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International Urology and Nephrology

, Volume 51, Issue 2, pp 215–222 | Cite as

Kidney fibrosis induced by various irrigation pressures in mouse models of mild and severe hydronephrosis

  • Xiaobing Yao
  • Fan ChengEmail author
  • Weiming Yu
  • Ting Rao
  • Wei Li
  • Sheng Zhao
  • Xiangjun Zhou
  • Jinzhuo Ning
Urology - Original Paper
  • 155 Downloads

Abstract

Objective

We want to study whether the degree of fibrosis in the mild and severe hydronephrosis is different, and whether the irrigation pressure will affect the fibrosis of the hydronephrosis.

Methods

Animal models of mild and severe hydronephrosis in the left kidney were established: 72 healthy C57BL/6 mice were randomly divided into nine groups (eight in each group). The N group was used as a control group, and 0 mmHg pressure perfusion was given. The M and S groups were used as mild and severe hydronephrosis groups, respectively. The mild and severe hydronephrosis groups were subdivided into eight subgroups, M0–M3 and S0–S3. Among them, groups 0, 1, 2, and 3 were perfused with 0 mmHg, 20 mmHg, 60 mmHg, and 100 mmHg, respectively. We investigated the effects of irrigation pressures on renal fibrosis in mild (group M) and heavy (group S) hydronephrosis by quantitative real-time polymerase chain reaction, Western blot analysis, Masson staining and immunohistochemistry staining in mouse models.

Results

Compared with group N, EMT and ECM deposits were significantly aggravated in both the mild and severe hydronephrosis groups, TGF-β signaling pathway-related molecules significantly changed too. In terms of ECM deposition, S2 and S3 are significantly increased compared to S0.The EMT of M2 and M3 changed significantly compared with M0; the EMT of S1, S2 and S3 changed significantly compared with S0.The molecules related to TGF-β signaling pathway also changed: M0 and S0 changed significantly compared with N; M1, M2 and M3 changed significantly compared with M0; compared with S0, S1, S2 and S3 changed significantly.

Conclusion

Compared with mild hydronephrosis, renal fibrosis in severe hydronephrosis is more severe and its tolerance to perfusion pressure is lower. These changes may be related to the TGF-β signalling pathway.

Keywords

Hydronephrosis Irrigation pressures Renal fibrosis TGF-β1 Smad 

Notes

Acknowledgements

This study was supported by the National Natural Science Foundation of China (Grant No.81870471).

Compliance with ethical standards

Conflict of interest

All authors declare no conflicts of interest.

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Xiaobing Yao
    • 1
  • Fan Cheng
    • 1
    Email author
  • Weiming Yu
    • 1
  • Ting Rao
    • 1
  • Wei Li
    • 2
  • Sheng Zhao
    • 1
  • Xiangjun Zhou
    • 1
  • Jinzhuo Ning
    • 1
  1. 1.Department of UrologyRenmin Hospital of Wuhan UniversityWuhanChina
  2. 2.Department of AnesthesiologyRenmin Hospital of Wuhan UniversityWuhanChina

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