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International Urology and Nephrology

, Volume 50, Issue 2, pp 247–255 | Cite as

Anticancer activity of Schiff base–Poloxamer P85 combination against kidney cancer

  • Ayşegül DoğanEmail author
  • Selami Demirci
  • Dilek Telci
  • Serli Canikyan
  • Merve Kongur
  • Bülent Dede
  • Fikrettin Şahin
Urology - Original Paper
  • 262 Downloads

Abstract

Purpose

Renal cell carcinoma (RCC) accounts for approximately 80% of the primary renal cancers, and current treatment strategies are not sufficient to provide a certain solution. Since there are not many treatment options, interest in discovery of alternative drugs has increased.

Methods

In the current study, anticancer activity of a novel heterodinuclear Cu(II)–Mn(II) complex (Schiff base—SB) in combination with poly(ethylene oxide) and poly(propylene oxide) block copolymer (pluronic) P85 was tested against RCC. Cell viability, apoptosis and gene expression analysis were conducted in vitro by using Renca cells.

Results

The results revealed that the SB–P85 combination decreased cell proliferation by increasing the apoptotic gene expressions and apoptosis. Renca-injected BALB/c mice were used to mimic early stage of RCC model. Treatment with SB–P85 combination suppressed tumor formation and growth compared to baseline.

Conclusion

Overall, SB–P85 showed promising anticancer activity against RCC in vitro and in vivo.

Keywords

Schiff base Pluronic P85 Renal cell carcinoma Chemotherapy 

Notes

Acknowledgements

This study was supported by İstanbul Kalkınma Ajansı (ISTKA) Project (ISTKA/2012/BIL-164) and Yeditepe University.

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflict of interest.

Human and animal rights

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed. All experimental procedures were approved by Yeditepe University Ethics Committee of Experimental Animal Use and the Research Scientific Committee (1.4.2013- ISTKA-BIL-164).

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2017

Authors and Affiliations

  • Ayşegül Doğan
    • 1
    • 2
    Email author
  • Selami Demirci
    • 1
    • 3
  • Dilek Telci
    • 1
  • Serli Canikyan
    • 4
  • Merve Kongur
    • 5
  • Bülent Dede
    • 6
  • Fikrettin Şahin
    • 1
  1. 1.Genetics and Bioengineering Department, Faculty of EngineeringYeditepe UniversityKayisdagi, IstanbulTurkey
  2. 2.National Cancer Instıtute, CDBL, NIHFrederickUSA
  3. 3.National Heart, Lung and Blood Institute (NHLBI), NIHBethesdaUSA
  4. 4.Onkim Stem Cell TechnologiesIstanbulTurkey
  5. 5.Acıbadem Labcell, Stem Cell Laboratory and Umbilical Cord Blood BankIstanbulTurkey
  6. 6.Department of Chemistry, Faculty of Sciences and ArtsSüleyman Demirel UniversityIspartaTurkey

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