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Welfare and pregnancy rate of ewes undergoing transcervical artificial insemination with ketamine subarachnoid anesthesia

  • Renata P. B. Carneiro
  • Gustavo G. Macedo
  • Rafael DeRossiEmail author
  • Eliane V. Costa-e-Silva
  • Maria Inês L. Souza
Regular Articles
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Abstract

This study aimed to evaluate the impact of subarachnoid anesthesia with ketamine during transcervical artificial insemination (TCAI) on the welfare of ewes and on subsequent pregnancy rates. Ninety Suffolk adult ewes were randomized into three treatment groups: control group (CG), which underwent the TCAI procedure as established by cervical traction (CG; n = 30) and two groups that received subarachnoid anesthesia with ketamine at a dose of either 0.75 mg/kg (KE0.75; n = 30) or 1.5 mg/kg (KE1.5; n = 30) 5 min before the cervical traction procedure. Intrauterine insemination was performed using frozen semen from three males previously analyzed and approved for fertility. The use of subarachnoid anesthesia decreased ewes’ vocalizations (P = 0.0001) and abdominal contraction (P = 0.0150) during cervical manipulations. The CG had more groans and vocalizations at the moment of cervix clamping and applicator passage through the cervix (P = 0.001). The cervix traction was facilitated by anesthesia. For the control group, most of the cervical traction was done just up to the middle of the vagina (P = 0.0021). Pregnancy rates increased significantly with anesthesia (P = 0.04) as shown by the rates of 40.0%, 56.7%, and 66.7% for CG, KE1.5, and KE0.75, respectively. The CG showed behaviors associated with absolute immobility, which is suggestive of distress. In brief, the use of ketamine in subarachnoid anesthesia for transcervical artificial insemination in ewes facilitated cervical traction, increased the pregnancy rate, and improved animal welfare.

Keywords

Fertility Pain Reproduction technologies Stress Welfare 

Notes

Acknowledgements

The authors thank Mr. Celso Cortada Cordenonssi, owner of the Angélica Ranch, for the ewes used for this study.

Funding information

The authors thank Foundation of Teaching, Science and Technology Development, Mato Grosso do Sul State (FUNDECT) for the financial support and scholarship program (R.P.B.C. is FUNDECT fellow) on context of studies in the reproduction area of small ruminants. This study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil (CAPES) - Finance Code 001.

Compliance with ethical standards

All animal procedures described were approved by The Institutional Animal Care and Use Committee at Federal University of Mato Grosso do Sul (CEUA, prot. 292/2011).

