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International Urology and Nephrology

, Volume 47, Issue 10, pp 1605–1610 | Cite as

The protective role of curcumin on testicular tissue after hindlimb ischemia reperfusion in rats

  • Mohammad Ashrafzadeh Takhtfooladi
  • Ahmad Asghari
  • Hamed Ashrafzadeh Takhtfooladi
  • Samaneh Shabani
Urology - Original article

Abstract

Purpose

The aim of this study was to investigate the role of curcumin in remote testicular injury caused by hindlimb ischemia reperfusion (IR).

Materials and methods

Forty male Wistar rats were allocated to four groups: sham (G1), sham + curcumin (G2), IR (G3) and IR + curcumin (G4). Curcumin 200 mg/kg was administered intraperitoneally 2 h prior to IR induction. Lower extremities were subjected to IR induced by infrarenal aortic occlusion for 2 h, followed by 6 h of reperfusion. The rats were euthanized and the testes were removed. Glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA), catalase (CAT) and myeloperoxidase (MPO) activities and histopathological damage scores were determined in right testicular tissues. Left testes were used for wet/dry weight ratio measurement.

Results

Activities of SOD and CAT in testicular tissues were significantly decreased by IR, but curcumin pretreatment increased these levels (P < 0.05). MPO activity in testicular tissues in the G3 was significantly higher than in the G4 (P < 0.05). Significantly increased MDA levels in testicular tissues by IR were decreased by curcumin pretreatment (P < 0.05). Testis tissues showed a significant increase in GPx activity compared to the IR group when curcumin was applied. The wet/dry weight ratio of testicular tissues in the G3 was significantly higher than in the other groups (P < 0.05). In addition, specimens from the G3 had a significantly greater histological injury than those from the G4 (P < 0.05). There were no significant differences in tissue MDA, MPO, SOD, CAT and GPx activities, histological changes and wet/dry weight ratio between the G1, G2 and G4.

Conclusions

According to the findings, we conclude that curcumin has preventive effects in the testicular injury induced by hindlimb IR in rats.

Keywords

Curcumin Testis Remote organ Ischemia reperfusion Oxidative stress 

Notes

Compliance with ethical standards

Conflict of interest

MA. Takhtfooladi declares that he has no conflict of interest. A. Asghari declares that he has no conflict of interest. HA. Takhtfooladi declares that he has no conflict of interest. S. Shabani declares that he has no conflict of interest. H. Hajizadeh declares that he has no conflict of interest.

Ethical approval

All applicable international, national and/or institutional guidelines for the care and use of animals were followed.

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Mohammad Ashrafzadeh Takhtfooladi
    • 1
  • Ahmad Asghari
    • 2
  • Hamed Ashrafzadeh Takhtfooladi
    • 3
  • Samaneh Shabani
    • 2
  1. 1.Young Researchers and Elites Club, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Department of Clinical Science, Science and Research BranchIslamic Azad UniversityTehranIran
  3. 3.Department of Pathobiology, Science and Research BranchIslamic Azad UniversityTehranIran

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