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Importance of contraction history on muscle force of porcine urinary bladder smooth muscle

Abstract

Introduction

The purpose of this study was to provide a comprehensive dataset of porcine urinary bladder smooth muscle properties. Particularly, the history dependence of force production, namely force depression (FD) following shortening and force enhancement (FE) following stretch, was analysed. During active micturition, the circumference of the urinary bladder changes enormously. Thus, FD might be an important phenomenon during smooth muscle contraction.

Materials and methods

Electrically stimulated, intact urinary bladder strips from pigs (n = 10) were suspended in an aerated-filled organ bath, and different isometric, isotonic, and isokinetic contraction protocols were performed to determine the force–length and the force–velocity relation. FD and FE were assessed in concentric and eccentric contractions with different ramp lengths and ramp velocities.

Results

Bladder smooth muscles exhibit considerable amounts of FD and FE. The amount of FD increased significantly with ramp length, while FE did not change. However, FE and FD were independent of ramp velocity.

Conclusion

The results imply that smooth muscle bladder strips exhibit similar muscle properties and history-dependent behaviour compared to striated muscles. The provided dataset of muscle properties is important for bladder modelling as well as for the analyses and interpretation of dynamic bladder filling and voiding.

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Abbreviations

Curv:

Curvature factor

F im :

Maximum isometric muscle force

FD:

Force depression

FE:

Force enhancement

L opt :

Optimum muscle length associated with F im

L ramp :

Ramp length

L s :

Slack length

L start :

Start length

V max :

Maximal shortening velocity

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Acknowledgements

This work was funded by the Deutsche Forschungsgemeinschaft (DFG) under Grants BO 3091/9-1 and SI 841/9-1.

Author’s contribution

TS and MB conceived and designed the experiments. RM performed the experiments, analysed the data and contributed reagents/materials/analysis tools. RM, MB, and TS wrote the paper.

Author information

Correspondence to Robin Menzel.

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Conflict of interest

The authors declare that there are no conflicts of interest or future conflicts regarding this article.

Ethical approval

All applicable international, national, and institutional guidelines for the care and use of animals were followed.

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Menzel, R., Böl, M. & Siebert, T. Importance of contraction history on muscle force of porcine urinary bladder smooth muscle. Int Urol Nephrol 49, 205–214 (2017). https://doi.org/10.1007/s11255-016-1482-x

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Keywords

  • Force depression
  • Force enhancement
  • Pig
  • Force–length relation
  • Force–velocity relation
  • Muscle properties