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The effect of haplotypes in the promoter region of SIRT4 gene on the ultrasound traits in Qinchuan cattle

  • Linsheng Gui
  • Hua Wu
  • Sayed Haidar Abbas RazaEmail author
  • Nicola M. Schreurs
  • Mujahid Ali Shah
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Abstract

Sirtuin 4 (SIRT4) belongs to the mitochondrial sirtuin class of NAD+-dependent protein deacylases. This gene plays an important role in the regulation of lipid metabolism, cellular growth, and metabolism in mammals. Here, potential polymorphisms were sought in the bovine SIRT4 gene, and the relationships between the detected polymorphisms and carcass quality in Qinchuan cattle were assessed. Four single nucleotide polymorphisms (SNPs) were identified in the promoter region of the SIRT4 gene from the sequencing results of 452 individual cattle. A total of 8 different haplotypes were identified. Of these, the 3 most frequently observed haplotypes had frequencies of 35.0% (-CTG-), 18.3% (-CTA-), and 12.9% (-CCG-). The frequencies of g.-311C > T, g.-771C > T, and g.-1022G > A conformed to Hardy-Weinberg equilibrium in all the samples (chi-square test, P < 0.05). The association analysis indicated that these 3 polymorphisms were significantly associated with subcutaneous fat depth and intramuscular fat content (at P < 0.01 or P < 0.05). Interestingly, the Hap1/2 (-CAG-CAA-) diplotype was more highly associated with desirable ultrasound than other haplotype combinations.

Keywords

SIRT4 gene Polymorphisms Ultrasound traits Chinese Qinchuan cattle 

Notes

Authors’ contributions

Linsheng Gui and Sayed Haidar Abbas Raza designed the experiment and performed the experiments. Linsheng Gui and Sayed Haidar Abbas Raza wrote the manuscript, assisted in analyzing the data, and provided constructive suggestions for the discussion. Nicola M. Schreurs was involved in interpretation of data and drafting the manuscript .Nicola M. Schreurs, Hua Wu, and Mujahid Ali Shah revised the paper critically for intellectual content and also assisted in the modification. All authors read and approved the final manuscript.

Funding

This work was supported by National Natural Science Foundation of Qinghai Province (2018-ZJ-922Q and 2018-ZJ-759), the Project of Qinghai Science and Technology Department (2016-ZJ-Y01), the Open Project of State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University (2018-ZZ-08), and Weng Hongwu Original Research Fund of Peking University (WHW201509).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.State Key Laboratory of Plateau Ecology and AgricultureQinghai UniversityXiningPeople’s Republic of China
  2. 2.College of Animal Science and TechnologyNorthwest A&F UniversityYanglingPeople’s Republic of China
  3. 3.School of Agriculture and EnvironmentMassey UniversityPalmerston NorthNew Zealand
  4. 4.College of Animal Sciences Zhejiang University ZijingangHangzhouChina

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