Losartan accelerates the repair process of renal fibrosis in UUO mouse after the surgical recanalization by upregulating the expression of Tregs
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Obstructive nephropathy is a common cause for chronic kidney disease. Surgery, which is adopted to promptly relieve the obstruction, is the most important method to save damaged kidneys. However, earlier studies have shown that renal function will continue to deteriorate until the terminal stage after the obstruction’ relief. The aim of this study is to explore the renal fibrosis and investigate the effect of losartan on renal fibrosis after the obstruction’ relief using an improved mouse model of relief for unilateral ureteral obstruction (RUUO). Experiments carried out using C57BL/6 mice (n = 30) were randomly divided into RUUO + Losartan group, RUUO group and sham group. Using an improved mouse RUUO model, this study revealed that the mouse kidney for 3- or 7-day unilateral ureteral obstruction undergoing the RUUO surgery was still in a state of injury and fibrosis, while losartan could effectively ameliorate renal fibrosis by upregulating the expression of CD4 + CD25 + Foxp3 + regulatory T cells (Tregs) in kidney after the surgery of RUUO.
KeywordsRUUO Mouse model Renal fibrosis Losartan Tregs
This work was supported by Nanjing Municipal Health and Family Planning Commission and Nanjing Health Youth Talent. We are grateful to the Nanjing Drum Tower Hospital Animal Center.
This work was supported by Nanjing Municipal Health and Family Planning Commission (ZKX17018); Nanjing Health Youth Talent (QRX17045).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.
- 9.Sakaguchi S, Sakaguchi N, Asano M, Itoh M, Toda M (1995) Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains (CD25) Breakdown of a single mechanism of self-tolerance causes various autoimmune diseases. J Immunol (Baltimore, Md.: 1950) 155(3):1151–1164Google Scholar
- 14.Radovic N, Aralica G, Ljubanovic DG, Jelec V, Kontek M (2014) Effect of unilateral ureteral obstruction and anti-angiotensin II treatment on renal tubule cell apoptosis and interstitial fibrosis in rats. Coll Antropol 38(2):583–588Google Scholar
- 18.Mei Y, Yangyang Z, Shuai L et al (2016) Breviscapine prevents downregulation of renal water and sodium transport proteins in response to unilateral ureteral obstruction. Iran J Basic Med Sci 19(5):573–578Google Scholar