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Tropical Animal Health and Production

, Volume 50, Issue 1, pp 91–96 | Cite as

Brucellosis in migratory sheep flock from Maharashtra, India

  • Chhaya P. Sonekar
  • Satyajit Kale
  • Smita Bhoyar
  • Neha Paliwal
  • S. V. Shinde
  • S. P. Awandkar
  • Wiqar Khan
  • S. P. Chaudhari
  • N. V. KurkureEmail author
Regular Articles
  • 158 Downloads

Abstract

Brucellosis is a zoonotic disease worldwide distributed and having the economic as well as public health importance. The prevalence of brucellosis among sheep flock having history of abortions was studied. A total of 229 samples comprising of 157 blood and 72 clinical samples (vaginal swabs) were collected from 157 animals. Clinical samples were processed for the isolation of Brucella melitensis. Serum samples (n = 157) were tested by Rose Bengal plate test (RBPT) and i-ELISA. A total of 68 (43.31%) and 104 (66.24%) samples were positive by RBPT and ELISA, respectively. Brucella isolates (n = 2) were recovered from clinical samples. Both isolates demonstrated amplification for bcsp 31 and IS711 genes. On AMOS PCR, both the isolates amplified at 731 bp, i.e., belongs to B. melitensis species. The incidence of B. melitensis in a migratory flock warns the thorough testing and culling of Brucella-infected sheep from the flock on a continuous basis; otherwise, such incidence will be routine and poor farmers will be at a loss.

Keywords

Sheep Brucella melitensis Seroprevalence Isolation 

Notes

Acknowledgements

The financial help for NAE on “Centre for Zoonoses” by the Indian Council of Agricultural Research (10(14)/2014 EP&HS) to Dr. S. P. Chaudhari is duly acknowledged.

Compliance with ethical standards

Conflict of interest statement

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Chhaya P. Sonekar
    • 1
  • Satyajit Kale
    • 1
  • Smita Bhoyar
    • 1
  • Neha Paliwal
    • 1
  • S. V. Shinde
    • 1
  • S. P. Awandkar
    • 1
  • Wiqar Khan
    • 1
  • S. P. Chaudhari
    • 1
  • N. V. Kurkure
    • 1
    Email author
  1. 1.NAE Project on “Center for Zoonoses”, Nagpur Veterinary CollegeMAFSUNagpurIndia

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