Abstract
This study investigated the effects of streptozotocin (STZ) and S-allyl-L-cysteine (SAC) on motility, plasma membrane integrity, and mitochondrial activity of the boar sperm. STZ (0, 10, 50, and 100 μM) and SAC (0, 1, 5, 25, and 100 μM) were treated alone and co-treated in the fresh boar semen. The motility, plasma membrane integrity, and mitochondrial activity of sperm were analyzed at 3, 6, and 9 h after incubation. Boar semen was collected using the gloved-hand method from ten crossbred male pigs, and age of experimental ten male pigs is 24~27 months. The sperm plasma membrane integrity was analyzed using Live/Dead sperm kit. Mitochondrial activity was analyzed using rhodamine 123 and PI double-staining method. Additionally, sperm motility was evaluated according to standard method. Sperm motility, plasma membrane integrity, and mitochondrial activity were decreased in an STZ concentration-dependent manner (P < 0.05) and also were decreased by 10 μM STZ in all incubation times (P < 0.05). The motility, plasma membrane integrity, and mitochondrial activity of the sperm were increased at 5 μM SAC treatment, whereas it was decreased at 100 μM treatment. In addition, sperm motility, plasma membrane integrity, and mitochondrial activity were increased when co-treated with 50 μM STZ and 5 μM SAC group at 9 h after incubation (P < 0.05). Based on our results, STZ has a deleterious effect on sperm characteristics, and SAC can protect sperm motility, viability, and function of the sperm exposed to STZ.
Abbreviations
- BTS:
-
Beltsville Thawing Solution
- CAT:
-
catalase
- EDTA:
-
ethylenediaminetetraacetic acid
- GLUT:
-
glucose transporter
- GSH:
-
glutathione
- HNE:
-
4-hydroxy-2-nonenal
- MDA:
-
malondialdehyde
- PI:
-
propidium iodide
- R123:
-
rhodamine 123
- SAC:
-
S-allyl-L-cysteine
- SOD:
-
superoxide dismutase
- STZ:
-
streptozotocin
References
Afifi, M. et al. (2015) Ameliorative Effect of Zinc Oxide Nanoparticles on Antioxidants and Sperm Characteristics in Streptozotocin-Induced Diabetic Rat Testes, BioMed research international, pp. 153573–153573.
Amaral, S. et al. (2006) Effects of hyperglycemia on sperm and testicular cells of Goto-Kakizaki and streptozotocin-treated rat models for diabetes, Theriogenology, 66, pp. 2056–2067.
Atta, M.S. et al. (2017) Thymoquinone Defeats Diabetes-Induced Testicular Damage in Rats Targeting Antioxidant, Inflammatory and Aromatase Expression, International journal of molecular sciences, 18, pp. 919.
Ayeleso, A. et al. (2014) Modulation of antioxidant status in streptozotocin-induced diabetic male wistar rats following intake of red palm oil and/or rooibos, Asian Pacific journal of tropical medicine, 7, pp. 536–544.
Bathgate, R. (2011) Antioxidant Mechanisms and their Benefit on Post-thaw Boar Sperm Quality, Reproduction in domestic animals, 46, pp. 23–25.
Brewer, M. (2011) Natural antioxidants: sources, compounds, mechanisms of action, and potential applications, Comprehensive reviews in food science and food safety, 10, pp. 221–247.
Browne, R. K. et al. (2015) Sperm motility of externally fertilizing fish and amphibians
Chen, F. et al. (2013) Antidiabetic effect of total flavonoids from Sanguis draxonis in type 2 diabetic rats, Journal of ethnopharmacology, 149, pp. 729–736.
Ding, G. L. et al. (2015) The effects of diabetes on male fertility and epigenetic regulation during spermatogenesis, Asian journal of andrology, 17, pp. 948–948.
Guo, H. et al. (2017) Relationships between mitochondrial DNA content, mitochondrial activity, and boar sperm motility, Theriogenology, 87, pp. 276–283.
Guzick, D. et al. (1999) National Cooperative Reproductive Medicine Network. Efficacy of superovulation and intrauterine insemination in the treatment of infertility, The New England journal of medicine, 340, pp. 177–183.
Iciek, M. B. et al. (2012) Effects of Different Garlic-derived Allyl Sulfides on Peroxidative Processes and Anaerobic Sulfur Metabolism in Mouse Liver, Phytotherapy research, 26, pp. 425–431.
Javed, H. et al. (2011) S-allyl cysteine attenuates oxidative stress associated cognitive impairment and neurodegeneration in mouse model of streptozotocin-induced experimental dementia of Alzheimer’s type, Brain research, 1389, pp. 133–142.
