Tropical Animal Health and Production

, Volume 51, Issue 8, pp 2297–2304 | Cite as

Effect of rumen-protected nutrients on feed intake, body weights, milk yield, and composition in Murrah buffaloes during early lactation

  • Gyanendra Singh Katiyar
  • Vishal MudgalEmail author
  • Rakesh Kumar Sharma
  • Anurag Bharadwaj
  • Sushil Kumar Phulia
  • Andonissamy Jerome
  • Inderjeet Singh
Regular Articles


The effect of rumen-protected nutrients (bypass fat, BPF; bypass protein, BPP; or their combination, BPPF) was investigated in Murrah buffaloes during the early stage of lactation. Forty Murrah buffaloes (BW 531.92 ± 10.85 kg) just after parturition were randomly distributed into four groups according to parity and milk production. Buffaloes individually fed ration from day 0 to 90 postpartum according to feeding group and nutrient requirement. Control and BPF fed groups received a concentrate mixture, CM1 with 25% rumen-protected protein (using barley, wheat bran, and mustard oil cake), BPP and BPPF groups received a second concentrate mixture, CM2 with 40% rumen-protected protein (using barley, de-oiled rice bran, and cottonseed cake). Bypass fat fed groups (BPF and BPPF) additionally were supplemented with 15 g BPF (Ca salt of long-chain fatty acids) per kg milk yield in their respective concentrate mixtures. Dry matter intake, body weights, body condition score, and total milk yield were similar between groups (P > 0.05). Fat-corrected milk (FCM) production was improved (14.5%, P > 0.05) in groups fed BPP and BPPF, while significant (19.45%, P < 0.05) improvement was observed in BPF-fed group. Overall mean values of milk fat, solid not fat, protein, lactose, and total solids were found to be high (P < 0.05) in treatment groups as compared with control values. It may be concluded that supplementation with BPP or BPF either alone or in combination positively influences the quality of milk produced in Murrah buffaloes during early lactation and BPF additionally had improvement on the quantitative trait of milk as well.


Buffaloes Bypass fat Bypass protein Intake Milk production Milk quality 



The first author acknowledges the Junior Research Fellowship received by ICAR during the study period.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable institutional guidelines for the care and use of animals were followed.


