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Tropical Animal Health and Production

, Volume 51, Issue 8, pp 2493–2503 | Cite as

Targeted transcript analysis revealed association of suboptimal expression of certain endometrial immunity-related genes with disparate uterine diseases in zebu cows

  • R. K. BaithaluEmail author
  • S. K. SinghEmail author
  • A. Kumaresan
  • S. Kumar
  • B. R. Maharana
  • S. Mallick
  • T. K. Mohanty
  • A. K. Mohanty
Regular Articles
  • 97 Downloads

Abstract

The onset of uterine infection during postpartum period compromises uterine health, fertility, and productivity of dairy cattle. Endometrial innate immunity plays a key role in eliminating uterine infection and keeping the uterus healthy. Hence, the present study has been designed with the hypothesis that altered endometrial immune response around calving may compromise uterine health during postpartum period. Expression of interleukins (IL-1β, IL-6, IL-8, IL-10, and TNF-α), prostaglandin synthase (PGFS, PGES), and antimicrobial peptides (beta-defensins (BDEF-4, BDEF-5), lingual antimicrobial peptide (LAP), and calcium-binding proteins (S100A8, S100A9, and S100A12) in endometrial tissues on the day of calving was studied using qRT-PCR, and circulating concentrations of prostaglandin E and F metabolites (PGEM and PGFM) during peripartum period (on days − 7, − 4, − 1 (before calving), 0 (on the day of calving), + 1, + 4, and + 7 (post calving)) of normal (healthy) cows (n = 11) that did not develop postpartum uterine infection and cows that developed puerperal metritis (n = 7) and clinical endometritis (n = 6) were studied. Endometrial expression of IL-1β, TNF-α, BDEF-4, BDEF-5, S100A8, S100A12, and PGFS was higher (P < 0.05), and expression of IL-6, IL-8, IL-10, and PGES was lower (P < 0.05) in normal (healthy) cows than puerperal metritic and clinical endometritic cows. The PGFM concentration in serum was high (P < 0.05) on days 0, + 1, and + 4 of calving in puerperal metritic cows followed by normal and clinical endometritic cows. However, PGEM concentration in serum was high (P < 0.05) during peripartum period in uterine-infected (puerperal metritic and clinical endometritic) cows compared with normal cows. From the above findings, it is concluded that higher constitutive expression of IL-1β, TNF-α, PGFS, BDEF-4, BDEF-5, S100A8, and S100A12 genes in the endometrium and lower concentration of PGEM during the period immediate to calving might be beneficial for uterine health of cows.

Keywords

Antimicrobial peptides Interleukins Postpartum uterine health Prostaglandins 

Notes

Acknowledgments

The authors thank the Indian Council of Agricultural Research, Government of India, the Director, ICAR-National Dairy Research Institute, Karnal, Haryana, and the Director, ICAR-Indian Veterinary Research Institute, Bareilly, U.P., India, for providing necessary facilities and logistics support to conduct this study.

Funding information

This study was funded by the National Dairy Research Institute, India.

Compliance with ethical standards

The procedures applied have been approved by the Institute Animal Ethics Committee (1705/Go/ac/13/CPCSEA).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

11250_2019_1958_MOESM1_ESM.docx (11 kb)
ESM 1 (DOCX 10 kb).

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • R. K. Baithalu
    • 1
    Email author
  • S. K. Singh
    • 2
    Email author
  • A. Kumaresan
    • 1
  • S. Kumar
    • 3
  • B. R. Maharana
    • 4
  • S. Mallick
    • 5
  • T. K. Mohanty
    • 1
  • A. K. Mohanty
    • 3
  1. 1.Animal Reproduction, Gynaecology and ObstetricsICAR-National Dairy Research InstituteKarnalIndia
  2. 2.Division of Animal ReproductionICAR-Indian Veterinary Research InstituteIzatnagarIndia
  3. 3.Animal Biotechnology CenterICAR-National Dairy Research InstituteKarnalIndia
  4. 4.Regional Research CenterReferral Veterinary Diagnostic & Extension Centre, LUVASKarnalIndia
  5. 5.Division of Animal PhysiologyICAR-National Dairy Research InstituteKarnalIndia

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