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Bacterial isolation of milk samples submitted from clinical mastitis buffaloes during 2007 to 2016

  • P. S. Bhutia
  • B. K. Bansal
  • D. K. Gupta
  • Raj Sukhbir SinghEmail author
  • S. K. Uppal
Regular Articles
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Abstract

The present study determined trends in the isolation of bacterial pathogens in milk samples submitted from clinical mastitis buffaloes at the University mastitis laboratory during 2007 to 2016. Culture results (n = 3945) were classified into six categories (Staphylococcus spp., 28.7%; Streptococcus spp., 1%; Corynebacterium spp., 1.1%; gram-negative organisms, 3.2%; others including mixed growths, 3.3%; and no growth, 62.7%). Overall, culture results were significantly associated with year (χ2 = 112.28; df = 10; P < 0.001) and season (χ2 = 136.03; df = 20; P < 0.001). From 2007–2009 to 2013–2016, the isolation of Staphylococcus spp. increased from 28.2 to 48.4%, while isolation of Streptococcus spp. (50 to 20%) and Corynebacterium spp. (65.1 to 16.3%) decreased. The proportion of samples characterized as no growth increased from 26.4% (2007–2009) to 46.3% (2013–2016). Further, the proportion of clinical mastitis cases increased over the study years (goodness-of-fit χ2 = 59; df = 2; P < 0.001). With respect to season, the proportion of Staphylococcus spp. (30.6%; 346/1132), Corynebacterium spp. (48.8%; 21/43), and gram-negative bacteria (55.9%; 71/127) were more associated with the monsoon, and that of Streptococcus spp. (47.5%; 19/40) with the winter. Seasonal effects were also observed on the occurrence of clinical mastitis cases; comparatively, more clinical mastitis cases (35.6%; 523/1473) were observed during the monsoon season (goodness-of-fit χ2 = 276.7; df = 4; P < 0.001) as compared with other seasons. The present study concludes that Staphylococcus spp. are the major cause of intramammary infections in buffaloes with clinical mastitis, demanding more studies regarding its epidemiology and pathogenicity. Also, more efforts are needed in the prevention and control of clinical mastitis in buffaloes, especially, during the monsoon season.

Keywords

Buffalo Clinical mastitis Milk sample Bacteriological analysis Year Season 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • P. S. Bhutia
    • 1
  • B. K. Bansal
    • 1
  • D. K. Gupta
    • 1
  • Raj Sukhbir Singh
    • 2
    Email author
  • S. K. Uppal
    • 1
  1. 1.Department of Veterinary Medicine, College of Veterinary ScienceGuru Angad Dev Veterinary and Animal Sciences UniversityLudhianaIndia
  2. 2.Department of Teaching Veterinary Clinical Complex, College of Veterinary ScienceGuru Angad Dev Veterinary and Animal Sciences UniversityLudhianaIndia

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