  1. Association of Official Analytical Chemists, 2000. Official methods of analysis of AOAC International, 17th edition. AOAC International: Gaithersburg, MD, USA.Google Scholar
  2. Beam, S.W. and Butler, W.R., 1997. Energy balance and ovarian follicle development prior to the first ovulation postpartum in dairy cows receiving three levels of dietary fat, Biology of Reproduction, 56, 133–142 ( Scholar
  3. Ben-Salem, M. and Bouraoui, R., 2008. Effects of calcium salts of palm fatty acids and protected methionine supplementation on milk production and composition and reproductive performances of early lactation dairy cows, International Journal of Dairy Sciences, 3, 187–193 ( Scholar
  4. Bhatt, R. S. and Sahoo, A., 2017. Effect of feeding complete feed block containing rumen protected protein, non-protein nitrogen and rumen-protected fat on improving body condition and carcass traits of cull ewes, Journal of Animal Physiology and Animal Nutrition ( Scholar
  5. Chandrasekharaiah, M., Sampath, K.T. and Praveen, U.S., 2008. Effect of feeding bypass protein on milk production performance in crossbred cows, Indian Journal of Animal Sciences, 78, 527–530Google Scholar
  6. Chatterjee, A. and Walli, T.K., 2003. Effect of formaldehyde treatment on effective protein degradability and in vitro post-ruminal digestibility of mustard cake, Indian Journal of Animal Nutrition, 20, 143–148.Google Scholar
  7. Chaturvedi, O.H. and Walli, T.K., 2000. Effect of feeding graded levels of bypass protein on nutrient utilization, nutrient partitioning and on performance of lactating crossbred cows, Indian Journal of Dairy Sciences, 53, 1–5Google Scholar
  8. Chen, K.J., Jan, D.F., Chiou, P.W.S. and Yang, D.W., 2002. Effects of dietary heat extruded soybean meal and protected fat supplement on the production, blood and ruminal characteristics of Holstein cows, Asian Australasian Journal of Animal Science, 15, 821–827CrossRefGoogle Scholar
  9. Chithrima, S.C.R., Jasmine, R.K. and Banakar, P.S., 2016. Effect of bypass fat supplementation during early lactation on milk composition of crossbred dairy cows of Kerala, International Journal of Applied and Pure Science and Agriculture, 2, 158–160Google Scholar
  10. Chouinard, P.Y., Girard, V. and Brisson, G.J., 1997. Lactational response of cows to different concentrations of calcium salts of canola oil fatty acids with or without bicarbonates, Journal of Dairy Science, 80, 1185–1193 (10.3168/jdsS0022-0302(97)76046-6)CrossRefGoogle Scholar
  11. Clark, J.H., 1975. Lactational responses to post-ruminal administration of protein and amino acids. Journal of Dairy Science, 58, 1178–1197 ( Scholar
  12. Desai, V.R., Padheriya, Y.D., Rabari, P.D., Waghela, C.R. and Parnerkar, S., 2017. Effect of feeding bypass fat on milk composition of buffaloes from tribal areas in Panchmahal and Vadodara. Indian Veterinary Journal, 94, 41–43Google Scholar
  13. Garg, M.R., Kumar, S., Sherasia, P.L. and Bhanderi, B.M., 2007. Effect of bypass protein supplementation on production and reproduction of lactating cows under field condition in Kerala, Proceedings of the International Tropical Animal Nutrition Conference, 2007, NDRI, Karnal, India, 276Google Scholar
  14. Goering, H.K. and Van Soest, P.J., 1970. Forage fiber analysis (apparatus, reagents, procedures and some application). ARS U.S. Department of Agriculture. Handbook No. 379. Supdt. of Documents. (U.S Govt. Printing Office, Washington, DC)Google Scholar
  15. Gowda, N.K.S., Manegar, A., Raghavendra, A., Verma, S., Maya, G., Pal, D.T., Suresh, K.P. and Sampath, K.T., 2013. Effect of protected fat supplementation to high yielding dairy cows in field condition, Animal Nutrition and Feed Technology, 13, 125–130Google Scholar
  16. Hess, B.W., Moss, G.E. and Rule, D.C., 2008. A decade of developments in the area of fat supplementation research with beef cattle and sheep, Journal of Animal Science, 86, E188–E204 ( Scholar
  17. ICAR, 2013. nutrient requirements of animals – cattle and buffalo (ICAR- NIANP, India)Google Scholar
  18. Jenkins, T.C. and Palmquist, D.L., 1984. Effect of fatty acids of calcium soaps on rumen and total nutrient digestibility of dairy rations, Journal of Dairy Science, 67, 978–986 ( Scholar
  19. Lohrenz, A.K., Duske, K., Schneider, F., Nürnberg, K., Losand, B., Seyfert, H.M., Metges, C.C. and Hammon, H.M., 2010. Milk performance and glucose metabolism in dairy cows fed rumen-protected fat during mid-lactation, Journal of Dairy Science, 93, 5867–5876CrossRefGoogle Scholar
  20. Lopes, C.N., Scarpa, A.B., Cappellozza, B.I., Cooke R.F. and Vasconcelos, J.L.M., 2009. Effects of rumen-protected polyunsaturated fatty acid supplementation on reproductive performance of Boss indicus beef cows, Journal of Animal Science, 87, 3935–3943 ( Scholar
  21. Lowman, B.G., Scott, N. and Somerville, S., 1976. Condition scoring of cattle. Rev. 6th edition. (The Edinburgh School of Agriculture, Edinburgh, UK)Google Scholar
  22. Mishra, B.B., Swain, R.K., Sahu, B. K. and Sawantaray, D.P., 2006. Effect of bypass protein supplementation on nutrient utilization, milk production and its composition in crossbred cows on paddy straw based ration, Animal Nutrition and Feed Technology, 6, 123–133Google Scholar
