A cross-sectional study was conducted in three counties (Damxung, Maizhokunggar and Yadong) in Tibet in April and May 2015. A total of 1,523 yaks owned by 181 herders were randomly selected and blood sampled. Sera were tested using the rose bengal test (RBT) and a competitive immune-enzymatic assay (C-ELISA) and the test results interpreted in parallel. The individual yak prevalence was 2.8% (95% CI 2.0–3.7) with a herd prevalence of 18.2% (95% CI 12.9–24.6). At the individual level, two predictor variables, age and production system, were significantly associated with seropositivity by a binary logistic regression analysis. The odds of Brucella infection were significantly higher in older Yaks (3–5 years old, OR = 4.51; 95% CI 1.53–19.29; ≥6 years old, OR = 3.89; 95% CI 1.23–17.21) compared to those of younger yaks (≤2 years old). The odds of seropositivity for yaks managed under an agro-pastoral production system were 2.9 (95% CI 1.48–5.86) times higher compared to those managed under a pastoral production system. At the herd level, an association between the infection with Brucella and a history of abortions in the herd was observed (OR = 4.98, 95% CI 1.48–16.62). Surprisingly, vaccination was not associated with a lower level of infection (p = 0.49 and p = 0.99 for individual and herd level data, respectively). The results of the survey indicate that bovine brucellosis is endemic among the yak population in the plateau region of China, and the risk factors identified in the study should be considered in the epidemiology of the disease and when developing control programs for the disease.
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Alhaji, N.B., Wungak, Y.S., and Bertu, W.J., 2016. Serological survey of bovine brucellosis in Fulani nomadic cattle breeds (Bos indicus) of North-central Nigeria: potential risk factors and zoonotic implications, Acta Tropica, 153, 28–35
Anonymous, 1985. Comments of preventing Brucellosis in Tibet, Tibetan Journal of Science and Technology, 4, 47–49. (In Chinese)
Anonymous, 2013. Accelerating development of agriculture and animal husbandry in China, Process of Agricutural Products, 6, 16–19. (In Chinese)
Aune, K., Rhyan, J.C., Russell, R., Roffe, T.J., and Corso, B., 2012. Environmental persistence of Brucella abortus in the Greater Yellowstone Area, The Journal of Wildlife Management, 76, 253–261
Baghiyan, G., and Shirvanyan, A.Y., 2016. Evaluation of the effectiveness of experimental vaccination of large and small cattle against brucellosis in the Republic of Armenia, Annals of Agrarian Science, 14, 283–287
Bandyopadhyay, S., Sasmal, D., Dutta, T.K., Ghosh, M.K., Sarkar, M., Sasmal, N.K., and Bhattacharya, M., 2009. Seroprevalence of brucellosis in yaks (Poephagus grunniens) in India and evaluation of protective immunity to S19 vaccine, Tropical Animal Health and Production, 41, 587–592
Boukary, A.R., Saegerman, C., Abatih, E., Fretin, D., Bada, R.A., De Deken, R., Harouna, H.A., Yenikoye, A., and Thys, E., 2013. Seroprevalence and potential risk factors for Brucella spp. infection in traditional cattle, sheep and goats reared in urban, periurban and rural areas of niger, PloS one, 8, e83175
Bureau of Veterinary, M.O.A., P.R.China, 2009. Animal Health in China, (China Agriculture Press, Beijing)
Díaz, A.E., 2013. Epidemiology of brucellosis in domestic animals caused by Brucella melitensis, Brucella suis and Brucella abortus, Revue Scientifique et Technique, 32, 43–51, 53–60
Gall, D., and Nielsen, K., 2004. Serological diagnosis of bovine brucellosis: a review of test performance and cost comparison, Revue Scientifique et Technique, 23, 989–1002
Gao, J., Zhang, K., Shahzad, M., Zhang, D., Han, Z., and Li, J., 2013. Seroprevalence of Brucella infection in yaks (Bos grunniens ) in Tibet, China, Cattle practice, 21, 109–111
Gerald, W., Han, J., and Long, R., 2003. The yak, (FAO Regional Office for Asia and the Pacific, Bangkok)
Godfroid, J., Cloeckaert, A., Liautard, J.P., Kohler, S., Fretin, D., Walravens, K., Garin-Bastuji, B., and Letesson, J.J., 2005. From the discovery of the Malta fever’s agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis, Veterinary Research, 36, 313–326
Han, L., Fu, C.X., Jin, X.J., Yuan, L., Li, G., Fang, X., Ji, W.H., and Wang, Y.T., 2009. Change of antibody after vaccinated by S2 among dairy, Chinese Journal of Veterinary Medicine, 45, 82–83. (In Chinese)
