Tropical Animal Health and Production

, Volume 49, Issue 5, pp 1035–1040 | Cite as

Incidence and knowledge of bovine brucellosis in Kahuro district, Murang’a County, Kenya

  • Janeffer N. NjugunaEmail author
  • Michael M. Gicheru
  • Lucy M. Kamau
  • Peter M. Mbatha
Regular Articles


Brucellosis in cattle is a zoonosis mainly caused by Brucella abortus. In Kenya, the disease is widespread, but its prevalence is largely unknown. The objective of this study was to investigate incidence rates of brucellosis and farmers’ knowledge on the disease in Kahuro district, Murang’a County. In this study, 150 pooled milk samples were collected from 75 milk collection centers and tested. Subsequently, 230 milk samples were collected from farmers in 16 collection centers in Wangu and Mugoiri divisions whose pooled samples gave positive results. Five cow owners in each of the 16 collection centers were interviewed using a questionnaire to assess their knowledge levels. Wangu division had the highest incidence rate 19% with positive samples observed from 14 collection centers. Mugoiri division recorded 3% with two collection centers having positive samples, while Murarandia had none. All respondents with no formal education were unaware of the causative agent of brucellosis. There was a significant difference in incidence between Mugoiri and Wangu divisions (p < 0.05). Knowledge levels were high in the young and educated farmers compared to the old and uneducated. Frequent screening for brucellosis to identify infected animals should be initiated thus prevent transmission to other animals and humans.


Brucellosis Zoonosis Transmission and incidence 



The authors wish to acknowledge the director of Veterinary Services and head of Central Veterinary Laboratories Kabete (CVL) for granting permission to carry out the study in their laboratories. We thank all the staff working at the CVL for their invaluable support during data collection. We acknowledge the farmers who agreed to participate in this study, without whom this work would not have been realized.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical clearance

Approval of the study was obtained from Graduate School Kenyatta University. To ensure confidentiality, code names instead of farmer’s names were used. The farmers were informed on the study purpose prior to commencement of the research through questionnaire preliminaries.


  1. Bayemi, P. H., Mah, G. D., Ndamukong, K.,. Nsongka, V. M., Leinyuy, I.,Unger, H., Ndoumbe, N. M., Webb, E. C., Achukwi, M. D., Hakoue, F. and Luogbou, N. D. 2015. Bovine brucellosis in cattle production systems in the western highlands of Cameroon. International Journal of Animal Biology, 1 (2), 38–44Google Scholar
  2. Deshmukh, A., Hagen, F., Sharabasi, O. A., Abraham, M., Wilson, G., Doiphode, S. and Meis, J. M. 2015. In vitro antimicrobial zusceptibility testing of human Brucella melitensis isolates from Qatar between 2014–2015. BMC Microbiology, 15 (121), 1–5Google Scholar
  3. Dirar, B. G., Nasinyama, G. W. and Gelalcha, B.D., 2015. Seroprevalence and risk factors for brucellosis in cattle in selected districts of Jimma zone, Ethiopia. Tropical Animal Health and Production, 47(8), 1615–1619CrossRefPubMedGoogle Scholar
  4. District veterinary office (DVO), Murang’a North, 2015. Annual reports on diseases infecting cattle in Kahuro District, 5th October 2015Google Scholar
  5. Ducrotoy, M. J., Bertu, W. J., Ocholi, R. A., Gusi, A. M., Bryssinckx, W., Welburn, S. and Moriyon, I. 2014. Brucellosis as an emerging threat in developing economies: lessons from Nigeria. PLoS Neglected Tropical Diseases, 8(7), 1–18CrossRefGoogle Scholar
  6. Ducrotoy, M. J., Ammary, K., Lbacha, A. H., Zouagui, Z., Mick, V., Prevost, L., Bryssinckx, W., Weburn, S. C. and Benkirane, A. 2015. Narrative overview of animal and human brucellosis in Morocco: intensification of livestock production as a driver for emergence? Infectious Diseases of Poverty, 4 (57), 1–21Google Scholar
  7. Hegazy, Y., Elmonir, W., Abdel-Hamid, N. H. and Elbauomy, E. M. 2015. Seroprevalence and “Knowledge, Attitudes and Practices” (KAPs) survey of endemic ovine brucellosis in Egypt. Acta Veterinaria Scandinavica, 58(1), 1–7CrossRefGoogle Scholar
  8. Lindahl, E., Sattorov, N., Boqvist, S. and Magnusson, U. 2015. A Study of knowledge, attitudes and practices relating to brucellosis among small-scale dairy farmers in an urban and peri-urban area of Tajikistan. Plos One, 10(2), 1–10CrossRefGoogle Scholar
  9. McDermott, J., Grace, D. and Zinsstag, J. 2013. Economics of brucellosis impact and control in low-income countries. Revue Scientifique et Technique de l'OIE, 32, 249–261CrossRefPubMedGoogle Scholar
  10. Mohamand, N., Gunaseelan, L., Sukumar, B. and Porteen, K. 2014. Milk Ring Test for spot identification of Brucella abortus infection in single cow herds. Journal of Advanced Veterinary and Animal Research, 1(2), 70–72CrossRefGoogle Scholar
  11. Njeru, J., Wareth, G., Melzer, F., Henning, K., Pletz, M. W., Heller, R. and Neubauer, H. 2016. Systematic review of brucellosis in Kenya: disease frequency in humans and animals and risk factors for human infection. BMC Public Health, 16(1), 1–15Google Scholar
  12. Roushan, M. R. H., and Ebrahimpour, S. 2015. Human brucellosis: an overview. Caspian Journal of Internal Medicine, 6(1), 46–47Google Scholar
  13. Rubach, M. P., Halliday, J. E., Cleaveland, S., Crump, J. A. 2013. Brucellosis in low-income and middle-income countries. Current Opinion in Infectious Diseases, 26(5), 404–412CrossRefPubMedPubMedCentralGoogle Scholar
  14. Ryan T. P., 2013. Sample size determination and power, 1st edition. John Wiley & Sons, IncCrossRefGoogle Scholar
  15. Ogola, E., Thumbi, S., Osoro, E., Osoro, E., Munyua, P., Omulo, S., Mbatha, P., Ochieng, L., Marwanga, D., Njeru, I., Mbaabu, M., Wanyoike, S. and Njenga, K. (2014). Sero-prevalence of brucellosis in humans and their animals: a linked cross-sectional study in two selected counties in Kenya. Online Journal of Public Health Informatics, 6(1), 1CrossRefGoogle Scholar
  16. Singh, B. B., Dhand, N. K. and Gill, J. P. S., 2015. Economic losses occurring due to brucellosis in Indian livestock populations. Preventive Veterinary Medicine, 119(3–4), 211–215CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  • Janeffer N. Njuguna
    • 1
    Email author
  • Michael M. Gicheru
    • 1
  • Lucy M. Kamau
    • 1
  • Peter M. Mbatha
    • 2
  1. 1.Department of Zoological SciencesKenyatta UniversityNairobiKenya
  2. 2.Ministry of Agriculture, Livestock and Fisheries, Central Veterinary LaboratoriesNairobiKenya

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