  23. Mudgal, V., Baghel, R.P.S., Ganie, A. and Srivastava, S., 2012. Effect of feeding bypass fat on intake and production performance of lactating crossbred cows, Indian Journal of Animal Research, 46, 103–104Google Scholar
  24. Naik, P.K., Saijpaul, S., Sirohi, A.S. and Raquib, M., 2009. Lactation response of crossbred dairy cows fed indigenously prepared rumen protected fat - a field trial, Indian Journal of Animal Sciences, 79, 1045–1049Google Scholar
  25. Parnerkar, S., Kumar, D., Shankhpal, S.S. and Thube, H., 2011. On-farm trial on feeding of bypass protein compound concentrate mixture to buffaloes during early lactation, Proceedings of XIV Biennial Conference of Animal Nutrition Society of India, 2011, GBPUA & T, Pantnagar, India, 60Google Scholar
  26. Rice, V.A., 1970. Breeding and improvement of farm animals (McGraw Hill Bombay, India)Google Scholar
  27. Sartin, J.L., Cummins, K.A., Krernpainen, R.J., Marple, D.N., Rahe, C.H. and William, J.C., 1985. Glucagon, insulin and GH responses to glucose infusion in lactating cows. Nutrition Abstracts and Review (Series B) Abstract No. 4371Google Scholar
  28. Schroeder, G.F., Gagliostro, G.A., Becu Villalobos, Lacau, D. and Mengido, I., 2002. Supplementation with partially hydrogenated oil in grazing dairy cows in early lactation, Journal of Dairy Sciences, 85, 580–594 ( Scholar
  29. Sharma, S., Singh, M., Roy, A.K., Thakur, S., 2016. Effect of prepartum prilled fat supplementation on feed intake, energy balance and milk production in Murrah buffaloes, Veterinary World 9, 256–259 ( Scholar
  30. Shelke, S.K., Thakur, S.S. and Amrutkar, S.A., 2012a. Effect of feeding protected fat and proteins on milk production, composition and nutrient utilization in Murrah buffaloes, Animal Feed Science Technology, 171, 98–107CrossRefGoogle Scholar
  31. Shelke, S.K., Thakur, S.S. and Shete, S.M., 2012b. Productive and reproductive performance of Murrah buffaloes (Bubalus bubalis) supplemented with rumen protected fat and protein, Indian Journal of Animal Nutrition, 29, 317–323Google Scholar
  32. Silvestre, F.T., Carvalh, T.S., Francisco, N., Santos, J.E., Staples, C.R., Jenkins, T.C. and Thatcher, W.W., 2011. Effects of differential supplementation of fatty acids during the peripartum and breeding periods of Holstein cows: I. Uterine and metabolic responses, reproduction, and lactation. Journal of Dairy Sciences, 94, 189–204 ( Scholar
  33. Sirohi, S.K., Walli, T.K. and Mohanta, R.K., 2010. Supplementation effect of bypass fat on production performance of lactating crossbred cows, Indian Journal of Animal Sciences, 80,733–736Google Scholar
  34. Spicer, L.J., Vernon, R.K., Tucker, W.E., Waterman, R.P., Hogue, J.F. and Adams, G.D., 1993: Effects of inert fat on energy balance plasma concentrations of hormones, and reproduction in dairy cows, Journal of Dairy Sciences, 76, 2664–2673 ( Scholar
  35. Sultan, J.I., Javaid, A., Nadeem, M., Akhtar, M.Z. and Mustafa, M.I., 2009. Effect of varying ruminally degradable to ruminally undegradable protein ratio on nutrient intake, digestibility and N metabolism in Nili-Ravi buffalo calves (Bubalus bubalis), Livestock Science, 122, 130–133 ( Scholar
  36. Thakur, S.S. and Shelke, S.K., 2010. Effect of supplementing bypass fat prepared from soybean acid oil on milk yield and nutrient utilization in Murrah buffaloes, Indian Journal of Animal Sciences, 80, 354–357Google Scholar
  37. Thanh, L.P. and Suksombat, W., 2015. Milk production and income over feed costs in dairy cows fed medium-roasted soybean meal and corn dried distiller’s grains with solubles, Asian Australasian Journal of Animal Science, 28, 519–529CrossRefGoogle Scholar
  38. Tyagi, N., Thakur, S.S. and Shelke, S.K., 2009. Effect of feeding bypass fat supplement on milk yield, its composition and nutrient utilization in crossbred cows, Indian Journal of Animal Nutrition, 26, 1–8Google Scholar
  39. Wadhwa, M., Grewal, R.S., Bakshi, M.P.S.and Brar, P.S., 2012. Effect of supplementing bypass fat on the performance of high yielding crossbred cows, Indian Journal of Animal Sciences, 82, 200–203Google Scholar
  40. Waghela, C. R., Parnerkar, S. and Barbadikar, M., 2016. Effect of Feeding Bypass Protein Feed on Milk Production, Composition and Return over Feed Cost from Buffaloes of Tribal Areas in Panchmahal and Vadodara Districts, International Journal of Livestock Research, 6, 63–70CrossRefGoogle Scholar
  41. Walli, T.K., 2008. Bypass protein technology: A success story in feeding of dairy animals for increase milk production as a cheaper cost, Indian Dairyman, 60, 53–60Google Scholar
  42. Wanapat, M., Ngarmsang, A., Korkhuntot, S., Nontaso, N., Wachirapakorn, C., Beakes, G. and Rowlinson, P. 2000. A comparative study on the rumen microbial population of cattle and swamp buffaloes raised under traditional village conditions in the northeast of Thailand, Asian-Australasian Journal of Animal Sciences, 13, 918–921CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Division of Animal Physiology and ReproductionICAR-Central Institute for Research on BuffaloesHisarIndia
  2. 2.Division of Animal Nutrition and Feed TechnologyICAR-Central Institute for Research on BuffaloesHisarIndia
  3. 3.Division of Animal Genetics and BreedingICAR-Central Institute for Research on BuffaloesHisarIndia

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