MST3 (mammalian Ste20-like protein kinase 3), a novel gene involved in ion homeostasis and renal regulation of blood pressure in spontaneous hypertensive rats
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Defective renal salt and water excretion, together with increased salt intake, frequently contributes to hypertension. Recent studies indicate that Ste20 family kinases, such as proline-alanine-rich Ste20-related kinase (SPAK) and oxidative stress-response protein 1 (OSR1), are regulators of cell volume, ion transport, and hypertension. The aim of this study was to investigate whether mammalian sterile 20-like protein kinase 3 (MST3), which is also a stress-regulated kinase, is involved in the development of hypertension. MST3 expression was compared in Wistar-Kyoto (WKY) and spontaneously hypertensive rat (SHR) kidneys. MST3 expression was markedly reduced in principal cells of the collecting ducts from the renal inner medulla of SHR. The downregulation of MST3 expression was observed before and after the onset of hypertension in SHR. Mice fed high-salt diets (HS) exhibited a significant increase in MST3 protein level. This is the first study reporting that MST3, a Ste20-like kinase, exerts a conserved regulatory role in sodium homeostasis after high-salt diet and in the development of hypertension.
KeywordsSte20-like kinase MST3 Collecting duct Hypertension High-salt diet
This study was funded by the research Grant HWA-10401012 from the Chung Hwa University of Medical Technology, and in part by the Grants CMFHR10616 from Chi-Mei Medical Center and RD106058 from Chang Bing Show-Chwan Memorial Hospital, Taiwan.
Compliance with ethical standards
Conflict of interest
Author Te-Jung Lu has received research grants from Chung Hwa University of Medical Technology. Author Chee-Hong Chan has received research grants from Chang Bing Show-Chwan Memorial Hospital. Author Wei-Chih Kan has received research grants from Chi-Mei Medical Center. These authors declare that he/she has no conflict of interest.
This study was approved by the Ethics Committee of Animal Experiments, China Medical University, Taichung, Taiwan, and conducted according to their Animal Experiment Guidelines.
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