Advertisement

Novel report on mutation in exon 3 of myostatin (MSTN) gene in Nilagiri sheep: an endangered breed of South India

  • Amiya Ranjan SahuEmail author
  • Jeichitra V.
  • Rajendran R.
  • Raja A.
Regular Articles

Abstract

The variability in breeding program leads to rapid loss of genetic potential for which National Bureau of Animal Genetic Resources is emphasized to conserve the indigenous breeds. The variation in myostatin (MSTN) gene and its association with growth traits will throw light on its potential use as marker in selection. Hence, the study was conducted to detect polymorphism in exon 3 of MSTN, one of the most important growth regulatory gene and its association with growth in Nilagiri sheep breed. Blood samples were collected from Nilagiri sheep (n = 103) of South India and growth data up to 1 year of age was recorded. Genomic DNA was isolated and amplified for part of MSTN gene; PCR products were genotyped by restriction digestion (MspI) and confirmed by sequencing. Restriction digestion has revealed a single nucleotide polymorphism at locus G5622C in exon 3 which was confirmed by sequencing. The wild-type DNA molecule (MM) cleaved by MspI produced 301-bp and 314-bp fragments and those with mutation (mm) would remain undigested. The genotypic frequencies were MM (0.689) and Mm (0.311) with complete absence of mm genotype; and allelic frequencies were M (0.8445) and m (0.1555). The locus was in Hardy-Weinberg equilibrium. The association analysis revealed that there was no significant difference in mean birth, weaning, 6-, 9-, and 12-month weight between MM and Mm genotypes at g.5622G>C locus of exon 3 of MSTN gene. This is the first report of mutation in exon 3 of MSTN gene. The non-significant effect and absence of mm genotype at this locus needs further studies based on large population size and haplotype analysis.

