International Urology and Nephrology

, Volume 50, Issue 6, pp 1181–1188 | Cite as

Determination of NF-κB and RANKL levels in peripheral blood osteoclast precursor cells in chronic kidney disease patients

  • Gülcan Güneş
  • Nil Doğruer ÜnalEmail author
  • Gülçin Eskandari
  • Ahmet Kiykim
  • Özlem Bölgen Çimen
  • Gülhan Temel
  • Mehmet Burak Y. Çimen
Nephrology - Original Paper



Chronic kidney disease (CKD) is a progressive condition characterized by irreversible loss of functional nephron mass due to variety of causes; an inevitable complication of CKD is metabolic bone disease, and this pathology is called as renal osteodystrophy (ROD). In this study, we aimed to determine the levels of serum sRANKL and intracellular NF-κB levels in peripheral blood osteoclast precursor cells in patients with stage 3 CKD.

Materials and methods

Forty-one male patients aged 35–60 with CKD identified as stage 3 according to GFR calculated on the basis of creatinine values and 27 healthy male subjects with age ranging from 40 to 60 as control group were included in this study. Levels of biochemical parameters, vitamin D3, parathyroid hormone, bone mineral density, sRANKL and NF-κB were determined by using photometric, electrochemiluminescence, HPLC, ELISA and flow cytometric methods in control and patient groups, respectively.


When stage 3 CKD patients were compared with controls, patients with stage 3 CKD had statistically significantly higher iPTH levels, but they had statistically significantly lower vitamin D3 levels. However, the other biochemical parameters, bone mineral density, sRANKL and NF-κB levels did not reveal any significance.


In conclusion, vitamin D3 and iPTH levels seem to be important parameters for evaluating the early stages of ROD. The lack of statistically significant differences in the levels of sRANKL and NF-κB suggests that these parameters are not sufficient in the evaluation of bone metabolism in the early stages of renal failure.


Chronic kidney disease NF-κB Renal osteodystrophy sRANKL 



This study was supported by the Scientific Research Projects Unit, Mersin University [BAP-TF (GG) 2009-8 TU].

Compliance with ethical standards

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.


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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiochemistryMersin University Medical FacultyMersinTurkey
  2. 2.Department of BiochemistryMersin University Pharmacy FacultyMersinTurkey
  3. 3.Department of Internal Medicine NephrologyMersin University Medical FacultyMersinTurkey
  4. 4.Department of Physical Therapy and RehabilitationMersin University Medical FacultyMersinTurkey
  5. 5.Department of BiostatisticsMersin University Medical FacultyMersinTurkey

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