Tropical Animal Health and Production

, Volume 51, Issue 7, pp 1997–2001 | Cite as

Protection conferred by a vaccine derived from an inactivated Egyptian variant of infectious bronchitis virus: a challenge experiment

  • Emad Al-Ebshahy
  • Mohammed Abdel-Sabour
  • Osama AbasEmail author
  • Tokuma Yanai
Regular Articles


The current study investigated the protective efficacy of a formalin-inactivated infectious bronchitis virus (IBV) vaccine derived from the field strain KP729422, which exhibits low S1 spike protein sequence homology (77.1–79.8%) with the currently used vaccine strains in Egypt. Two-week-old, specific-pathogen-free chickens were subcutaneously inoculated with a single dose of the vaccine containing 106.7 50% embryo infective dose (EID50) of the inactivated virus. At 6 weeks of age, the chickens were challenged with 104 EID50 of the same virus strain via the oculonasal route. In comparison with the unvaccinated challenged group, the vaccinated chickens had significantly higher IBV-neutralizing antibody titers and exhibited efficient protection against challenge on the basis of tracheal ciliary activity. However, the challenge virus was recovered from the kidneys and tracheas of these chickens at rates of 40% and 60%, respectively. These findings suggest that a single application of the vaccine may provide sufficient clinical and respiratory protection, but may not ensure complete protection against infection by the challenge virus.


Infectious bronchitis virus Variant Inactivated vaccine Protection 


Compliance with ethical standards

Conflict of interest

The author declares that there are no conflicts of interest.

Ethical statement

The experimental procedures were performed in accordance with the ethical standards applied in Veterinary Serum and Vaccine Research Institute, Abbassia, Egypt.


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Emad Al-Ebshahy
    • 1
  • Mohammed Abdel-Sabour
    • 2
  • Osama Abas
    • 3
    Email author
  • Tokuma Yanai
    • 4
  1. 1.Department of Microbiology, Faculty of Veterinary MedicineAlexandria UniversityAlexandriaEgypt
  2. 2.Veterinary Serum and Vaccine Research InstituteCairoEgypt
  3. 3.Department of Animal Medicine, Faculty of Veterinary MedicineAlexandria UniversityAlexandriaEgypt
  4. 4.Laboratory of Veterinary Pathology, Faculty of Applied Biological SciencesGifu UniversityGifuJapan

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