Relationship between concentrations of macro and trace elements in serum and follicular, oviductal, and uterine fluids of the dromedary camel (Camelus dromedarius)
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This study aimed at investigating the relationship between concentrations of macro and trace elements in blood serum, and fluids from small and large follicles (SFF and LFF, respectively), oviduct (OF), and uterus (UF) of female dromedary camels. Fluids from small (2–6 mm) and large follicles (7–20 mm), oviduct and uterus, and blood samples were collected from 19 camels. The results indicated that the concentrations of serum Mg, Fe, and Mn were significantly higher than their follicular fluid, OF, and UF concentrations. Levels of Zn, Fe, Cu, Cr, and Mn were significantly higher in SFF than in LFF. Se and Mo concentrations were higher in LFF. Co concentration was lower in serum than in reproductive tract fluids. Cr concentration was higher in UF and OF than in the serum, SFF, and LFF. High Ca concentration was observed for serum and SFF, followed by LFF. The concentration of Na was about 1.18-fold higher in SFF than in serum, OF, and LFF, and approximately 4.1-fold higher in serum than in UF. K was present in higher concentration in SFF than in serum and LFF; however, its concentration was low in UF and OF. In conclusion, this study shows the concentrations of certain elements in small and large follicular, uterine, and oviductal fluids, which may be low or high depending on their function in the development and growth of follicles. This information can support the development of new media for in vitro oocyte maturation and fertilization of female camels.
KeywordsTrace minerals Follicular fluid Female camel Follicles
We would like to thank the DSR and RSSU (Researchers Support and Services Unit) of King Saud University for their technical support.
This study was supported by King Saud University, Deanship of Scientific Research (DSR), Research Group #RG-1438-066.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Anchordoquy, J.P., Anchordoquy, J.M., Sirini M.A., Mattioli G., Picco S.J., and Furnus C.C., 2013. Effect of different manganese concentrations during in vitro maturation of bovine oocytes on DNA integrity of cumulus cells and subsequent embryo development, Reproduction in Domestic Animal, 48(6), 905–11. doi: https://doi.org/10.1111/rda.12184 CrossRefGoogle Scholar
- Anchordoquy, J.P., Anchordoquy, J.M., Sirini, M.A, Testa J.A., Peral-García, P., and Furnus, C.C., 2015. The importance of manganese in the cytoplasmic maturation of cattle oocytes: blastocyst production improvement regardless of cumulus cells presence during in vitro maturation, Zygote, 1, 139–48.Google Scholar
- Bi, C.M., Zhang, Y.L., Liu, F.J., Zhou, T.Z., Yang, Z.J., Gao, S.Y., Wang, S.D., Chen, X.L., Zhai, X.W., Ma, X.G., Jin, L.J. and Wang S., 2013. The effect of molybdenum on the invitro development of mouse preimplantation embryos, Systems Biology in Reproductive Medicine, 59(2), 69–73.PubMedCrossRefGoogle Scholar
- Geshi, M., Takenouchi, N., Yamauchi, N. and Nagai T., 2000. Effects of sodium pyruvate in nonserum maturation medium on maturation, fertilization, and subsequent development of bovine oocytes with or without cumulus cells, Biology of Reproduction, 63, 1730–1734.PubMedCrossRefPubMedCentralGoogle Scholar
- Junior, A.R.P., Tilburg, M.E., Lobo, M.D.P., Monterio-Moeriro, A.C.O., Melo, C.H.S., Souza-Fabjan, J.M.G., Araugo, A.A., Melo, L.M., Teixeria, D.I.A., Moura, A.A. and Freitas, V.J.F., 2018. Proteomic analysis of follicular fluid from tropically adapted goats. Animal Reproduction Journal, 188, 35–44. doi: https://doi.org/10.1016/j.anireprosci.2017.11.005.CrossRefGoogle Scholar
- Kanwar, J.R., Roy, K., Patel, Y., Zhou, S.F., Singh, M.R., Singh, D., Nasir, M., Sehgal, R., Sehgal, A., Singh, R.S., et al., 2015. Multifunctional iron bound lactoferrin and nanomedicinal approaches to enhance its bioactive functions, Molecules 20(6), 9703–9731.PubMedPubMedCentralCrossRefGoogle Scholar
- Kenny, D.A., Humpherson, P., Leese, H.J., Mooris, D., Tomos, A., Diskin, M.G., et al., 2002. Effect of elevated systemic concentrations of ammonia and urea on the metabolite and ionic composition of oviductal fluid in cattle, Biology of Reproduction, 66, 1797–804.PubMedCrossRefPubMedCentralGoogle Scholar
- Leroy, J.L., Vanholder, T., Delanghe, J.R., Opsomer, G., Vansoom, A., Bols, P.E.,and de Kruif, A., 2004. Metabolite and ionic composition of follicular fluid from different sized follicles and their relationship to serum concentrations in dairy cows, Animal Reproduction Science, 80, 201–211.PubMedCrossRefGoogle Scholar
- Massányi, P., Trandžík, J., Nad, P., Koréneková, B., Skalická, M., Toman, R., Lukáč, N., Strapák, P., Halo M., and Turčan J., 2003. Concentration of copper, iron, zinc, cadmium, lead, and nickel in boar semen and relation to the spermatozoa quality. Journal of Environmental Science and Health, Part A: Toxic/ Hazardous Substances and Environmental Engineering, 38(11), 2643–2651.CrossRefGoogle Scholar
- Ménézo, Y., Guérin, P., and Elde K., 2015. The oviduct: a neglected organ due for re-assessment in IVF. Mini Review. Reproductive BioMedicine Online (2015).Google Scholar
- Phillipo, M., Humphries, W.R., Atkinson, T., Henderson, G.D., and Gaythwaite, P.H., 1987. The effect of molybdenum and iron on copper status, puberty, fertility and oestrus cycles in cattle. The Journal of Agricultural Science, 109, 326–336.Google Scholar
- Ramos, G.B., Sia, A.J., Callejas, N.A.N., Revilla, C.J.P., Alfonso, N., and Sia, S.G., 2013. Pregestational and gestational maternal selenium – supplement: influence on ethanol – induced dysmorphogenesis in murine postimplantation embryos. Asian journal of experimental biological sciences, 4, 361–368.Google Scholar
- Tibary, A., and Anouassi, A., 1997. Reproductive physiology in the female camelidae. In: Theriogenology in camelidae: anatomy, physiology, pathology and artificial breeding. IAVH II. Rabat, Morocco. 169-241.Google Scholar
- Tuormaa, T. E., 2000. Chromium selenium copper and other trace minerals in health and reproduction. Journal of Molecular Medicine, 15, 145–157.Google Scholar
- Ufer, C., Wang, C.C., 2011. The roles of glutathione peroxidases during embryo development. Frontiers in Molecular Neuroscience, 4, 1–14.Google Scholar
- Zhang, Y.L., Liu, F.J., Chen, X.L., Zhang, Z.Q., Shu, R.Z.,Yu, X.L., Zhai, X.W., Jin, L.J., Ma, X.G., Qi, Q., and Liu, Z.J,. 2013. Dual effects of molybdenum on mouse oocyte quality and ovarian oxidative stress. Systems Biology in Reproductive Medicine, 59(6), 312–318.PubMedCrossRefPubMedCentralGoogle Scholar