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Tropical Animal Health and Production

, Volume 49, Issue 5, pp 1077–1080 | Cite as

Detection and molecular characterization of porcine kobuvirus in piglets in 2009–2013 in northern Thailand

  • Watchaporn Chuchaona
  • Pattara KhamrinEmail author
  • Arpaporn Yodmeeklin
  • Aphisek Kongkaew
  • Ratchaya Vachirachewin
  • Kattareeya Kumthip
  • Hiroshi Ushijima
  • Niwat Maneekarn
Short Communications

Abstract

A total of 636 fecal samples collected from piglets with and without diarrhea during 2009 to 2013 were tested for porcine kobuvirus by RT-PCR. From a total of 528 fecal samples collected from piglets with diarrhea and 108 from healthy controls, 505 (95.6%) and 104 (96.3%) were positive for porcine kobuvirus, respectively. The detection rates of porcine kobuvirus were remarkable equally high in both diarrheic and healthy piglets. Phylogenetic analysis revealed that porcine kobuvirus strains detected in both symptomatic and asymptomatic piglets were genetically closely related to each other and also to other porcine kobuviruses reported worldwide. It was interesting to point out that one of the porcine kobuvirus strains isolated from piglet in our study was similar to a porcine-like bovine kobuvirus reference strain isolated previously in South Korea. This finding provided the evidence to support the interspecies transmission of kobuviruses between cattle and swine.

Keywords

Porcine kobuvirus Diarrhea Piglet Epidemiology Thailand 

Notes

Acknowledgements

This study was funded by the grants from the Faculty of Medicine, Chiang Mai University (grant number 83/2555), Center of Excellence (Emerging and Re-emerging Diarrheal Viruses Research Center), Chiang Mai University (grant number ST6392(11)/1089), Thailand.

Compliance with ethical standards

Statement of animal rights

All applications during the study were performed according to animal rights, and the manuscript does not contain clinical studies or patient data.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Watchaporn Chuchaona
    • 1
  • Pattara Khamrin
    • 1
    Email author
  • Arpaporn Yodmeeklin
    • 1
  • Aphisek Kongkaew
    • 2
  • Ratchaya Vachirachewin
    • 3
  • Kattareeya Kumthip
    • 1
  • Hiroshi Ushijima
    • 4
  • Niwat Maneekarn
    • 1
  1. 1.Department of Microbiology, Faculty of MedicineChiang Mai UniversityChiang MaiThailand
  2. 2.Animal House Unit, Faculty of MedicineChiang Mai UniversityChiang MaiThailand
  3. 3.Department of Food Animal Clinic, Faculty of Veterinary MedicineChiang Mai UniversityChiang MaiThailand
  4. 4.Division of Microbiology, Department of Pathology and MicrobiologyNihon University School of MedicineTokyoJapan

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