Keywords

Association analysis Myostatin gene Nilagiri sheep PCR-RFLP SNP 

Notes

Acknowledgments

The authors are thankful to the Sheep Breeding Research Station, Sandynallah, TANUVAS, for the necessary support to carry out the research work.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. 19th Livestock Census, 2012. Department of Animal Husbandry and Dairying, Ministry of Agriculture, Government of India, New Delhi.Google Scholar
  2. Archibald, A.L., Cockett, N.E., Dalrymple, B.P., Faraut, T., Kijas, J.W., Maddox, J.F., McEwan, J.C., Hutton, Oddy V., Raadsma, H.W., Wade, C., Wang, J., Wang, W., Xun, X., 2010. The sheep genome reference sequence: a work in progress. Animal Genetics, 41, 449–453.CrossRefGoogle Scholar
  3. Bellinge, R.H.S., Liberles, D.A., Iaschi, S.P.A., Obrien, A., Tay, G.K., 2005. Myostatin and its implications on animal breeding: a review. Animal Genetics, 36, 1–6.CrossRefGoogle Scholar
  4. Bhatia, S., Arora, R. 2005. Biodiversity and conservation of Indian sheep genetic resources – an overview. Asian-Australasian Journal of Animal Sciences, 18, 1387–1402.CrossRefGoogle Scholar
  5. Broad, T.E., Glass, B.C., Greer, G.J., Robertson, T.M., Bain, W.E., Lord, E.A., McEwan, J.C., 2000. Search for a locus near to myostatin that increases muscling in Texel sheep in New Zealand. Proceedings of New Zealand Society of Animal Production, 60, 110–112.Google Scholar
  6. Dehnavi, E., Azari, M.A., Hasani, S., Nassiry, M.R., Mohajer, M., Ahmadi, A.K., Shahmohamadi, L., Yousefi, S., 2012. Polymorphism of myostatin gene in Intron 1 and 2 and Exon 3, and their associations with yearling weight, using PCR-RFLP and PCR-SSCP techniques in Zel sheep. Biotechnology Research International. Article ID 472307, 5 pages.  https://doi.org/10.1155/2012/472307.
  7. Falconer, D.S., Mackay, T.F.C., 1996. Introduction to Quantitative Genetics (4th Ed.). Longman Group Limited, England.Google Scholar
  8. Gan, S.Q., Du, Z., Liu, S.R., Yang, Y.L., Shen, M., Wang, X.H., Yin, J.L., Hu, X.X., Fei, J., Fan, J.J., Wang, J.H, He, Q.H., Zhang, Y.S., Li, N., 2008. Association of SNP haplotypes at the myostatin gene with muscular hypertrophy in sheep. Asian-Australasian Journal of Animal Sciences, 21, 928–935.CrossRefGoogle Scholar
  9. Ganesakale, D., Rathnasabapathy, V., 1973. Sheep breeds of Tamil Nadu. Cheiron, 2, 146–155.Google Scholar
  10. Girish, H., Sivaselvam, S.N., Karthickeyan, S.M.K. Saravanan, R., 2007. Molecular Characterisation of Nilagiri Sheep (Ovis aries) of South India Based on Microsatellites. Asian-Australasian Journal of Animal Sciences, 20 (5), 633–637.CrossRefGoogle Scholar
  11. Grobet, L., Pncelet, D., Royo, L.J., Brouwers, B., Pirottin, D., Michau, C., Menissier, F., Zanotti, M., Dunner, S., George, M., 1998. Molecular definition of an allelic series of mutations disrupting the myostatin function and causing double-muscling. Mammalian Genome, 9, 210–213.CrossRefGoogle Scholar
  12. Hadjipavlou, G., Matika, O., Clop, A., Bishop, S.C., 2008. Two single nucleotide polymorphisms in the myostatin (GDF8) gene have significant association with muscle depth of commercial Charollais sheep. International Society for Animal Genetics. Animal Genetics, 39, 346–353.CrossRefGoogle Scholar
  13. Harvey, W.R., 1990. Mixed Model Least-squares and Maximum Likelihood Computer Programme.PC-2 version. Ohio State University, Colombus.Google Scholar
  14. Kambadur, R., Sharma, M., Smith, T.P.L., Bass, J.J., 1997. Mutations in myostatin (GDF8) in double-muscled Belgian Blue and Piedmontese cattle. Genome Research, 7, 910–916.CrossRefGoogle Scholar
  15. Li, XL, Wu, ZL, Gong, YF, Liu, YQ, Liu, ZZ, Wang, XJ, Xin, TR, Ji, Q., 2006. Single nucleotide polymorphism identification in the caprine myostatin gene. Journal of Animal Breeding and Genetics, 123, 141–144.CrossRefGoogle Scholar
  16. Littlewood, R.W., 1936. Livestock of Southern India. Government of Madras, Madras, pp. 202–216.Google Scholar
  17. Masoudi, A., Hemrani, H., Abbasi, A., 2005. Using PCR-SSCP techniques to study polymorphism of myostatin gene and its association with production traits in Baluchi sheep. Proceedings of 4th National Biotechnology Congress, Iran, Kerman, Iran.Google Scholar
  18. McPherron, A.C., Lee, S.J., 1997. Double muscling in cattle due to mutations in the myostatin gene. Proceedings of the National Academy of Sciences, USA, 94, 12457–12461.CrossRefGoogle Scholar
  19. Rao, K.M.V., Schinckel, P.G., Clarke, W.H., 1960. The wool follicle population of some Indian breeds of sheep. Australian Journal of Agricultural Research, 11, 99–104.Google Scholar
  20. Report, 2008. Sheep Breeding Research Station, Sandynallah. TANUVAS, Chennai, (available at http://www.tanuvas.ac.in/SBRS.html).
  21. Sahu, A.R., Jeichitra, V., Rajendran, R., Raja, A., 2017. Polymorphism in exon 3 of myostatin (MSTN) gene and its association with growth traits in Indian sheep breeds. Small Ruminant Research, 149, 81–84.CrossRefGoogle Scholar
  22. Sambrook, J.E., Fritsch, F., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual, 2nd Ed. Cold spring Harbor Laboratory Press, New York, USA.Google Scholar
  23. Singh, V.K., Mishra, A.K., Kumar, S., 2005. Genetic improvement of sheep for wool and mutton production in India: A Review. Indian Journal of Animal Sciences, 75 (3), 356–364.Google Scholar
  24. Sudhakar, A. Rajendran, A., Rahumathulla, P.S., 2013. Detection of Booroola (FecB) mutation in Indian sheep – Nilagiri. Small Ruminant Research, 113, 55–57.CrossRefGoogle Scholar
  25. Task Force Report, 1996. Report on the Task Force on sheep, goat and rabbit production, Ministry of Agriculture, Department of Animal Husbandry & Dairying, Govt. of India.Google Scholar
  26. Venkataramanan, R., Subramanian, A., Sivaselvam, S.N., Sivakumar, T., Sreekumar, C., Anilkumar, R., Iyue, M., 2013. Pedigree analysis of the Nilagiri sheep of South India. Animal Genetic Resources, 53, 11–18.CrossRefGoogle Scholar
  27. Zhou, H., Hickford, J.G.H., Fang, Q., 2008. Variation in the coding region of the myostatin (GDF8) gene in sheep. Molecular and Cellular Probes, 22, 67–68.CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Animal Genetics and BreedingICAR-NRC on PigGuwahatiIndia
  2. 2.Animal Genetics and BreedingPost Graduate Research Institute in Animal SciencesKattupakkam, KancheepuramIndia
  3. 3.Animal BiotechnologyMadras Veterinary CollegeChennaiIndia

Personalised recommendations