Genetic polymorphisms in part of intron 7 and exon 8 of HSP90AA1 gene and its association with heat tolerance traits in two exotic layer chicken strains
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Chickens, like other vertebrates, react to stress conditions through their cultured cells by expressing heat shock proteins (HSPs). Genetic association of single-nucleotide polymorphisms (SNPs) in HSPs with desirable traits will reveal their importance as potential genetic markers. Blood samples were collected from 50 birds per strain for DNA extraction, polymerase chain reaction amplification and sequencing of the HSP90AA1 gene. SNPs were detected using Codoncode Aligner. Association of each SNP with heat tolerance traits was analysed using generalized linear model procedure of SAS. A total of seven SNPs were detected, four SNPs; A7T, A160T, T223A and C134T were detected in part of intron 7 to exon 8 of HSP90AA1 gene of BRD while three A160T, T223A and C134T were detected in HYL. SNPC134T, a synonymous variant, was detected in exon 8. Only SNPA7T was in Hardy-Weinberg equilibrium (X2 = 0.03) but had no association with the traits measured. Polymorphic information content calculated showed SNPA160T to be moderately polymorphic; other SNPs were lowly polymorphic. Heterozygosity for SNPs-A160T and T223A of BRD showed moderate genetic variation while the other SNPs and those in HYL recorded low genetic variation. The study concluded that the SNPs detected were majorly lowly polymorphic and also the SNP locus A7T in intron 7 of HSP90AA1 of BRD had no genetic association with heat tolerance traits.
KeywordsHSP90AA1 Single-nucleotide polymorphism (SNP) Exon Intron Heat tolerance traits Layers
The World Bank Africa Centre of Excellence in Agricultural Development and Sustainable Environment anchored in the Federal University of Agriculture, Abeokuta. Ogun State, Nigeria, sponsored this project (Sponsor ID No: ACE 023).
Compliance with ethical standards
The experiment was conducted following the code of ethics for animal experimentation with prior approval by the University’s Animal Ethics Committee.
Conflict of interest
The authors declare that they have no conflict of interest.
- Ayo, J.O. Obidi, J.A. and Rekwot, P.I. 2011. Effects of Heat Stress on theWell-Being, Fertility, and Hatchability of Chickens in the Northern Guinea Savannah Zone of Nigeria: A Review. International Scholarly Research Network ISRN Veterinary Science Volume 2011, Article ID 838606, 10 pages doi:10.5402/2011/838606.CrossRefGoogle Scholar
- Botstein D., White R.L., Skolnick M.H., Davies R.W. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphism. America Journal of Human Genetics. 32: 314–331.Google Scholar
- Dominant CZ. 2014. Parent Stock; Common Management Guide, Layers Programmes. Lazne Bohdanec. Czech Republic. 119: 533 41. www.dominant-cz.cz. Accessed 12 June 2015.
- FAOSTAT. 2007. Food and Agricultural Organization statistical databases. CDROM.Google Scholar
- Gupta R.S. 1995. Phylogenetic analysis of the 90 kD heat shock family of protein sequences, an examination of the relationship among animals, plants, fungi species. Mol. Biol. Evol. 12(6):1062–1073. https://doi.org/10.1093/oxfordjournals.molbev.a040281 CrossRefGoogle Scholar
- Hall, T.A. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nuclei Acids Symposium. 41: 95–98.Google Scholar
- Kumar, R., Gupta, I. D., Verma, A., Verma, N. and Vineeth, M. R. 2017. Single nucleotide polymorphisms in Heat Shock Protein (HSP) 90AA1 gene and their association with heat tolerance traits in Sahiwal cows. Indian Journal of Animal Research. 51 (1) 64–69. https://doi.org/10.18805/ijar.7081.CrossRefGoogle Scholar
- Marcos-Carcavilla, A., Mutikainen, M., González, C., Jorge, H., Calvo, J., Kantanen, A., Nurbiy, S., Marzanov-María, D. and Magdalena Serrano, J.B. 2010. A SNP in the HSP90AA1 gene 5′ flanking region is associated with the adaptation to differential thermal conditions in the ovine species. Cell Stress Chaperones. 15: 67–81.CrossRefGoogle Scholar
- Pescatello, L.S. and Roth S.M. (eds.). 2011. Exercise Genomics, Molecular and Translational Medicine, Humana Press. 287. ISBN: 9781607613541.Google Scholar
- Sailo, L. and Gupta, L.D., Archana Verma, Ramendra Das and M. V. Chaudhar, 2015. Association of single nucleotide polymorphism of HSP90ab1 gene with thermotolerance and milk yield in Sahiwal cows. African Journal of Biochemistry Research. 9(8): 99–103, https://doi.org/10.5897/AJBR2015.0837 CrossRefGoogle Scholar
- SAS, 2009. Statistical Analysis System User’s Guide. Statistical Analysis Institute, Inc., Cary, North Carolina.Google Scholar