He, S.Z., Song, R.D., Niu, Y.J., Game, J.Y., Zhang, Q.L., and Li, J.K., 2015. Serological detection against brucellosis of yak in Yushu area, China Cattle Science, 41, 94–95. (In Chinese)
Jackson, D.S., Nydam, D.V., and Altier, C., 2014. Prevalence and risk factors for brucellosis in domestic yak Bos grunniens and their herders in a transhumant pastoralist system of Dolpo, Nepal, Preventive Veterinary Medicine, 113, 47–58
Jergefa, T., Kelay, B., Bekana, M., Teshale, S., Gustafson, H., and Kindahl, H., 2009. Epidemiological study of bovine brucellosis in three agro-ecological areas of central Oromiya, Ethiopia, Revue Scientifique Et Technique, 28, 933–943
Ma, J.Y., Bao, Y.H., Xia, C. Y, and Song, T. Z, 2014. Situation, problems and developmental strategies of cultivation industry of Tibetan yak, China Cattle Science, 40, 74–74. (In Chinese)
Matope, G., Bhebhe, E., Muma, J.B., Oloya, J., Madekurozwa, R.L., Lund, A., and Skjerve, E., 2011. Seroprevalence of brucellosis and its associated risk factors in cattle from smallholder dairy farms in Zimbabwe, Tropical Animal Health and Production, 43, 975–982
Nyima, T., Luo, X.G., Yu, S.X., and Judson, G., 2005. A survey of the mineral status of livestock in the Tibet Autonomous Region of China, (Australian Centre for International Agricultural Research, Canberra)
OIE, 2009. Bovine brucellosis. In Manuals of Diagnostic Tests and Vaccines for Terrestrial animals (Chapter.2.4.3).2009. http://www.oie.int. Accessed 15 Sep 2016
Praud, A., Durán-Ferrer, M., Fretin, D., Jaÿ, M., O'Connor, M., Stournara, A., Tittarelli, M., Travassos Dias, I., and Garin-Bastuji, B., 2016. Evaluation of three competitive ELISAs and a fluorescence polarisation assay for the diagnosis of bovine brucellosis, The Veterinary Journal, 216, 38–44
R Development Core Team, 2013. R:A language and environment for statistical computing.Vienna, Austria:R Foundation for Statistical Computing.2013. http://www.r-project.org. Accessed 2 Mar 2016
Sergeant, E., Epitools epidemiological calculators. AusVet Animal Health Services and Australian Biosecurity Cooperative Research Centre for Emerging Infectious Disease.2015. http://epitools.ausvet.com.au. Accessed 5 Feb 2015
Sing, T., Sander, O., Beerenwinkel, N., and Lengauer, T., 2005. ROCR: visualizing classifier performance in R, Bioinformatics, 21, 3940–3941
Sun, M.J., Di, D.D., Li, Y., Zhang, Z.C., Yan, H., Tian, L.L., Jing, Z.G., Li, J.P., Jiang, H., and Fan, W.X., 2016. Genotyping of Brucella melitensis and Brucella abortus strains currently circulating in Xinjiang, China, Infection, Genetics and Evolution, 44, 522–529
Wang, Y., Wang, J., Li, S., and Qin, D., 2014. Vulnerability of the Tibetan pastoral systems to climate and global change, Ecology and Society, 19, 8
Wu, G.Z., Zhong, Z., Pingcuo, Z., Bai, J., La, Z., Laqiong, Deji, Dawa, Dawa, C., Laba, Z., Dazhen, W., and Dawa, C., 2011. Epidemiological survey of brucellosis on cattle and sheep, Tibetan Journal of Animal Husbandry and Veterinary, 2, 15–17. (In Chinese)
Yuan, L.G., Shi, Q., and Pu, J.W., 2014. Investigation of brucellosis infection of ruminants on natural pastures, Chinese Journal of Veterinary Medicine, 50, 84–85. (In Chinese)
ZhanDui, Ruan, S.L., Gongsang, Q., and Cui, B.Y., 2008. Epidemiological analysis of brucellosis in Naqu and Qamdo, Tibet in 2006, Disease Surveillance 23, 285–286. (In Chinese)
Zuo, H.L., Fu, P.S., and Du, J., 2009. Countermeasure and change of environment and climate in Tibet, Tibetan Journal of Science and Technology, 6, 55–58. (In Chinese)
We would like to thank the John Allwright Fellowship from ACIAR (Australian Centre for International Agricultural Research) and Murdoch University for sponsoring this study as part of a PhD studentship. The Tibet Livestock Research Institute and Veterinary stations in the three sampled counties are acknowledged for their field assistances during sample collection. Also, we acknowledge the staff of the China Animal Health and Epidemiology Center for their laboratory assistance.
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Zeng, J., Duoji, C., Yuan, Z. et al. Seroprevalence and risk factors for bovine brucellosis in domestic yaks (Bos grunniens) in Tibet, China. Trop Anim Health Prod 49, 1339–1344 (2017). https://doi.org/10.1007/s11250-017-1331-7
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