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International Urology and Nephrology

, Volume 48, Issue 12, pp 1951–1960 | Cite as

Transplantation of mature adipocyte-derived dedifferentiated fat cells for the treatment of vesicoureteral reflux in a rat model

  • Yuichiro Ikado
  • Daisuke Obinata
  • Taro MatsumotoEmail author
  • Yasutaka Murata
  • Koichiro Kano
  • Noboru Fukuda
  • Kenya Yamaguchi
  • Satoru Takahashi
Urology - Original Paper

Abstract

Purpose

Autologous cells potentially provide an ideal injectable substance for management in vesicoureteral reflux (VUR). The aim of this study is to examine the effects of mature adipocyte-derived dedifferentiated fat (DFAT) cell transplantation on VUR in a rat bladder pressurization-induced VUR model.

Methods

To create VUR, Sprague–Dawley rats underwent urethral clamping and placement of cystostomy followed by intravesical pressurization. Rat DFAT cells (1 × 106 cells, DFAT group, n = 5) or saline (control group, n = 5) was then injected into the bilateral vesicoureteral junctions. Two weeks later, VUR grade was evaluated on cystography. The number of apoptotic cells in the renal pelvic urothelium, the ureteral inner/outer diameter ratio and the area of connective tissue in the posterior bladder wall were measured.

Results

The reflux grade in the DFAT group was significantly lower than that in the control group. The number of apoptotic cells in the renal pelvic urothelium, ureteral inner/outer diameter ratio and connective tissue area in DFAT group were significantly lower in comparison with the control group.

Conclusions

DFAT cell transplantation improved VUR and exerted nephroprotective effects in a rat VUR model.

Keywords

Adipocyte Adult stem cell Cell therapy Mesenchymal stem cell Regenerative medicine 

Notes

Acknowledgments

This research was supported by JSPS KAKENHI Grant Number 26293170, by JSPS Program for Creating Start-ups from Advanced Research and Technology (START Program, ST261006IP) and by MEXT-Supported Program for the Strategic Research Foundation at Private Universities (S1411018).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Ethical approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practical at which the studies were conducted.

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Department of UrologyNihon University School of MedicineTokyoJapan
  2. 2.Division of Cell Regeneration and Transplantation, Department of Functional MorphologyNihon University School of MedicineTokyoJapan
  3. 3.Laboratory of Cell and Tissue Biology, College of Bioresource ScienceNihon UniversityFujisawaJapan
  4. 4.Advanced Medicine and Advanced Research Institute of Sciences and HumanitiesNihon UniversityTokyoJapan

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