Advertisement

Tropical Animal Health and Production

, Volume 51, Issue 2, pp 355–361 | Cite as

The effects of the conjunctival Brucella vaccine on some biochemical parameters in sheep

  • Gülay ÇiftciEmail author
  • Özkan Yiğit
  • Alper Çiftci
Regular Articles
  • 106 Downloads

Abstract

The genus Brucella causes significant economic losses due to infertility, abortion, stillbirth or weak calves, and neonatal mortality in livestock. Brucellosis is still a zoonosis of public health importance worldwide. In the past, vaccination was administered subcutaneously and nowadays, the conjunctival vaccine is administered. There is no definite information about the changes of the biochemical parameters and antibody response after conjunctival vaccination. In this study, the investigation of the changes in the levels of some biochemical parameters due to the conjunctival vaccination for brucellosis was aimed. Thirty sheep were used as an animal material. The vaccine was done single dose against Brucella melitensis and the blood was drawled from Vena jugularis during 4 months. Antibody levels were determined by serum tube agglutination test. Alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), glucose, total protein, and albumin levels were measured using commercial autoanalyzer in sera. The antibody titers (p < 0.001) increased significantly at first month compared to the pre-vaccination, but at the second month began to fall. There was no statistically significant changes in glucose, AST, ALT activity after vaccination (p > 0.005). The significant amount of total protein and ALP decreased after vaccination (p < 0.005). LDH levels and total protein levels were significantly increased (p < 0.005). In conclusion, conjunctival vaccine was considered to be used as a safe to protect the sheep from brucellosis and the results of the study may be used to improve the efficiency of brucellosis eradication programs within livestock management.

Keywords

Biochemical parameters Brucella melitensis Conjunctival vaccine Sheep 

Notes

Acknowledgements

Supported by Scientific Research Projects Commission of Ondokuz Mayis University (Project No: PYO.VET.1904.14.002).

Compliance with ethical standards

This work was carried out in accordance with the Ethics Committee Decision of the Ondokuz Mayıs University Local Ethics Committee Decision (2013/44).