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Álvarez, M., Chamorro, C.A., Kaabi, M., Anel-López, L., Boixo, J.C., Anel, E., Anel, L., and de Paz, P., 2012. Design and “in vivo” evaluation of two adapted catheters for intrauterine transcervical insemination in sheep. Animal Reproduction Science, 131, 153–159.CrossRefGoogle Scholar
  2. Anel, L., Alvarez, M., Martinez-Pastor, F., Garcia-Macias, V., Anel, G., and de Paz, P., 2006. Improvement strategies in ovine artificial insemination. Reproduction in Domestic Animals, 41, 30–42.CrossRefGoogle Scholar
  3. Anel, L., Kaabi, M., Abroug, B., Alvarez, M., Anel, E., Boixo, J.C., de la Fuente, L.F., and de Paz, P., 2005. Factors influencing the success of vaginal and laparoscopic artificial insemination in churra ewes: a field assay. Theriogenology, 63, 1235–1247.CrossRefGoogle Scholar
  4. Barbas, J.P., Gonçalves, S.C., Baptista, M.C., Marques, C.C., Vasques, M.I., and Horta, A.E.M., 2003. Effect of vaginal application of cervix dilating agents (misoprostol and terbutalin) on AI results in ovine Portuguese local breeds. Revista Portuguesa de Ciências Veterinárias, 98, 185–188.Google Scholar
  5. Bartlewski, P.M., and Candappa, I.B.R., 2015. Assessing the usefulness of prostaglandin E2 (Cervidil) for transcervical artificial insemination in ewes. Theriogenology, 84, 1594–1602.CrossRefGoogle Scholar
  6. Battaglia, D.F., Bowen, J.M., Krasa, H.B., Thrun, L.A., Viguie, C., and Karsch, F.J., 1997. Endotoxin inhibits the reproductive neuroendocrine axis while stimulating adrenal steroids: a simultaneous view from hypophyseal portal and peripheral blood. Endocrinology, 138, 4273–4281.CrossRefGoogle Scholar
  7. Breen, K.M., Billings, H.J., Wagenmaker, E.R., Wessinger, E.W., and Karsch, F.J., 2005. Endocrine basis for disruptive effect of cortisol on preovulatory events. Endocrinology, 146, 2107–2115.CrossRefGoogle Scholar
  8. Bristow, D.J., and Holmes, D.S., 2007. Cortisol levels and anxiety-related behaviors in cattle. Physiology & Behavior, 90, 626–628.CrossRefGoogle Scholar
  9. Campbell, J.A., Harvey, T.G., McDonald, M.F., and Sparksman, R.I., 1996. Transcervical insemination in sheep: an anatomical and histological evaluation. Theriogenology, 45, 1535–1544.CrossRefGoogle Scholar
  10. Candappa, I., Bainbridge, H., Price, N., Hourigan, P., and Bartlewski, P., 2009. A preliminary study on the suitability of Cervidil to induce cervical dilation for artificial insemination in ewes. Research in Veterinary Science, 87, 204–220.CrossRefGoogle Scholar
  11. Carlson, N.R., 2002. Os transtornos de Ansiedade, o Autismo e o Estresse. In: Fisiologia do Comportamento (7 eds., Editora Manole, São Paulo), 557–581.Google Scholar
  12. Casali, R., Pinczak, A., Cuadro, F., Guillen-Muñoz, J.M., Mezzalira, A., and Menchaca, A., 2017. Semen deposition by cervical, transcervical and intrauterine route for fixed-time artificial insemination (FTAI) in the ewe. Theriogenology, 103, 30–35.CrossRefGoogle Scholar
  13. CBRA - Colégio Brasileiro de Reprodução Animal, 2013. Manual para Exame Andrológico e Avaliação de Sêmen Animal. third ed., Belo Horizonte, 104 p.Google Scholar
  14. DeRossi, R., Bertoni, R.A., Ruzzon, R.H.S., Verde-Selva, A.B., and Frazilio, F.O., 2010. Segmental dorsolumbar epidural analgesia via the caudal approach using multiple port catheters with ketamine or lidocaine or in combination in cattle. Veterinary Anaesthesia and Analgesia, 37, 451–459.CrossRefGoogle Scholar
  15. DeRossi, R., Carneiro, R.P.B., Ossuna, M.R., Zanenga, N.F., Alves, O.D., Jorge, T.P., Costa e Silva, E.V., and Vasconcelos, J., 2009. Subarachnoid ketamine administration combined with or without misoprostol/oxytocin to facilitate cervical dilation in ewes: a case study. Small Ruminant Research, 83, 74–78.CrossRefGoogle Scholar
  16. DeRossi, R., Junqueira, A.L., and Beretta, M.P., 2005. Use of ketamine or lidocaine or in combination for subarachnoid analgesia in goats. Small Ruminant Research, 59, 95–101.CrossRefGoogle Scholar
  17. DeRossi, R., Pompermeyer, C.T.D., Silva-Neto, A.B., Barros, A.L.C., Jardim, P.H.A., and Frazílio, F.O., 2012. Lumbosacral epidural magnesium prolongs ketamine analgesia in conscious sheep. Acta Cirúrgica Brasileira, 27, 137–143.CrossRefGoogle Scholar
  18. Grant, C., 2004. Behavioural responses of lambs to common painful husbandry procedures. Applied Animal Behaviour Science, 87, 255–273.CrossRefGoogle Scholar
  19. Gündüz, M.C., Turna, O., Cirit, Ü., Uçmak, M., Tek, Ç., Sabuncu, A., and Bacinoḡlu, S., 2010. Lambing rates and litter size following carazolol administration prior to insemination in Kivircik ewes. Animal Reproduction Science, 118, 32–36.CrossRefGoogle Scholar
  20. Habibian, S., Bigham, A.S., and Aali, E., 2011. Comparison of lidocaine, tramadol, and lidocaine-tramadol for epidural analgesia in lambs. Research in Veterinary Science, 91, 434–438.CrossRefGoogle Scholar
