Urban Ecosystems

, Volume 19, Issue 2, pp 885–898 | Cite as

Assessing the contribution of fallen autumn leaves to airborne fungi in an urban environment

  • Peter J. Irga
  • Margaret D. Burchett
  • Gabe O’Reilly
  • Fraser R. Torpy
Article

Abstract

Many street trees in urban areas are deciduous and drop leaves during autumn. These leaves are a potential growing substrate for fungi, which when aerosolized and inhaled, can lead to allergy along with more serious diseases. This investigation assessed the potential contribution of fallen leaves to the diversity of airborne fungal propagules during autumn. The senescent leaves of five deciduous tree species prevalent in urban environments were subject to a manipulative experiment in which their phyllospheric fungi were aerosolized, cultured and identified. Aerosolized fungi were compared with fungi detected from direct observation of the phyllosphere. Thirty-nine fungal genera were identified across the plant species sampled, of which twenty-eight were present in corresponding air samples. Significant differences were observed amongst the fungal genera growing on the leaves of the different trees, however few differences were found in the composition of fungal spores that were aerosolized. The dominant genera that were aerosolized were: Penicillium, Cladosporium, Alternaria, Chaetomium, Botrytis and Trichothecium. Some of these fungi are known to produce allergy and other symptoms in humans. As these fungal genera have been commonly identified in autumn air samples in other studies, it is likely that the phyllospheric fungi present on deciduating leaves contribute to the aeromycota of urban areas.

Keywords

Airborne fungi Phylloplane Urban ecology Aeromycota Leaf surface fungi Street trees 

