Antimicrobial resistance and presence of virulence factor genes in Trueperella pyogenes isolated from pig lungs with pneumonia
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Trueperella pyogenes (T. pyogenes) is a worldwide known pathogen of domestic ruminants and pigs causing a wide variety of infections. The objective of this study was to report the presence of major virulence genes in T. pyogenes isolated from pigs with respiratory clinical signs and determine their resistance to antibiotics at the same time. A total of 27 T. pyogenes strains were obtained from Jilin Province, and the nanH, nanP, cbpA, fimC, and fimE virulence genes were detected in 7 (25.9%), 14 (51.9%), 18 (66.7%), 8 (29.6%), and 16 (59.3%) isolates, respectively. All isolates were observed to harbor plo and fimA genes. However, 27 T. pyogenes strains tested negative for fimG gene. Antibiotic susceptibility tests revealed that the isolated strains had extensive drug resistance, all isolates were sensitive to fluoroquinolones and penicillins antibiotics, and high levels of resistance were found to gentamicin (77.8%), amikacin (74.1%), erythromycin (85.2%), and azithromycin (85.2%). These results highlights the need for prudent use of specific antimicrobial agents in veterinary clinical treatment.
KeywordsAntimicrobial resistance Trueperella pyogenes virulence factors
We thank Zhi-chao ZHAI and Jie LIU for their laboratory contributions.
This research was funded by National Key Research and Development Plan (Grant number 2016YFD0501301), National Natural Science Foundation of China (Grant number 31702293), the Science and Technology Development Plan of Jilin Province (Grant number 20170520074JH), Jilin Provincial Education Department “the 13th five-year” Science and Technology Project (Grant number JJKH20180696KJ), and Jilin Agricultural University Research Foundation (Grant number 201702).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflicts of interest.
All procedures performed with the animals of the present experiment were in accordance with the ethical standards established by the committee of Jilin Agriculture University.
- Hijazin, M., Ulbegi-Mohyla, H., Alber, J., Lämmler, C., Hassan, A. A., Abdulmawjood, A., Prenger-Berninghoff, E., Weiss, R. and Zschöck, M., 2011. Molecular identification and further characterization of Arcanobacterium pyogenes isolated from bovine mastitis and from various other origins. Journal of Dairy Science, 94: 1813–1819.CrossRefGoogle Scholar
- Moreno, L. Z., Matajira, C. E. C., Da Costa, B. L. P., Ferreira, T. S. P., Silva, G. F. R., Dutra, M. C., Gomes, V. T. M., Silva, A. P. S., Christ, A. P. G., Sato, M. I. Z. and Moreno, A. M., 2017. Characterization of porcine Trueperella pyogenes by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALD-TOF MS), molecular typing and antimicrobial susceptibility profiling in sao paulo state. Comparative Immunology, Microbiology and Infectious Diseases, 51: 49–53.CrossRefGoogle Scholar
- Risseti, R. M., Zastempowska, E., Twarużek, M., Lassa, H. , Pantoja, J. C. F., De Vargas, A. P. C., Guerra, S.T., Bolaños, C. A. D., de Paula, C.L., Alves, A.C., Colhado, B.S., Portilho, F. V. R., Tasca, C., Lara, G. H. B. and Lara, G. H. B., 2017. Virulence markers associated with Trueperella pyogenes infections in livestock and companion animals. Letters in Applied Microbiology, 65: 125–132.CrossRefGoogle Scholar
- Rogovskyy, A. S., Lawhon, S., Kuczmanski, K., Gillis, D. C., Wu, J., Hurley, H., Rogovska, Y. V., Konganti, K., Yang, C. Y. and Duncan, K., 2018. Phenotypic and genotypic characteristics of Trueperella pyogenes isolated from ruminants. Journal of Veterinary Diagnostic Investigation Official Publication of the American Association of Veterinary Laboratory Diagnosticians Inc, 30: 348–353.Google Scholar
- Silva, E., Gaivão, M., Leitão, S., Jost, B. H., Carneiro, C., Vilela, C. L., Lopes da Costa, L. and Mateus, L., 2008. Genomic characterization of Arcanobacterium pyogenes isolates recovered from the uterus of dairy cows with normal puerperium or clinical metritis. Veterinary Microbiology, 132: 111–118.CrossRefGoogle Scholar
- Wareth, G., El-Diasty, M., Melzer, F., Murugaiyan, J., Abdulmawjood, A., Sprague, L. D. and Neubauer, H., 2018. Trueperella pyogenes and Brucella abortus coinfection in a dog and a cat on a dairy farm in Egypt with recurrent cases of mastitis and abortion. Veterinary Medicine International, 2018: 2056436.CrossRefGoogle Scholar
- Wickhorst, J.P., Hassan, A. A., Sheet, O. H., Eisenberg, T., Sammra, O., Alssahen, M., Lämmler, C., Prenger-Berninghoff, E., Zschöck, M., Timke, M. and Abdulmawjood, A., 2018. Trueperella pyogenes isolated from a brain abscess of an adult roebuck (Capreolus capreolus). Folia Microbiologica, 63: 17–22.CrossRefGoogle Scholar
- Zastempowska, E. and Lassa, H., 2012. Genotypic characterization and evaluation of an antibiotic resistance of Trueperella pyogenes (Arcanobacterium pyogenes) isolated from milk of dairy cows with clinical mastitis. Veterinary Microbiology, 161.Google Scholar