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Dietary supplementation with Withania somnifera root powder ameliorates experimentally induced Infectious Bursal Disease in chicken

  • Bhaskar GangulyEmail author
  • Meena Mrigesh
  • Premlata Chauhan
  • Sunil Kumar Rastogi
Regular Articles
  • 21 Downloads

Abstract

Infectious Bursal Disease (IBD) is an economically important, immunosuppressive viral disease of chicken. Withania somnifera, a well-known Indian medicinal plant and functional food, finds extensive ethnomedicinal and ethnoveterinary use in the subcontinent. Root extracts of Withania somnifera have been shown to inhibit IBD virus (IBDV) in vitro. The effect of dietary supplementation with whole root powder of Withania somnifera was studied in chicken experimentally infected with IBDV. Dietary supplementation with the root powder improved erythrocytic indices, biochemical parameters, bursal weight index, and lymphocyte stimulation indices, and reduced histopathological insult in the infected birds. Viral load decreased to less than one-fourth in the birds receiving dietary supplementation with Withania somnifera root powder. It could be concluded that continued supplementation of IBDV-infected chicken with Withania somnifera root powder alleviated virus-induced stress and histological and immunological alterations and reduced viral persistence in the host.

Keywords

Infectious Bursal Disease Indian ginseng Withania somnifera Stress Physiology Immunity 

Notes

Acknowledgments

The authors thank Drs. S. Kumar, A. K. Das, A. K. Upadhyay, S. K. Shukla, S. Vatsya, and M. K. Saxena of G.B.P.U.A. & T., Pantnagar, for facilitating different phases of the study.

Funding information

BG received financial support during this study in the form of a Senior Research Fellowship (Ref No. 20-06/2010(i)EU-IV) from the Council of Scientific and Industrial Research, India.

Compliance with ethical standards

All institutional and national guidelines for the care and use of laboratory animals were followed.

