Molecular identification and detection of Wohlfahrtia magnifica in ovine vulvar myiasis in Gansu, China
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Traumatic myiasis causes substantial economic losses to farmers worldwide. In the present study, six flocks of sheep (2261 sheep) were investigated in Gansu, China, and 207 of 552 larvae were genetically characterized based on three genes, including cyt b, EF-1α, and white gene, by polymerase chain reaction and sequence analysis. A survey of sheep in China revealed that the prevalence of vulvar myiasis of six sheep flocks was 5.00% (11/220, Flock1), 4.85% (10/206, Flock2), 4.50% (9/200, Flock3), 5.00% (15/300, Flock4), 4.68% (15/320, Flock5), 0% (0/1015, Flock6), respectively. The sequence and phylogenetic analysis showed that only Wohlfahrtia magnifica was detected in the field samples. This is the first report of ovine vulvar myiasis caused by W. magnifica in Gansu, China. Some prophylactic measures are strongly recommended to reduce the risk of sheep acquiring traumatic myiasis in Gansu, China.
KeywordsVulvar myiasis Wohlfahrtia magnifica Identification Sheep PCR Gansu
polymerase chain reaction
- cyt b gene
cytochrome b gene
- EF-1α gene
elongation factor 1-alpha gene
The authors would like to thank the director Jiang Zhongwen of the Bureau of Animal Husbandry in Yongchang district for their cooperation during the field-collected samples.
This study was financially supported by the National Key R&D Program of China (2017YFD0501200, 2018YFD0501804, 2018YFD0502305, 2016YFD0501503); NBCITS (CARS-37); Special Funds for Agro-scientific Research in the Public Research (Y2018YJ08-4), MOA; ASTIP, FRIP (2014ZL010), CAAS; 973 Program (2015CB150300), MOST; and State Key Laboratory of Veterinary Etiological Biology Project.
Compliance with ethical standards
Conflict of interests
The authors declare that they have no competing interests.
All animals were handled in strict accordance with good animal practice according to the Animal Ethics Procedures and Guidelines of the People’s Republic of China, and the study was approved by the Animal Ethics Committee of Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (No. LVRIAEC2012-003).
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