  21. Hargreaves, A.L., and Hutson, G.D., 1990. The stress response in sheep during routine handling procedures. Applied Animal Behavior, 26, 83–90.CrossRefGoogle Scholar
  22. Hill, J.R., Thompson, J.A., and Perkins, N.R., 1998. Factors affecting pregnancy rates following laparoscopic insemination of 28,447 merino ewes under commercial conditions: a survey. Theriogenology, 49, 697–709.CrossRefGoogle Scholar
  23. Kershaw, C.M., Khalid, M., McGowan, M.R., Ingram, K., Leethongdee, S., Wax, G., and Scaramuzzi, R.J., 2005. The anatomy of the sheep cervix and its influence on the transcervical passage of an inseminating pipette into the uterine lumen. Theriogenology, 64, 1225–1235.CrossRefGoogle Scholar
  24. Macfarlane, M.S., Breen, K.M., Sakurai, H., Adams, B.M., and Adams, T.E., 2000. Effect of duration of infusion of stress-like concentrations of cortisol on follicular development and the preovulatory surge of LH in sheep. Animal Reproduction Science, 63, 167–175.CrossRefGoogle Scholar
  25. Minton, J.E., Apple, J.K., Parsons, K.M., and Blecha, F., 1995. Stress associated concentrations of plasma cortisol cannot account for reduced lymphocyte function and changes in serum enzymes in lambs exposed to restraint and isolation stress. Journal of Animal Science, 73, 812–817.CrossRefGoogle Scholar
  26. Mohr, E., and Krzywanek, H., 1995. Endogenous oscillator and regulatory mechanisms of body temperature in sheep. Physiology & Behavior, 57, 339–347.CrossRefGoogle Scholar
  27. Muir III, W.W., and Gaynor, J.S., 2009. Comportamento da dor. In: Muir III, W.W and , Gaynor, J.S., Manual de controle da dor em medicina veterinária (ed., MedVet, São Paulo), 62–77.Google Scholar
  28. Murray, R.D., and Ward, W.R., 1993. Welfare implications of modern artificial breeding techniques for dairy cattle and sheep. Veterinary Record, 133, 283–286.CrossRefGoogle Scholar
  29. Nunez, C.M.,(2009) Disposição introduzida em mini expansor aplicador cervical de sêmen para cabras, ovelhas e similares. Available at <http://www.patentesonline.com.br/disposicao-introduzida-em-mini-expansor-e-aplicador-cervical-de-sêmen-para-cabras-13709.html> (accessed 03.11. 09).
  30. Piccione, G., and Caola, G., 2003. Influence of shearing on the circadian rhythm of body temperature in the sheep. Journal of Veterinary Medicine A, 50, 235–240.CrossRefGoogle Scholar
  31. Rabassa, V.S., Tabeleão, V.C., Pfeifer, L.F.M., Schneider, A., Ziguer, E.A., Schossler, E., Severo, N.C., del Pino, A.B., and Corrêa, M.N., 2007. Efeito das técnicas transcervical e laparoscópica sobre a taxa de prenhez de ovelhas inseminadas em tempo-fixo. Ciência Animal Brasileira, 8, 127–133.Google Scholar
  32. Reefman, N., Kaszàs, F.B., Weschsler, B., and Gygax, L., 2009. Ear and tail postures indicators of emotional valence in sheep. Applied Animal Behaviour Science, 118, 199–207.CrossRefGoogle Scholar
  33. Sayre, B.L., and Lewis, G.S., 1997. Fertility and ovum fertilization rate after laparoscopic or transcervical intrauterine artificial insemination of oxytocin-treated ewes. Theriogenology, 48, 267–275.CrossRefGoogle Scholar
  34. Turner, A.I., Rivalland, E.T.A., Clarke, I.J., and Tilbrook, A.J., 2010. Stressor specificity of sex differences in hypothalamo-pituitary-adrenal axis activity: cortisol responses to exercise, endotoxin, wetting, and isolation/restraint stress in gonadectomized male and female sheep. Endocrinology, 151, 4324–4331.CrossRefGoogle Scholar
  35. Witter, F., Rocco, L., and Johnson, T., 1992. A randomized trial of prostaglandin E2 in a controlled-release vaginal pessary for cervical ripening at term. American Journal of Obstetrics & Gynecology, 166, 830–834.CrossRefGoogle Scholar
  36. Wulster-Radcliffe, M.C., Costine, B.A., and Lewis, G. S., 1999. Estradiol-17-oxytocin-induced cervical dilation in sheep: application to transcervical embryo transfer. Journal of Animal Science, 77, 2587–2593.CrossRefGoogle Scholar
  37. Wulster-Radcliffe, M.C., and Lewis, G., 2002. Development of a new transcervical artificial insemination method for sheep: effects of a new transcervical artificial insemination catheter and traversing the cervix on semen quality and fertility. Theriogenology, 58, 1361–1371.CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Renata P. B. Carneiro
    • 1
  • Gustavo G. Macedo
    • 2
  • Rafael DeRossi
    • 3
    Email author
  • Eliane V. Costa-e-Silva
    • 1
  • Maria Inês L. Souza
    • 4
  1. 1.Department of Veterinary Medicine, Animal Reproduction Laboratory, Faculty of Veterinary MedicineFederal University of Mato Grosso do SulCampo GrandeBrazil
  2. 2.Department of Animal Reproduction, Faculty of Veterinary MedicineFederal University of UberlândiaUberlândiaBrazil
  3. 3.Department of Veterinary Medicine, Surgery and Anesthesiology, Faculty of Veterinary MedicineFederal University of Mato Grosso do SulCampo GrandeBrazil
  4. 4.Institute of Biosciences (InBio)Federal University of Mato Grosso do SulCampo GrandeBrazil

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