International Urology and Nephrology

, Volume 42, Issue 1, pp 181–185 | Cite as

Paricalcitol reduces basal and lipopolysaccharide-induced (LPS) TNF-α and IL-8 production by human peripheral blood mononuclear cells

  • Theodoros Eleftheriadis
  • Georgia Antoniadi
  • Vassilios Liakopoulos
  • Charalambos Kartsios
  • Ioannis Stefanidis
  • Grammati Galaktidou
Nephrology - Original Paper

Abstract

Introduction

Vitamin D and its analogues proved to exert immunomodulatory effects. Paricalcitol is a vitamin D analogue that is safe. It has been used for years in the treatment of secondary hyperparathyroidism in hemodialysis patients and, importantly, it is less calcemic than vitamin D. In this study the immunomodulatory/anti-inflammatory properties of paricalcitol were evaluated in vitro.

Subject and methods

Ten healthy volunteers enrolled into the study. Peripheral blood mononuclear cells (PBMC) at a concentration of 106 cells per well were cultured for 48 h in the presence or not of lipopolysaccharide (LPS) (100 ng/ml) and in the presence or not of paricalcitol (10−8 M). TNF-α and IL-8 were measured in the supernatants by ELISA.

Results

Basal TNF-α concentration (50.3 ± 22 pg/ml) was reduced by paricalcitol (44.1 ± 23.2 pg/ml). LPS increased TNF-α concentration (150.0 ± 81.7 pg/ml), but paricalcitol reduced it (121.1 ± 69.0 pg/ml). The effect of paricalcitol on IL-8 production was more profound. Basal IL-8 concentration (1926 ± 455 pg/ml) was reduced by paricalcitol (1273 ± 472 pg/ml). LPS increased IL-8 concentration (2361 ± 385 pg/ml), but paricalcitol returned it to its basal level (1849 ± 417 pg/ml).

Conclusion

The in vitro inhibition of transforming growth factor alpha and interleukin 8 by paricalcitol confirms the immunomodulatory properties of this vitamin D analogue.

Keywords

Cytokine inhibition Healthy volunteers Immunomodulatory effects Paricalcitol Vitamin D analogues 

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Copyright information

© Springer Science+Business Media, B.V. 2009

Authors and Affiliations

  • Theodoros Eleftheriadis
    • 1
    • 2
  • Georgia Antoniadi
    • 2
  • Vassilios Liakopoulos
    • 3
  • Charalambos Kartsios
    • 1
  • Ioannis Stefanidis
    • 3
  • Grammati Galaktidou
    • 1
  1. 1.Research Institute, Theagenion Anticancer HospitalThessalonikiGreece
  2. 2.Department of NephrologyGeneral Hospital of SerresSerresGreece
  3. 3.Department of NephrologyUniversity Hospital of LarissaLarissaGreece

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