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Abramoff MD, Magelhaes PJ, Ram SJ. 2004. Image Processing with ImageJ. Biophotonics Int, 11:36-42.Google Scholar
  2. Akotkar NS, Sarag AN, Rekhate DH, Dhok AP. 2007. Effect of supplementation of Ashwagandha (Withania somnifera) on performance of broilers. Indian J Poultry Sci, 42:92-94.Google Scholar
  3. Arivuchelvan A, Murugesan S, Mekala P. 2013. Immunomodulatory effect of Withania somnifera in broilers treated with high doses of enrofloxacin. Indian J Drugs Dis, 2:276-279.Google Scholar
  4. Arnold M, Durairaj V, Mundt E, Schulze K, Breunig KD, Behrens SE. 2012. Protective vaccination against infectious bursal disease virus with whole recombinant Kluyveromyces lactis yeast expressing the viral VP2 subunit. PloS One, 7:e42870.CrossRefGoogle Scholar
  5. Bharavi K, Reddy AG, Rao GS, Reddy AR, Rao SVR. 2010. Reversal of Cadmium-induced oxidative stress in chicken by herbal adaptogens Withania somnifera and Ocimum sanctum. Toxicol Int, 17:59-63.CrossRefGoogle Scholar
  6. Biswas P, Sharma RK, Biswas A. 2012. Effect of dietary supplementation of Ashwagandha (Withania somnifera) and ascorbic acid on production performance of broiler birds. Indian J Poultry Sci, 47:403-405.Google Scholar
  7. Chandra S, Narang R, Sreenivas V, Bhatia J, Saluja D, Srivastava K. 2014. Association of Angiotensin II Type 1 Receptor (A1166C) Gene Polymorphism and Its Increased Expression in Essential Hypertension: A Case-Control Study. PLoS One, 9:e101502.CrossRefGoogle Scholar
  8. Chauhan RS. 1998. Measurement of humoral immune response In: Laboratory Manual of Immunopathology. G. B. Pant University of Agriculture and Technology, Pantnagar; 37.Google Scholar
  9. Cheville NF. 1967. Studies on the pathogenesis of Gumboro disease in the bursa of Fabricius, spleen, and thymus of the chicken. American J Pathol, 51:527.Google Scholar
  10. Confer AW, MacWilliams PS. 1982. Correlation of hematological changes and serum and monocyte inhibition with the early suppression of phytohemagglutinin stimulation of lymphocytes in experimental infectious bursal disease. Canadian J Comp Med, 46(2):169.Google Scholar
  11. Erlandsen A, Mikolai J, Murison A, Mukherjee S, Zwickey H. 2012. In vivo immune modulating effects of Ashwagandha (Withania somnifera). BMC Complementary Alt Med, 12(Suppl 1):P44.CrossRefGoogle Scholar
  12. Eterradossi N, Saif YM. 2013. Infectious bursal disease. In: Swayne DE, Glisson JR, McDougald LR, Nolan LK, Suarez DL, Nair V. eds. Diseases of Poultry 13th edn. Wiley Blackwell. pp. 219-246.Google Scholar
  13. Fischer AH, Jacobson KA, Rose J, Zeller R. 2008. Hematoxylin and eosin staining of tissue and cell sections. Cold Spring Harbor Protocols, 2008(5):pdb-prot4986.Google Scholar
  14. Ganguly B, Kumar S, Umapathi V, Ambwani T. 2010. Inhibition of Gumboro virus by Withania somnifera extract. In: 5th Uttarakhand State Science and Technology Congress Compendium of Abstracts, Uttarakhand Council for Science and Technology, Dehradun, pp. 216.Google Scholar
  15. Ganguly B, Kumar N, Ahmad AH, Rastogi SK. 2017. Influence of phytochemical composition on in vitro antioxidant and reducing activities of Indian ginseng [Withania somnifera (L.) Dunal] root extracts. J Ginseng Res, doi:/10.1016/j.jgr.2017.05.002CrossRefGoogle Scholar
  16. Ganguly B, Umapathi V, Rastogi SK. 2018. Nitric oxide induced by Indian ginseng root extract inhibits Infectious Bursal Disease virus in chicken embryo fibroblasts in vitro. J Ani Sci Technol, 60:2.  https://doi.org/10.1186/s40781-017-0156-2 CrossRefGoogle Scholar
  17. Kaneko JJ, Harvey JW, Bruss ML. 1997. Clinical Biochemistry of Domestic Animals. 5th ed. Harcourt Brace and Company, Singapore. pp. 900-901.Google Scholar
  18. Kumar A, Umapathi V, Singh R. 2008. Production of Antiserum. In: Techniques of Immunology and Serology. G. B. Pant University of Agriculture and Technology, Pantnagar; pp. 17-30.Google Scholar
  19. Lumeij JT. 1997. Avian Clinical Biochemistry. In: Kaneko JJ, Harvey JW, Bruss ML. eds. Clinical Biochemistry of Domestic Animals. Harcourt Brace and Company, Singapore. pp. 857-884.CrossRefGoogle Scholar
  20. Muller H, Mundt E, Eterradossi N, Islam MR. 2012. Current status of vaccines against infectious bursal disease. Avian Pathol 41(2):133-139.CrossRefGoogle Scholar
  21. Muskett JC, Hopkins IG, Edwards KP, Thornton DH. 1979. Comparison of two Infectious Bursal Disease vaccine strains: Efficacy and potential hazards in susceptible and maternally immune birds. Vet Rec, 104:332-334.CrossRefGoogle Scholar
  22. Natt MP, Herrick CA. 1952. A new blood diluent for counting the erythrocytes and leucocytes of the chicken. Poultry Sci, 31:735-738.CrossRefGoogle Scholar
  23. Puvadolpirod S, Thaxton JP. 2000. Model of Physiological Stress in Chickens 1. Response Parameters. Poultry Sci, 79:363-369.CrossRefGoogle Scholar
  24. Tiwari R, Chakraborty S, Saminathan M, Dhama K, Singh SV. 2014. Ashwagandha (Withania somnifera): Role in safeguarding health, immunomodulatory effects, combating infections and therapeutic applications: A review. J Biol Sci, 14(2):77-94.CrossRefGoogle Scholar
  25. Weiner MA, Weiner J. 1994. Ashwagandha (India ginseng). In: Herbs that Heal. QuantumBooks, Mill Valley, CA; pp.70-72.Google Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Animal Biotechnology Center, Department of Veterinary Physiology and BiochemistryGovind Ballabh Pant University of Agriculture and TechnologyPantnagarIndia
  2. 2.Present Address: Clinical Research Department, Research and Development DivisionAyurvet LimitedBaddiIndia
  3. 3.Department of Veterinary Anatomy, College of Veterinary and Animal SciencesGovind Ballabh Pant University of Agriculture and TechnologyPantnagarIndia

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