Tropical Animal Health and Production

, Volume 48, Issue 7, pp 1435–1442 | Cite as

Effect of replacing marine fish meal with catfish (Pangasius hypophthalmus) by-product protein hydrolyzate on the growth performance and diarrhoea incidence in weaned piglets

  • Nguyen Thi ThuyEmail author
  • Nguyen Cong Ha
Regular Articles


The present study consists of two experiments. In experiment 1, a total of 120 weaned piglets (Yorkshire × Landrace) (7.3 ± 1.9 kg) were allocated to five treatments, and four replications (pens) with six piglets/pen. In experiment 2, 40 male pigs (23.0 ± 2.2 kg) were allocated to five treatments and eight replications (individual pens). In both experiments, the control diet contained fish meal (FM) as the sole protein supplement (CPH0), while the experimental diets consisted of four different diets in which crude protein (CP) from FM in CPH0 was replaced by the CP from catfish (Pangasius hypophthalmus) by-product protein hydrolyzate (CPH) at four different levels: 100 % (CPH100), 75 % (CPH75), 50 % (CPH50) and 25 % (CPH25). The results in experiment 1 showed that the highest average daily gain (ADG) over the 5-week period after weaning was recorded for piglets on CPH100 (307 g/day), and the lowest for piglets fed CPH0 (287 g/day) (P < 0.01). Feed conversion ratio (FCR) was lower in CPH100 (1.43 kg feed/kg gain) than in CPH0 (1.51 kg feed/kg gain) (P < 0.01). The piglets fed CPH100 were less affected by diarrhoea (6.55 %) than piglets fed the control diet CPH0 (17.3 %) after weaning, and faecal scores were also lower. In experiment 2, ADG was lowest in CPH0, and the cost/gain in pigs fed CPH100 was lowest in both weaning and growing pigs. In conclusion, it is possible to replace up to 100 % of the FM by CPH in diets for weaning and growing pigs, resulting in improved ADG and FCR, lower feed cost/gain as well as reduced diarrhoea incidence and improved faecal score.


Catfish By-products Diarrhoea Growing pigs Protein hydrolyzate Weaned piglets 



This research is funded by the Vietnam National Foundation for Science and Technology Development (Nafosted) under grant number 106-NN.05-2013.68. Sincere thanks to Vinh Khanh pig farm in An Giang Province for allowing us to carry out the experiments. Thanks are also due to Dr. Brian Ogle for the language revision.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Research funder

The National Foundation for Science and Technology Development (Nafosted) of Vietnam.

Grant code number: 106-NN.05- 2013.68.


  1. AOAC., 2000. Official Methods of Analysis (17th ed.). Association of Official Analytical Chemists, Inc., Arlington, Virginia, USAGoogle Scholar
  2. Batista, I., Ramos, C., Coutinho, J., Bandarra, N.M., Nunes, M.L., 2010. Characterization of protein hydrolysates and lipids obtained from black scabbardfish (Aphanopus carbo) by-products and antioxidative activity of the hydrolysates produced, Process Biochemistry, 45, 18–24.CrossRefGoogle Scholar
  3. Cupere, D.F., Deprez, P., Demeulenaere, D., Muylle, E., 1992. Evaluation of the effects of 3 probiotics on experimental Escherichia Coli enterotoxaemia in weaned piglets, Journal of Veterinary Medicine. B, 39, 277–284.Google Scholar
  4. Folador, J.F., Karr-Lilienthal, L.K., Parsons, C.M., Bauer, L.L., Utterback, P.L., Schasteen, C.S., Bechtel, P.J., Fahey, Jr G.C., 2006. Fish meal, fish components, and fish protein hydrolysates as potential ingredients in pet foods, Journal of Animal Sciences, 84, 2752–2765.Google Scholar
  5. Gilbert, E.R., Wong, E.A., Webb, Jr K.E., 2008. Board-invited review: Peptide absorption and utilization: implications for animal nutrition and health, Journal of Animal Sciences, 86, 2135–2155.Google Scholar
  6. Hien, D.M., Ha, N.C., Thuy, N.T., Cuong, T.H., 2015. The hydrolysis ability of red meat by-product protein from Catfish in case of high fat content using enzyme Bromelain, International Proceedings of Food Ingredients Asia Conference 2015, Bitec, Bangkok, Thailand, 10–11 Sep 2015, 105–110.Google Scholar
  7. Minh, N.P., 2014. Utilization of Pangasius Hypophthalmus by-product to produce protein hydrolysate using alcalase enzyme, Journal Of Harmonized Research in Applied Sciences, 2 (3), 250–256.Google Scholar
  8. Noblet, J., Le Bellego, L., Van Milgen, J., Dubois, S., 2001. Effects of reduced dietary protein level and fat addition on heat production and nitrogen and energy balance in growing pigs, Animal Research, 50 (3), 227–238.CrossRefGoogle Scholar
  9. Nørgaard, J.V., Blaabjerg, K., Poulsen, H.D., 2012. Salmon protein hydrolysate as a protein source in feed for young pigs, Animal Feed Science and Technology, 177, 124–129.CrossRefGoogle Scholar
  10. Parisini, P., Scicipioni, P., 1989. Effects of peptid in a proteolysate in piglet nutrition, Zootecnica e Nutriz-ione-animale, 15, 637–644.Google Scholar
  11. Thuy, N.T., Loc, N.T., Lindberg, J.E., Ogle, B., 2007. Survey of the production, processing and nutritive value of Catfish by-product meals in the Mekong Delta of Vietnam, Livestock Research for Rural Development, 19 (9), 124 Scholar
  12. Thuy, N.T., Lindberg, J.E., Ogle, B., 2010. Digestibility and Nitrogen balance of diets that include marine fish meal, catfish (Pangasius hypophthalmus) by-product meal and silage, and processing waste water in growing pigs, Asian-Australasian Journal of Animal Sciences, 23 (7), 924–930.CrossRefGoogle Scholar
  13. Thuy, N.T., Lindberg, J.E., Ogle, B., 2011. Effects of replacing fish meal with catfish (Pangasius hypophthalmus) processing waste water on the performance of growing pigs, Tropical Animal Health and Production, 43, 425–430.CrossRefPubMedGoogle Scholar
  14. Tucker, J.L., Naranjo, V.D., Bidner, T.D., Southern, L.L., 2011. Effect of salmon protein hydrolysate and spray-dried plasma protein on growth performance of weanling pigs, Journal of Animal Sciences, 89, 1466–1473.Google Scholar
  15. Vente-Spreeuwenberg, A.M., Verdonk, J.M.A.J., Koninkx, J.F.J.G., Beynen, A.C., Verstegen, M.W.A., 2004. Dietary protein hydrolysates vs the intact proteins does not enhance mucosal integrity and growth performance in weaned piglets, Livestock Production Science, 5, 151–164.CrossRefGoogle Scholar
  16. Kang-Lyung Woo., 2001. Amino acid analysis protocols, Edited by Catherine Cooper, Nicolle Packer and Keith Williams, Humana Press, Totowa, New Jersey, 141–167.Google Scholar
  17. Wu, G., 1998. Intestinal mucosal amino acid catabolism, Journal of Nutrition, 128, 1249–1252.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  1. 1.Department of Animal Science, College of Agriculture and Applied BiologyCan Tho UniversityCan Tho CityVietnam
  2. 2.Department of Food Technology, College of Agriculture and Applied BiologyCan Tho UniversityCan Tho CityVietnam

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