Evaluation of a fusion gene-based DNA prime-protein boost vaccination strategy against Newcastle disease virus
- 61 Downloads
The low potency of genetic immunization has to date impeded development of commercial vaccines against major infectious diseases. The aim of this study was to develop and evaluate a fusion gene-based DNA prime-protein boost vaccination strategy to improve the efficacy of both DNA and subunit vaccines against Newcastle disease virus (NDV). The fusion (F) protein, a viral surface glycoprotein, is responsible for the cell membrane fusion and spread, also is one of the major targets for immune response. In this study, groups of chickens were vaccinated twice intramuscularly at 14-day interval either with plasmid DNA encoding F protein gene of NDV or with recombinant F protein alone or with plasmid DNA and boosted with the recombinant F protein and compared with birds that were vaccinated with live NDV vaccine. The immune response was evaluated by indirect ELISA, lymphocyte transformation test, virus neutralization test, cytokine analysis, immunophenotyping of peripheral blood mononuclear cells, and protective efficacy study against virulent NDV challenge virus infection. Chickens in prime-boost group developed a higher level of humoral and cellular immune responses as compared with those immunized with plasmid or protein alone. The DNA prime-protein boost using F protein of NDV yielded 91.6% protection against virulent NDV challenge infection better than immunization with DNA vaccine (66.6%) or rF protein (83.3%) alone. These findings suggest that the “DNA prime-protein boost” approach using full-length F gene could enhance the immune response against NDV in the chickens.
KeywordsNewcastle disease virus Fusion protein DNA prime-protein boost Protective immunity
The authors are thankful to the Director of the Indian Veterinary Research Institute, Izatnagar, for providing necessary facilities and infrastructure.
This work was supported by grants from the National Fund for Basic and Strategic Research in Agriculture of ICAR (NFBSFARA/BS-3010) awarded to SD and the Department of Biotechnology, Government of India BT/PR15373/AAQ/57/116/2011 and DBT-JRF/2012-13/107 awarded to MMC and SAK respectively.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
Statement of animal rights
Animal experiments were approved by the Institute Animal Ethics Committee of ICAR-Indian Veterinary Research Institute, Izatnagar, and performed in accordance with animal ethics guidelines and approved protocols of the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Ministry of Environment, Forest, Climate change, Government of India.
- Cattoli, G., Fusaro, A., Monne, I., Molia, S., Le Menach, A., Maregeya, B., Maina, A.G., Koffi, J.N., Thiam, H., Bezeid, O.E., Salviato, A., Nisi, R., Terregino, C. and Capua, I., 2010. Emergence of a new genetic lineage of Newcastle disease virus in West and Central Africa-implications for diagnosis and control, Veterinary Microbiology, 142, 168-176CrossRefGoogle Scholar
- Kamiya, N., Niikura, M., Ono, M., Kai, Ch., Matsuura, Y., and Mikami, T., 1994. Protective effect of individual glycoproteins of Newcastle disease virus expressed in insect cells: the fusion protein derived from avirulent strain had lower protective efficacy, Virus Research, 32, 373-379CrossRefGoogle Scholar
- Lamb, R. and Parks, G., 2007. Paramyxoviridae: the viruses and their replication, In: D.M. Knipe, P.M. Howley, D.E. Griffin, R.A. Lamb, M.A. Martin, Philadelphia: Lippincott Williams & Wilkins, pp 1449–1496Google Scholar
- Lee, Y.J., Sung, H.W., Cho, J.G., Lee, E.K., Yoon, H., Kim, J.H. and Song, C.S., 2008. Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin-neuraminidase proteins, Journal of Veterinary Science, 9, 301-308CrossRefGoogle Scholar
- Liang, R., van den Hurk, J.V., Zheng, C., Yu, H., Pontarollo, R.A., Babiuk, L.A. and van Drunen Littel-van den Hurk, S., 2005. Immunization with plasmid DNA encoding a truncated, secreted form of the bovine viral diarrhea virus E2 protein elicits strong humoral and cellular immune responses, Vaccine, 23, 5252-5262CrossRefGoogle Scholar
- Mattanovich, D., Branduardi, P., Dato, L., Gasser, B., Sauer, M. and Porro, D., 2012. Recombinant Protein Production in Yeasts, In: Lorence A. (eds) Recombinant Gene Expression, Methods in Molecular Biology (Methods and Protocols), 824, Humana Press, Totowa, NJGoogle Scholar
- Sawant, P.M., Verma, P.C., Subudhi, P.K., Chaturvedi, U., Singh, M., Kumar, R. and Tiwari, A.K., 2011. Immunomodulation of bivalent Newcastle disease DNA vaccine induced immune response by co-delivery of chicken IFN-gamma and IL-4 gene, Veterinary Immunology and Immunopathology, 144, 36-44CrossRefGoogle Scholar
- Shedlock, D.J. and Weiner, D.B., 2000. DNA vaccination: antigen presentation and the induction of immunity, Journal of Leukocyte Biology, 68, 793-806Google Scholar