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Abboud, M., El Rammouz, R., Lahoud, C. and Antonios S., 2015. Immunological Response of Awassi Sheep to Conjunctival Vaccination against Brucellosis Disease in Mount Lebanon. Middle East Journal of Agriculture Research, 04, 967–974.Google Scholar
  2. Alamian, S., Nejad, R.B., Jalali, H.R., Kalantari, A. and Etemadi, A., 2015. Innocuousness of conjunctival vaccination with Brucella melitensis strain Rev.1 in pregnant Iranian fat-tailed ewes. Veterinary Science Development, 5, 112–114.CrossRefGoogle Scholar
  3. Arslan, S.H., Hassan, M.M., Mohammed, H.A., Al-Hussary, N.A. and Al-Obaidi, Q.T., 2011. Remove from marked Records Changes in some biochemical parameters accompanied with brucellosis in sheep. Iraqi Journal of Veterinary Sciences, 25(2), 107–110. Google Scholar
  4. Bain, P.J., 2003. Liver. In: Duncan and Prasse’s Veterinary Laboratory Medicine: Clinical Pathology, Editors: Latimer, K.S., Mahaffey, E.A. and Prasse, K.W., 4th ed. Ames, IA: Iowa State Press, 193–214.Google Scholar
  5. Benkirane, A., Idrissi, A.H., Doumbia, A. and de Balogh, K., 2014. Innocuity and immune response to Brucella melitensis Rev.1 vaccine in camels (Camelus dromedarius). Open Veterinary Journal, 4(2), 96–102.Google Scholar
  6. Chand, P., Chhabra, R. and Nagra, J., 2015. Vaccination of adult animals with a reduced dose of Brucella abortus S19 vaccine to control brucellosis on dairy farms in endemic areas of India. Tropical Animal Health and Production, 47(1), 29–35.CrossRefGoogle Scholar
  7. Da Costa Martins, R., Gamazo, C., Sánchez-Martínez, M., Barberán, M., Peñuelas, I. and Irache, J.M., 2012. Conjunctival vaccination against Brucella ovis in mice with mannosylated nanoparticles. Journal of Controlled Release, 162(3), 553–560.CrossRefGoogle Scholar
  8. El-Azab, M.F.A., 2015. Evaluation of serum enzyme activities and protein fractions in Brucella-infected cows. Turkish Journal of Veterinary&Animal Science, 39, 480–484.Google Scholar
  9. El-Boshy, M., Abbas, H., El-Khodery, S. and Osman, S., 2009. Cytokine response and clinicopathological findings in Brucella infected camels (Camelus dromedarius). Veterinarni Medicina, 541, 25–32.CrossRefGoogle Scholar
  10. European Commission Health and Consumers Directorate, 2009. Working Document on Eradication of Bovine, Sheep and Goats Brucellosis in the EU accepted by the “Bovine” and “Sheep and Goats” Brucellosis subgroups of the Task Force on monitoring animal disease eradication. Veterinary Control Programmes, 31.Google Scholar
  11. Godfroid, J., Cloeckaert, A., Liautard, J.P., Kohler, S., Fretin, D., Walravens, K., Garin-Bastuji, B. and Letesson, J.J., 2005. From the discovery of the Malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. Veterinary Research, 36(3), 313–326.CrossRefGoogle Scholar
  12. Gul, S.T., Khan, A., Ahmad, M. and Hussain, I., 2013. Seroprevalence of brucellosis and associated hemato-biochemical changes in Pakistani horses. Pakistan Journal of Agricultural Sciences, 50(4), 745–750.Google Scholar
  13. Hamada, D.M., Mohamed, A.H., Mabrouk, A., Emad, M. and Ah, M.E., 2013. Seroprevalence of abortion causing agents in Egyptian sheep and goat breeds and their effects on the animal’s performance. Journal of Agricultural Sciences, 5, 92–101.Google Scholar
  14. Hinkle, D.E., Wiersma, W. and Jurs, S.G., 2003. Applied Statistics for the Behavioral Sciences, 5th ed. Boston, Mass: Houghton Mifflin.Google Scholar
  15. Kardjadj, M. and Benmahdi, M., 2014. The “effects” of brucella Rev-1 conjunctival vaccination of sheep and goats on human and animal brucellosis in high plateaus area, Algeria. Frontiers in Immunology, Conference Abstract: The First International Congress of Immunology and Molecular Immunopathology (CIMIP2014).Google Scholar
  16. Kumar, A.V., Srikanth, N.R., Naresh, G. and Vidya, B., 2015. Assessment and comparison of serum biochemical parameters of Brucella infected and healthy ewes. Journal of Livestock Science, 6, 100–103.Google Scholar
  17. Kushwaha, N., Rajora, V.S., Mohan, A., Singh, J.L. and Shukla, S.K., 2014. Assessment of Haemato-biochemical Parameters and Therapeutics on Brucella Infected Cattle. Journal of Microbiology Experimentation, 1(2), 12.CrossRefGoogle Scholar
  18. Mailybayeva, A.,Yespembetov, B.,Ryskeldinova, S.,Zinina, N.,Sansyzbay, A.,Renukaradhya, GJ.,Petrovsky, N. and Tabynov, K., 2017. Improved influenza viral vector based Brucella abortus vaccine induces robust B and T-cell responses and protection against Brucella melitensis infection in pregnant sheep and goats. PLoS One, 12(10), e0186484.CrossRefGoogle Scholar
  19. Minas, A., 2006. Control and eradication of brucellosis in small ruminants. Small Ruminant Research, 62(1–2), 101–107.CrossRefGoogle Scholar
  20. Namrata, S., Rajkumar, P., Prakriti, S. and Neelima, P., 2016. Sero-prevalence of Brucellosis and comparison of serum biochemical parameters between infected and healthy goats. International Journal of Agriculture Sciences, 8(3), 988–990.Google Scholar
  21. Nath, R., Das, S., Sarma, S. and Devi, M., 2014. Comparison of blood profiles between healthy and Brucella affected cattle. Veterinary World, 7, 668–670.CrossRefGoogle Scholar
  22. Nicoletti, P., 2010. Brucellosis: Past, Present and Future. Contributions, Section of Medical Science. Prilozi, 31(1), 21–32.Google Scholar
  23. Office International des Epizooties (OIE), 2009. Manual of Standards for Diagnostic tests and Vaccines. Third edition, Paris, France. Caprine and ovine Brucellosis chapter (2.7.2), Bovine Brucellosis, Chapter (2.4.3).Google Scholar
  24. Petris, C.K., Golomb, M. and Philipps, T.E., 2007. Bacterial transcytosis across conjunctival M cells. Immunology and Microbiology, 48, 2172–2177.Google Scholar
  25. Poester, F.P., Nielsen, K., Samartino, L.E. and Yu, W.L., 2010. Diagnosis of Brucellosis. The Open Veterinary Science Journal, 4, 46–60.CrossRefGoogle Scholar
  26. Pulendran, B. and Ahmed, R., 2011. Immunological mechanisms of vaccination. Nature Immunology, 12, 509–517.CrossRefGoogle Scholar
  27. Stockham, S.L. and Scott, M.A., 2008. Fundamentals of Veterinary Clinical Pathology. 2nd ed. Ames, IA, USA: Blackwell Publishing, 639–674.Google Scholar
  28. Stournara, A., Minas, A., Bourtzi-Chatzopoulou, E., Stack, J., Koptopoulos, G., Petridou, E. and Sarris, K., 2007. Assessment of serological response of young and adult sheep to conjunctival vaccination with Rev-1 vaccine by fluorescence polarization assay (FPA) and other serological tests for B. melitensis. Veterinary Microbiology, 119(1), 53–64.CrossRefGoogle Scholar
  29. Ward, D., Jackson, R., Karomatullo, H., Khakimov, T., Kurbonov, K., Amirbekov, M., Stack, J., El-Idrissi, A. and Heuer, C., 2012. Brucellosis control in Tajikistan using Rev 1 vaccine: change in seroprevalence in small ruminants from 2004 to 2009. Veterinary Record, 170(4), 100.CrossRefGoogle Scholar
  30. Webber, M., Krishnan, A., Thomas, N.G. and Cheung, B.M.Y., 2010. Association between serum alkaline phosphatase and C-reactive protein in the United States National Health and Nutrition Examination Survey 2005-2006. Clinical Chemistry and Laboratory Medicine, 48, 167–173.Google Scholar
  31. Wernery, U.., Gyuranecz, M., Kinne, J., Raghavan, R.,Syriac, G., Johnson, B., Kreizinger, Z., Dénes, B., Felde, O., Magyar, T., Jose, S.H., Raja, S., John, J. and Wernery, R., 2017. Laboratory Investigations after Eye Drop Immunisation of Dromedaries with Live Attenuated Brucella melitensis Rev 1 Vaccine. Journal of Camel Practice and Research, 24(1), 9–14.Google Scholar

Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Biochemistry, Faculty of Veterinary MedicineUniversity of Ondokuz MayisSamsunTurkey
  2. 2.Department of Microbiology, Faculty of Veterinary MedicineUniversity of Ondokuz MayisSamsunTurkey

Personalised recommendations