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Evaluation of a fusion gene-based DNA prime-protein boost vaccination strategy against Newcastle disease virus

  • Sagar A. Khulape
  • Hemanta Kumar Maity
  • Dinesh Chandra Pathak
  • Narayan Ramamurthy
  • Saravanan Ramakrishnan
  • Madhan Mohan ChellappaEmail author
  • Sohini DeyEmail author
Regular Articles
  • 61 Downloads

Abstract

The low potency of genetic immunization has to date impeded development of commercial vaccines against major infectious diseases. The aim of this study was to develop and evaluate a fusion gene-based DNA prime-protein boost vaccination strategy to improve the efficacy of both DNA and subunit vaccines against Newcastle disease virus (NDV). The fusion (F) protein, a viral surface glycoprotein, is responsible for the cell membrane fusion and spread, also is one of the major targets for immune response. In this study, groups of chickens were vaccinated twice intramuscularly at 14-day interval either with plasmid DNA encoding F protein gene of NDV or with recombinant F protein alone or with plasmid DNA and boosted with the recombinant F protein and compared with birds that were vaccinated with live NDV vaccine. The immune response was evaluated by indirect ELISA, lymphocyte transformation test, virus neutralization test, cytokine analysis, immunophenotyping of peripheral blood mononuclear cells, and protective efficacy study against virulent NDV challenge virus infection. Chickens in prime-boost group developed a higher level of humoral and cellular immune responses as compared with those immunized with plasmid or protein alone. The DNA prime-protein boost using F protein of NDV yielded 91.6% protection against virulent NDV challenge infection better than immunization with DNA vaccine (66.6%) or rF protein (83.3%) alone. These findings suggest that the “DNA prime-protein boost” approach using full-length F gene could enhance the immune response against NDV in the chickens.

Keywords

Newcastle disease virus Fusion protein DNA prime-protein boost Protective immunity 

Notes

Acknowledgements

The authors are thankful to the Director of the Indian Veterinary Research Institute, Izatnagar, for providing necessary facilities and infrastructure.

Funding information

This work was supported by grants from the National Fund for Basic and Strategic Research in Agriculture of ICAR (NFBSFARA/BS-3010) awarded to SD and the Department of Biotechnology, Government of India BT/PR15373/AAQ/57/116/2011 and DBT-JRF/2012-13/107 awarded to MMC and SAK respectively.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Statement of animal rights

Animal experiments were approved by the Institute Animal Ethics Committee of ICAR-Indian Veterinary Research Institute, Izatnagar, and performed in accordance with animal ethics guidelines and approved protocols of the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Ministry of Environment, Forest, Climate change, Government of India.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Sagar A. Khulape
    • 1
  • Hemanta Kumar Maity
    • 2
  • Dinesh Chandra Pathak
    • 2
  • Narayan Ramamurthy
    • 2
  • Saravanan Ramakrishnan
    • 3
  • Madhan Mohan Chellappa
    • 2
    Email author
  • Sohini Dey
    • 2
    Email author
  1. 1.Directorate on Foot and Mouth Disease VirusNainitalIndia
  2. 2.Recombinant DNA Laboratory, Division of Veterinary BiotechnologyIndian Veterinary Research InstituteBareillyIndia
  3. 3.Immunology SectionIndian Veterinary Research InstituteBareillyIndia

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