sham group. Using an improved mouse RUUO model, this study revealed that the mouse kidney for 3- or 7-day unilateral ureteral obstruction undergoing the RUUO surgery was still in a state of injury and fibrosis, while losartan could effectively ameliorate renal fibrosis by upregulating the expression of CD4 + CD25 + Foxp3 + regulatory T cells (Tregs) in kidney after the surgery of RUUO.


RUUO Mouse model Renal fibrosis Losartan Tregs 



This work was supported by Nanjing Municipal Health and Family Planning Commission and Nanjing Health Youth Talent. We are grateful to the Nanjing Drum Tower Hospital Animal Center.


This work was supported by Nanjing Municipal Health and Family Planning Commission (ZKX17018); Nanjing Health Youth Talent (QRX17045).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Jie Song
    • 1
  • Yangyang Xia
    • 2
  • Xiang Yan
    • 3
    • 4
  • Jia Luo
    • 2
  • Chunming Jiang
    • 2
  • Miao Zhang
    • 1
    • 2
  • Guo-Ping Shi
    • 4
  • Wei Zhu
    • 1
    • 2
    Email author
  1. 1.Department of NephrologyThe Drum Tower Clinical College of Nanjing Medical UniversityNanjingChina
  2. 2.Department of Nephrology, Drum Tower HospitalMedical School of Nanjing UniversityNanjingChina
  3. 3.Department of Urology, Drum Tower HospitalMedical School of Nanjing UniversityNanjingChina
  4. 4.Department of MedicineBrigham and Women’s Hospital and Harvard Medical SchoolBostonUSA

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