Kao, S. H. et al. (2008) Increase of oxidative stress in human sperm with lower motility, Fertility and sterility, 89, pp. 1183–1190.
Khanum, F. et al. (2004) Anticarcinogenic properties of garlic: a review, Critical reviews in food science and nutrition, 44, pp. 479–488.
Kim, S. T. and Moley, K. H. (2008) Paternal effect on embryo quality in diabetic mice is related to poor sperm quality and associated with decreased glucose transporter expression, Reproduction, 136, pp. 313–322.
King, A. J. (2012) The use of animal models in diabetes research, British journal of pharmacology, 166, pp. 877–894.
Kokk, K. et al. ( 2004) Immunohistochemical detection of glucose transporters class I subfamily in the mouse, rat and human testis, Medicina (Kaunas), 40, pp. 156–160.
Kosuge, Y. et al. (2003) S-allyl-L-cysteine selectively protects cultured rat hippocampal neurons from amyloid β-protein-and tunicamycin-induced neuronal death, Neuroscience, 122, pp. 885–895.
Lee, A. S. et al. (2017) Effects of curcumin on sperm motility, viability, mitochondrial activity and plasma membrane integrity in boar semen, Biomedical Science Letters, 23, pp. 406–410.
Lee, Y. S. et al. (2015) Effect of cholesterol-loaded-cyclodextrin on sperm viability and acrosome reaction in boar semen cryopreservation, Animal Reproduction Science, 159, pp. 124–130.
Lin, C. C. et al. (2008) Alleviative effects of s-allyl cysteine and s-ethyl cysteine on MCD diet-induced hepatotoxicity in mice, Food and chemical toxicology, 46, pp. 3401–3406.
Saravanan, G. and Ponmurugan, P. (2011) Ameliorative potential of S-allyl cysteine on oxidative stress in STZ induced diabetic rats, Chemico-biological interactions, 189, pp. 100–106.
Saravanan, G. et al. (2010) Antidiabetic effect of S-allylcysteine: Effect on plasma and tissue glycoproteins in experimental diabetes, Phytomedicine, 17, pp. 1086–1089.
Schnedl, W. J. et al. (1994) STZ transport and cytotoxicity: specific enhancement in GLUT2-expressing cells, Diabetes, 43, pp. 1326–1333.
Storey, B. T. (1997) Biochemistry of the induction and prevention of lipoperoxidative damage in human spermatozoa, Molecular human reproduction, 3, pp. 203–213.
Szkudelski, T. (2001) The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas, Physiological research, 50, pp. 537–546.
Takemura, S. et al. (2014) S-allyl cysteine ameliorates the quality of sperm and provides protection from age-related sperm dysfunction and oxidative stress in rats, Journal of clinical biochemistry and nutrition, 55, pp. 155–161.
Thuwanut, P. et al. (2008) The effect of antioxidants on motility, viability, acrosome integrity and DNA integrity of frozen-thawed epididymal cat spermatozoa, Theriogenology, 70, pp. 233–240.
Wang, S. et al. (2018) Effects of L-glutamine on boar sperm quality during liquid storage at 17°C, Animal Reproduction Science, 191, pp. 76–84.
Tremellen, K. (2008) Oxidative stress and male infertility—a clinical perspective, Human reproduction update, 14, pp. 243–258.
Yang, J. et al. (2013) S-allyl cysteine restores erectile function through inhibition of reactive oxygen species generation in diabetic rats, Andrology, 1, pp. 487–494.
Zheng, S. et al. (2015) Sesamin suppresses STZ induced INS-1 cell apoptosis through inhibition of NF-κB activation and regulation of Bcl-2 family protein expression, European journal of pharmacology, 750, pp. 52–58.
Acknowledgments
This work was supported by 2017 Research Grant from Kangwon National University (no. 520170021).
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SL and B-KY designed the experimental study and drafted the manuscript. S-HL analyzed the results and discussed the manuscript. A-SL performed the sperm plasma membrane integrity, motility, and mitochondrial activity assay. All authors read and approved the final manuscript.
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All procedures involving the use of animal experiments were approved by the Kangwon National University Institutional Animal Care and Use Committee (KIACUC-09-0139).
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Lee, AS., Lee, SH., Lee, S. et al. Effects of streptozotocin and S-allyl-L-cysteine on motility, plasma membrane integrity, and mitochondrial activity of boar spermatozoa. Trop Anim Health Prod 52, 437–444 (2020). https://doi.org/10.1007/s11250-019-01983-2
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DOI: https://doi.org/10.1007/s11250-019-01983-2