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Vertical transmission of Neospora caninum in bovine fetuses from a slaughterhouse in Brazil

  • Lanna Vivien de Aquino Diniz
  • Ana Flávia Minutti
  • Beatriz de Souza Lima Nino
  • Letícia Ramos Costa
  • Maria Rachel Melo Bosculo
  • Breno Fernando Martins de Almeida
  • João Luis Garcia
  • Luiz Daniel de BarrosEmail author
Short Communications
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Abstract

Neospora caninum is considered as one of the main causes of reproductive failure in cattle. Vertical transmission is the main route of infection in the bovine host and plays an important role in maintaining the parasite in the herd. Molecular detection of N. caninum is important to determine the occurrence of the disease and to evaluate the genetic diversity of the parasite. The present study aimed at assessing the vertical transmission of N. caninum using molecular techniques to detect the parasite in tissue samples from bovine fetuses collected in a slaughterhouse in the state of São Paulo, Brazil. Seventy fetuses and 70 blood samples from pregnant cows were collected in a slaughtering line. Fresh samples of heart and brain tissue from fetuses were analyzed using molecular assays. Serum samples from fetuses and cows were subjected to an indirect fluorescent antibody test (IFAT) to detect antibodies against N. caninum. Nested PCR targeting the internal transcriber 1 (ITS1) region of the protozoan organism was used in the molecular testing. From the total of fetuses examined, 71.42% were positive for N. caninum by PCR. A higher number of heart samples (47.1%) were positive for the parasite using this technique. Antibodies against the protozoa were detected in 12.9% of serum samples of cows; 2.8% of fetuses were seropositive for this pathogen. Our results show that vertical transmission of N. caninum occurs in cattle from this region of Brazil, and that the use of different diagnostic techniques contributes to successful diagnosis of congenital transmission of the parasite in cattle.

Keywords

Neosporosis IFAT PCR Congenital transmission 

Notes

Funding information

We would like to thank the Fundação de Amparo à Pesquisa do Estado de São Paulo for financial support (FAPESP-IC no. 2016/10938-4).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Lanna Vivien de Aquino Diniz
    • 1
  • Ana Flávia Minutti
    • 2
  • Beatriz de Souza Lima Nino
    • 2
  • Letícia Ramos Costa
    • 1
  • Maria Rachel Melo Bosculo
    • 1
  • Breno Fernando Martins de Almeida
    • 1
  • João Luis Garcia
    • 2
  • Luiz Daniel de Barros
    • 1
    • 2
    Email author
  1. 1.Department of Veterinary MedicineFaculdades Integradas de OurinhosOurinhosBrazil
  2. 2.Department of Preventive Veterinary Medicine, Laboratory of Animal ProtozoologyUniversidade Estadual de LondrinaLondrinaBrazil

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