Influence of Nigella sativa seeds, Rosmarinus officinalis leaves and their combination on growth performance, immune response and rumen metabolism in Dorper lambs

  • Kifah Jumaah Odhaib
  • Kazeem Dauda Adeyemi
  • Muideen Adewale Ahmed
  • Muhammad Faseleh Jahromi
  • Shokri Jusoh
  • Anjas Asmara Samsudin
  • Abdul Razak Alimon
  • Halimatun Yaakub
  • Awis Qurni Sazili
Regular Articles

Abstract

The objective of this study was to determine the effects of dietary supplementation of Nigella sativa L. seeds, Rosmarinus officinalis L. leaves and their combination on rumen metabolism, nutrient intake and digestibility, growth performance, immune response and blood metabolites in Dorper lambs. Twenty-four entire male Dorper lambs (18.68 ± 0.6 kg, 4–5 months old) were randomly assigned to a concentrate mixture containing on a dry matter basis either, no supplement (control, T1), 1% R. officinalis leaves (T2), 1% N. sativa seeds (T3) or 1% R. officinalis leaves +1% N. sativa seeds (T4). The lambs had ad libitum access to urea-treated rice straw (UTRS) and were raised for 90 days. Supplemented lambs had greater (P < 0.05) intake of DM and UTRS than the control lambs. The T4 lambs had lower (P < 0.05) nutrient digestibility than those fed other treatments. Total and daily weight gain was greater (P < 0.05) in T2 lambs than those fed other diets. The T3 and T4 lambs had greater (P < 0.05) ruminal pH than the T1 and T2 lambs. Supplemented lambs had lower (P < 0.05) ruminal total volatile fatty acids, acetate, propionate, NH3-N and C18:0 than the control lambs. The T4 lambs had lower (P < 0.05) population of Fibrobacter succinogenes, Ruminococcus albus, methanogens and total protozoa compared with those fed other diets. Supplemented lambs had lower (P < 0.05) neutrophils, basophils and serum urea and greater (P < 0.05) serum IgA and IgG compared with the control lambs. The current results emphasised the variation in the efficacy of medicinal plants in ruminant nutrition.

Keywords

Basophil Fibrobacter succinogenes IgA IgG Methanogens Neutrophils Ruminococcus albus 

Notes

Compliance with ethical standards

This study was conducted following the guidelines of the Research Policy of Universiti Putra Malaysia on Animal welfare and ethics. The experimental protocol was approved by the Universiti Putra Malaysia Animal use and care committee. The care of the experimental goats was in accordance to Malaysian standards.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Kifah Jumaah Odhaib
    • 1
    • 2
  • Kazeem Dauda Adeyemi
    • 1
    • 3
  • Muideen Adewale Ahmed
    • 1
  • Muhammad Faseleh Jahromi
    • 2
  • Shokri Jusoh
    • 1
  • Anjas Asmara Samsudin
    • 1
  • Abdul Razak Alimon
    • 1
  • Halimatun Yaakub
    • 1
  • Awis Qurni Sazili
    • 1
    • 4
    • 5
  1. 1.Department of Animal Science, Faculty of AgricultureUniversiti Putra MalaysiaUPM SerdangMalaysia
  2. 2.Department of Physiology, College of Veterinary MedicineUniversity of BasrahBasraIraq
  3. 3.Department of Animal ProductionUniversity of IlorinIlorinNigeria
  4. 4.Laboratory of Sustainable Animal Production and Biodiversity, Institute of Tropical Agriculture and Food SecurityUniversiti Putra MalaysiaUPM SerdangMalaysia
  5. 5.Halal Products Research InstituteUniversiti Putra MalaysiaUPM SerdangMalaysia

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