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Sheep fed with banana leaf hay reduce ruminal protozoa population

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Abstract

A ciliate protozoa suppression can reduce methane production increasing the energy efficiency utilization by ruminants. The physicochemical characteristics of rumen fluid and the profile of the rumen protozoa populations were evaluated for sheep fed banana leaf hay in replacement of the Cynodon dactylon cv. vaqueiro hay. A total of 30 male sheep were raised in intensive system during 15 days of adaptation and 63 days of experimental period. The animals were distributed in a completely randomized design that included six replicates of five treatments with replacement levels (0, 25, 50, 75, and 100%) of the grass vaquero for the banana leaf hay. Samples of fluid were collected directly from the rumen with sterile catheters. Color, odor, viscosity, and the methylene blue reduction potential (MBRP) were evaluated and pH estimated using a digital potentiometer. After decimal dilutions, counts of genus protozoa were performed in Sedgewick Rafter chambers. The averages of pH, MBRP, color, odor, and viscosity were not influenced by the inclusion of the banana leaf hay. However, the total number of protozoa and Entodinium spp. population significantly decreased at 75 and 100% inclusions of banana leaf hay as roughage.

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Acknowledgements

This work was supported by the National Council for Scientific and Technological Development (CNPq), the Coordination for the Improvement of Higher Education Personnel (CAPES), the Foundation for the State of Minas Gerais Research (FAPEMIG), and the Dean of Research the Federal University of Minas Gerais.

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Author notes

  1. Eduardo Robson Duarte

    Present address: Institute of Agricultural Sciences, Universidade Federal de Minas Gerais, Montes Claros, MG, 39404-547, Brazil

Authors and Affiliations

  1. Departamento de Zootecnia, Universidade Estadual de Montes Claros (UNIMONTES), Reinaldo Viana Street, 2630, Bico da Pedra Neighbourhood, Janaúba, MG, 39440-000, Brazil

    Cláudio Eduardo Silva Freitas, Dorismar David Alves & Franklin Delano dos Santos Soares

  2. Instituto de Ciências Agrárias, Montes Claros, Universidade Federal de Minas Gerais (UFMG), Universitária Av., 1000, Universitário Neighbourhood, Montes Claros, MG, 39400-006, Brazil

    Eduardo Robson Duarte

  3. Departamento de Zoologia, Universidade Federal de Juiz de Fora, Juiz de Fora, MG, 36036-900, Brazil

    Isabel Martinele, Marta D’Agosto & Franciane Cedrola

  4. Industrial Biotechnology Program, Universidade Estadual de Montes Claros (UNIMONTES), Montes Claros, MG, 39401-089, Brazil

    Angélica Alves de Moura Freitas

  5. Department of Civil and Environmental Engineering, Utah State University, Logan, UT, USA

    Makenzi Beltran

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  1. Cláudio Eduardo Silva Freitas
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Correspondence to Eduardo Robson Duarte.

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The authors declare that they have no conflict of interest.

Ethical approval

All procedures were in accord with protocol number 05/10 approved in the Ethic Committee in the Animal Experimentation of the Universidade Estadual de Montes Claros, Brazil.

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Freitas, C.E.S., Duarte, E.R., Alves, D.D. et al. Sheep fed with banana leaf hay reduce ruminal protozoa population. Trop Anim Health Prod 49, 807–812 (2017). https://doi.org/10.1007/s11250-017-1265-0

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