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Multidrug-resistant Salmonellae isolated in Japanese quails reared in Abeokuta, Nigeria

Abstract

Salmonellosis is a major bacterial disease causing huge economic losses in the poultry industry worldwide. This study was carried out to determine the period prevalence and antimicrobial susceptibility of Salmonella enterica in Japanese quails in Abeokuta, Nigeria. Four hundred cloacal swabs of quail birds were collected from 4 locations within Abeokuta. Salmonella was isolated from the samples using conventional methods for selective isolation of Salmonella and biochemical identification. Isolates were confirmed by polymerase chain reaction assays for the amplification and detection of Salmonella-associated virulence genes (invA and stn) using specific primers. Antimicrobial susceptibility testing was done using the Kirby-Bauer disk diffusion method. In all, Salmonella was isolated from 14 (3.5%) cloacal swabs. All 14 isolates possessed invA and stn genes. The Salmonella isolates showed resistance to tetracycline (100%), doxycycline (100%), ampicillin (100%), sulphamethoxazole (92.9%), nalidixic acid (85.8%), ceftazidime (78.6%), neomycin (64.3%), streptomycin (50%) and gentamycin (28.6%) but all the isolates were susceptible to ciprofloxacin. The isolates were resistant to at least three antimicrobials indicating multidrug resistance. The results concluded that Japanese quails harbour multidrug-resistant Salmonella which could be transmitted to humans through consumption of contaminated food or by direct and indirect contact with the carrier birds. Antimicrobial resistance could be due to overdependence on antimicrobials. Ciprofloxacin could be considered in the treatment of zoonotic Salmonellosis in humans.

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Acknowledgements

The authors sincerely thank Dr. M. I. Takeet for guidance and Dr. A. I. Famuyide for technical assistance.

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Correspondence to Ezekiel O. Omoshaba.

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The authors declare that they have no conflict of interest.

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Omoshaba, E.O., Olufemi, F.O., Ojo, O.E. et al. Multidrug-resistant Salmonellae isolated in Japanese quails reared in Abeokuta, Nigeria. Trop Anim Health Prod 49, 1455–1460 (2017). https://doi.org/10.1007/s11250-017-1347-z

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Keywords

  • Inv a
  • Multidrug resistance
  • Quail
  • Salmonella
  • Stn