Brucellosis in migratory sheep flock from Maharashtra, India
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Brucellosis is a zoonotic disease worldwide distributed and having the economic as well as public health importance. The prevalence of brucellosis among sheep flock having history of abortions was studied. A total of 229 samples comprising of 157 blood and 72 clinical samples (vaginal swabs) were collected from 157 animals. Clinical samples were processed for the isolation of Brucella melitensis. Serum samples (n = 157) were tested by Rose Bengal plate test (RBPT) and i-ELISA. A total of 68 (43.31%) and 104 (66.24%) samples were positive by RBPT and ELISA, respectively. Brucella isolates (n = 2) were recovered from clinical samples. Both isolates demonstrated amplification for bcsp 31 and IS711 genes. On AMOS PCR, both the isolates amplified at 731 bp, i.e., belongs to B. melitensis species. The incidence of B. melitensis in a migratory flock warns the thorough testing and culling of Brucella-infected sheep from the flock on a continuous basis; otherwise, such incidence will be routine and poor farmers will be at a loss.
KeywordsSheep Brucella melitensis Seroprevalence Isolation
The financial help for NAE on “Centre for Zoonoses” by the Indian Council of Agricultural Research (10(14)/2014 EP&HS) to Dr. S. P. Chaudhari is duly acknowledged.
Compliance with ethical standards
Conflict of interest statement
The authors declare that they have no conflict of interest.
- Abou-Eisha, A.M., 2000. Brucellosis in camels and its relation to public health. Assuit Veterinary Medical Journal, 44(87), 54–64.Google Scholar
- Alton, G.G., 1990. Brucella melitensis. Animal brucellosis, K. Nielsen and J.R. Duncan (Eds). CRC Press, Boca Raton, Florida, 383–410.Google Scholar
- Alton, G.G., Jones, L.M., Angus, R.D. and Verger, J.M. 1988. Techniques for the Brucellosis Laboratory. Institut National de la Recherché Agronomique, Paris.Google Scholar
- Centers for Disease Control and Prevention [CDC]. Brucellosis (Brucella melitensis, abortus, suis, and canis). CDC; 2005. Available at: http://www.cdc.gov/ncidod/dbmd/diseaseinfo/brucellosis_t.htm. Accessed 4 Jun 2007.
- Henault, S., Calvez, D., Thiebaud, M., Boulicre, M., and Garin-Bastuji, B., 2000. Validation of a nested-PCR based on the IS6501/711 sequence for the detection of Brucella in animal samples. Proceedings of the Brucellosis International Research Conference 9I including the 53rd Brucellosis Research Conference, September 7-9; Nimes, France. Paris: Institut National de la Recherché Agronomique.Google Scholar
- Lemu, D., Mamo, H., Deressa, A. and Pal M.,2014. A study on seroprevalence of brucellosis in goats and sheep in East Shewa, Ethiopia. Ethiopian International Journal of Multidisciplinary Research, 1(4), 14–18.Google Scholar
- McGiven J.A., Tucker, J.D., Perrett, L.L., Stack, J.A., Brew, S.D. and MacMillan, A.P., 2003. Validation of FPA and c-ELISA for the detection of antibodies to Brucella abortus in cattle sera and comparison to SAT, CFT and i-ELISA, Journal of Immunological Methods, 278 (1–2) 171–178.CrossRefGoogle Scholar
- OIE, 2000. Manual of Standards for Diagnostic Tests and Vaccines. 4th Edition, Office International des Epizootics, Paris.Google Scholar
- Pathak, A.D., Dubal, Z.B., Doijad, S., Raorane, A., Rodrigues S, Naik, R., Naik-Gaonkar, S., Naik, R. and Barbuddhe, S.B., 2014. Human brucellosis among pyrexia of unknown origin cases and occupationally exposed individuals in Goa Region, India, Emerging Health Threats Journal, 7, 23846.CrossRefGoogle Scholar
- See, W., Edwards, W.H., Dauwalter, S., Almendra, C., Kardos, M.D., Lowell, J.L., Wallen, R., Cain, S.L., Holben, W.E. and Luikart, G., 2012. Yersinia enterocolitica an unlikely cause of positive brucellosis tests in greater Yellowstone ecosystem bison (Bison bison), Journal of Wildlife Diseases, 48 (3), 537–541.CrossRefGoogle Scholar
- Suryawanshi, S.N., Tembhurne, P.A., Gohain, S. and Ingle, V.C., 2014. Prevalence of Brucella antibodies in Sheep and Goats in Maharashtra. Indian Research Journal of Extension Education, 14 (4), (Special issue on Veterinary Research & Extension) 75–77.Google Scholar
- Sutariya, M.B., Roy, A., Kumar, P. and Patel, P.K., 2014. Seroprevalence of brucellosis in sheep and goats of Gujarat. The Indian Journal of Small Ruminants, 20(1), 128–130Google Scholar