Short communication: detection and molecular characterization of hepatitis E virus in domestic animals of São Tomé and Príncipe
As in most of the African continent, the status of hepatitis E virus (HEV) infection in domestic animals in São Tomé and Príncipe, an archipelago off the western equatorial coast of Central Africa, is also completely unknown. In the present study, we investigated the presence of HEV among domestic animals in São Tomé and Príncipe. A total of 93 stool samples from different animal species (goat, cow, pig, chicken, duck, and monkey) were tested for HEV RNA using two real-time RT-PCR assays, followed by a nested RT-PCR assay for sequencing and phylogenetic analysis. A total of six samples (1 cow stool and 5 pig stools) were found to be positive for HEV RNA of which one pig stool was positive by broad spectrum nested RT-PCR. Phylogenetic analysis showed that the retrieved sequence clustered within HEV subgenotype 3f, similar to zoonotic strains of European countries and posing interesting questions on past introduction of European HEV into São Tomé and Príncipe archipelago. This is the first report describing the presence and molecular characterization of HEV in São Tomé and Príncipe.
KeywordsHepatitis E virus São Tomé e Príncipe Domestic animals Zoonoses
We acknowledge the support of the Ministry of Health of São Tomé e Príncipe and the representatives of the non-governmental organization Instituto Marquês de Valle Flor, Dr. Edgar Neves, Dr. António Lima (in STP), and Dr. Ahmed Zaky (in Lisbon) for guaranteeing the local logistic as well as the Calouste Gulbenkian Foundation in Lisbon, Portugal, to contribute to the project consumables.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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