References

  1. Agrios GN (2005) Plant diseases caused by Fungi. In: Agrios GN (ed) Plant Pathology, 5th edn. Academic Press, San Diego, pp. 385–614CrossRefGoogle Scholar
  2. Alami I, Mari S, Clérivet A (1998) A glycoprotein from Ceratocystis fimbriata f. sp. platani triggers phytoalexin synthesis in Platanus x acerifolia cell-suspension cultures. Phytochemistry 48:771–776CrossRefGoogle Scholar
  3. Alexopoulos CJ, Mims CW, Blackwell M (1996) Introductory mycology. Wiley and Sons, BrisbaneGoogle Scholar
  4. Avis TJ (2007) Antifungal compounds that target fungal membranes: applications in plant disease control. Can J Plant Pathol 29:323–329CrossRefGoogle Scholar
  5. Bauer H, Schueller E, Weinke G, Berger A, Hitzenberger R, et al. (2008) Significant contributions of fungal spores to the organic carbon and to the aerosol mass balance of the urban atmospheric aerosol. Atmos Environ 42:5542–5549CrossRefGoogle Scholar
  6. Bureau of Meteorology (2014) Climate data online. http://wwwbomgovau/climate/data/ Accessed 16 September 2014.
  7. Brown JKM, Hovmøller MS (2002) Aerial Dispersal of Pathogens on the Global and Continental Scales and Its Impact on Plant Disease. Science 297:537–541CrossRefPubMedGoogle Scholar
  8. City of Sydney (2011) Part B – Tree Species Selection. In: Sweeney K, Julian P, Kairouz A, Johnson, J (eds) Street Tree Master Plan. Sydney, The City of SydneyGoogle Scholar
  9. City of Sydney (2013) The city’s current canopy cover. In: Sweeney K, Lymbery S, Johnson J (eds) Urban Forest Strategy. Sydney, The City of SydneyGoogle Scholar
  10. Cowan MM (1999) Plant Products as Antimicrobial Agents. Clin Microbiol Rev 12:564–582PubMedPubMedCentralGoogle Scholar
  11. Crook B, Burton NC (2010) Indoor moulds, Sick Building Syndrome and building related illness. Fungal Biology Reviews 24:106–113CrossRefGoogle Scholar
  12. De Hoog GS, Guarro J, Gene J, Figueras MJ (2000) Atlas of Clinical Fungi, 2nd edn. Amer Society for Microbiology, Utrecht, The NetherlandsGoogle Scholar
  13. Dejon L, Mohammed H, Du P, Jacob C, Speicher A (2013) Synthesis of chromenoindole derivatives from Robinia pseudoacacia. MedChemComm 4:1580–1583CrossRefGoogle Scholar
  14. Ellis DH, Davis S, Alexiou H, Handke R, Bartley R (2007) Descriptions of Medical Fungi, 2nd edn. Women’s and Children’s Hospital, Adelaide, AustraliaGoogle Scholar
  15. Esser HJ (2002) The revision of Triadica Lour. (Euphorbiaceae). Harv Pap Bot 7:17–21Google Scholar
  16. Fischer R, Kües U (2006) Asexual Sporulation in Mycelial Fungi. In: Kües U, Fischer R (eds) Growth, Differentiation and Sexuality. The Mycota, vol 1. Springer, Berlin Heidelberg, pp 263–292Google Scholar
  17. Gange AC, Dey S, Currie AF, Sutton BC (2007) Site- and species-specific differences in endophyte occurrence in two herbaceous plants. J Ecol 95:614–622CrossRefGoogle Scholar
  18. Gilbert GS, Webb CO (2007) Phylogenetic signal in plant pathogen–host range. Proc Natl Acad Sci 104:4979–4983CrossRefPubMedPubMedCentralGoogle Scholar
  19. Inácio J, Pereira P, Carvalho M, Fonseca Á, Amaral-Collaço MT, et al. (2002) Estimation and Diversity of Phylloplane Mycobiota on Selected Plants in a Mediterranean–Type Ecosystem in Portugal. Microb Ecol 44:344–353CrossRefPubMedGoogle Scholar
  20. Irga PJ, Torpy FR, Burchett MD (2014) Ecological determinants of the aeromycota in urban Sydney. In 10th International Congress of Aerobiology. Campbelltown, AustraliaGoogle Scholar
  21. Irga PJ, Torpy FR (2015) A survey of the aeromycota of Sydney and its correspondence with environmental conditions: grass as a component of urban forestry could be a major determinant. Aerobiologia In Press. doi:10.1007/s10453-015-9388-0
  22. Jumpponen A, Jones KL (2009) Massively parallel 454 sequencing indicates hyperdiverse fungal communities in temperate Quercus macrocarpa phyllosphere. New Phytol 184(2):438–448CrossRefPubMedGoogle Scholar
  23. Kembel SW, Mueller RC (2014) Plant traits and taxonomy drive host associations in tropical phyllosphere fungal communities. Botany 92:303–311CrossRefGoogle Scholar
  24. Klich MA, Pitt JI (1988) A Laboratory Guide to Common Aspergillus Species and their Teleomorphs. CSIRO Publishing, SydneyGoogle Scholar
  25. Larone DH (2002) Medically Important Fungi: A Guide to Identification, (4th edn). American Society for Microbiology Press, Washington D.CGoogle Scholar
