International Urology and Nephrology

, Volume 41, Issue 1, pp 101–108 | Cite as

The effects of molsidomine on hypoxia inducible factor alpha and Sonic hedgehog in testicular ischemia/reperfusion injury in rats

  • Ali Ihsan Dokucu
  • Hulya Ozturk
  • Hayrettin Ozturk
  • Mehmet Cudi Tuncer
  • Fahri Yilmaz
Urology - Original Paper


This study was designed to determine the effect of molsidomine (MO), a precursor of nitric oxide (NO) donor, on hypoxia inducible factor alpha (HIF-1α) and Sonic hedgehog (Shh) levels considered to be involved in the development of testes ischemia/reperfusion (I-R) injury. Torsions were created by rotating ipsilateral testes 720° in a clockwise direction for 6 h and 1-h detorsion of the testis was performed. A sham operation was performed in group 1 (control, n = 7). In group 2 (I-R/Untreated, n = 7), following 6 h of unilateral testicular torsion, 1-h detorsion of the testis was performed. No drug was given. In group 3 (I-R/MO), after performing the same surgical procedure as in group 2, a NO donor MO was given at the starting time of reperfusion. In group 4 (I-R/L-NAME), after performing the same surgical procedure as in group 2, L-NAME was given at the starting time of reperfusion. Testes malondialdehyde (MDA) levels were determined as well as examining the testes histologically. Treatment of rats with MO produced a significant reduction in the levels of MDA and histopathological score compared to testes I-R groups. The Sonic hedgehog (Shh) expression in the basement membrane of the tubuli seminiferi, and sertoli and germinal cells in testicular tissue, were greatly increased in the I-R/MO group compared to groups 1, 2 and 4. Additionally, the HIF-1α expression in the interstitial spaces in testicular tissue were greatly increased in the I-R/MO group. The results suggest that MO has a protective effect against ischemia/reperfusion injury in rat testes and may affect Shh and HIF-1α signaling pathway.


Ischemia/Reperfusion injury Hypoxia inducible factor alpha (HIF-1α) Sonic hedgehog (Shh) Testes 


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Copyright information

© Springer Science+Business Media, B.V. 2008

Authors and Affiliations

  • Ali Ihsan Dokucu
    • 1
  • Hulya Ozturk
    • 2
  • Hayrettin Ozturk
    • 3
  • Mehmet Cudi Tuncer
    • 4
  • Fahri Yilmaz
    • 5
  1. 1.Department of Pediatric SurgerySisli Etfal Training and Research HospitalIstanbulTurkey
  2. 2.Department of Pediatric SurgeryMedical School, Düzce UniversityDuzceTurkey
  3. 3.Department of Pediatric SurgeryMedical School, Abant Izzet Baysal UniversityBoluTurkey
  4. 4.Department of AnatomyMedical School, Dicle UniversityDiyarbakirTurkey
  5. 5.Department of PathologyMedical School, Abant Izzet Baysal UniversityBoluTurkey

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