  26. Levetin E, Dorsey K (2006) Contribution of leaf surface fungi to the air spora. Aerobiologia 22:3–12CrossRefGoogle Scholar
  27. Mitakakis T, Guest D (2001) A fungal spore calendar for the atmosphere of Melbourne, Australia, for the year 1993. Aerobiologia 17:171–176CrossRefGoogle Scholar
  28. Newbound M, McCarthy MA, Lebel T (2010) Fungi and the urban environment: A review. Landsc Urban Plan 96(3):138–145. doi:10.1016/j.landurbplan.2010.04.005 CrossRefGoogle Scholar
  29. Osono T (2006) Role of phyllosphere fungi of forest trees in the development of decomposer fungal communities and decomposition processes of leaf litter. Can J Microbiol 52:701–716CrossRefPubMedGoogle Scholar
  30. Osono T, Hirose D (2009) Ecology of Endophytic Fungi Associated with Leaf Litter Decomposition. In: Rai M, Bridge PD (eds) Applied Mycology. CABI Publishing, Wallingford, UK, pp 92–109CrossRefGoogle Scholar
  31. Osono T, Mori A (2004) Distribution of phyllosphere fungi within the canopy of giant dogwood. Mycoscience 45:161–168CrossRefGoogle Scholar
  32. Pepeljnjak S, Šegvić M (2003) Occurrence of fungi in air and on plants in vegetation of different climatic regions in Croatia. Aerobiologia 19:11–19CrossRefGoogle Scholar
  33. Pfaller MA, Pappas PG, Wingard JR (2006) Invasive Fungal Pathogens: Current Epidemiological Trends. Clin Infect Dis 43:S3–S14CrossRefGoogle Scholar
  34. Rutherford S, Owen JAK, Simpson RW (1997) Survey of airspora in Brisbane, Queensland, Australia. Grana 36:114–121CrossRefGoogle Scholar
  35. Sabariego S, Díaz de la Guardia C, Alba F (2000) The effect of meteorological factors on the daily variation of airborne fungal spores in Granada (southern Spain). Int J Biometeorol 44:1–5CrossRefPubMedGoogle Scholar
  36. Schulz B, Boyle C (2005) The endophytic continuum. Mycol Res 109:661–686CrossRefPubMedGoogle Scholar
  37. Schulz B, Römmert A-K, Dammann U, Aust H-J, Strack D (1999) The endophyte-host interaction: a balanced antagonism? Mycol Res 103:1275–1283CrossRefGoogle Scholar
  38. Spitaler R, Gurschler S, Ellmerer E, Schubert B, Sgarbossa M, et al. (2009) Flavonoids from Celtis australis (Cannabaceae). Biochem Syst Ecol 37:120–121CrossRefGoogle Scholar
  39. Tham R, Dharmage SC, Taylor PE, Katelaris CH, Vicendese D, et al. (2014) Outdoor fungi and child asthma health service attendances. Pediatr Allergy Immunol 25:429–449CrossRefGoogle Scholar
  40. Torpy F, Irga P, Brennan J, Burchett M (2013) Do indoor plants contribute to the aeromycota in city buildings? Aerobiologia 29:321–331CrossRefGoogle Scholar
  41. Vega FE, Simpkins A, Aime MC, Posada F, Peterson SW, et al. (2010) Fungal endophyte diversity in coffee plants from Colombia, Hawai’i, Mexico and Puerto Rico. Fungal Ecol 3:122–138CrossRefGoogle Scholar
  42. Visagie CM, Houbraken J, Frisvad JC, Hong SB, Klaassen CHW, et al. (2014) Identification and nomenclature of the genus Penicillium. Stud Mycol 78:343–371CrossRefPubMedPubMedCentralGoogle Scholar
  43. Vorholt JA (2012) Microbial life in the phyllosphere. Nat Rev Microbiol 10:828–840CrossRefPubMedGoogle Scholar
  44. Whipps JM, Hand P, Pink D, Bending GD (2008) Phyllosphere microbiology with special reference to diversity and plant genotype. J Appl Microbiol 105:1744–1755CrossRefPubMedGoogle Scholar
  45. Wickremasinghe UK, Heather WA, Griffin DM (1985) Comparative study of methods for the quantification of fungal spores at the leaf surfaces of Populus xeuramericana. Plant Soil 85:447–449CrossRefGoogle Scholar
  46. Yamamoto N, Kimura M, Matsuki H, Yanagisawa Y (2010) Optimization of a real-time PCR assay to quantitate airborne fungi collected on a gelatin filter. J Biosci Bioeng 109:83–88CrossRefPubMedGoogle Scholar
  47. Zak JC (2002) Implications of leaf surface habitat for fungal community structure and function. In: Lindow SE, Hecht-Poinar EI, Elliott VJ (eds) Phyllosphere Microbiology. APS Press, St Paul, USA, pp 299–315Google Scholar

Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Peter J. Irga
    • 1
  • Margaret D. Burchett
    • 1
  • Gabe O’Reilly
    • 1
  • Fraser R. Torpy
    • 1
  1. 1.Plants and Environmental Quality Research Group, School of Life Sciences, Faculty of ScienceUniversity of Technology SydneyBroadwayAustralia

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