Incidence of mastitis and activity of milk neutrophils in Tharparkar cows reared under semi-arid conditions
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Rearing of indigenous Tharparkar (TP) cows (native of arid Thar deserts) under high humid conditions (>75 % humidity) has increased the incidence of mammary infections in them. A study was undertaken to see the number, activity, and expression of milk neutrophils isolated from healthy and mastitic cows. There was a significant (P < 0.05) influx in milk somatic cell counts (SCC) and neutrophils in sub-clinical and clinical mastitis cows. No change was observed in the phagocytic activity (PA) of milk neutrophils between healthy and sub-clinical mastitis (SCM) cows, but these activities decreased significantly (P < 0.05) in clinical cases. Chemotactic activity showed a significant difference between all the groups. Lactose varied significantly (P < 0.05) between healthy, sub-clinical, and clinical mastitis (CM) cows. Expression of chemokine receptor (CXCR1) was more in mastitis cows and also higher as compared to CXCR2. No change was observed in cluster of differentiation molecule (CD62L) among all the three groups of TP cows. Expression of interleukin (IL-8) and CD11b was low in healthy cows, increased significantly (P < 0.05) in both sub-clinical and mastitis cows. This study indicates that low producing TP cows are also prone to mammary infections when reared under semi-arid conditions.
KeywordsTharparkar Semi-arid Mastitis Milk Neutrophils Activity Expression
This research was supported by the Department of Biotechnology (Grant Number: BT/PR8003/AAQ/1/544/2013 dated September 7, 2014), Ministry of Science and Technology, Government of India. The first author is also thankful to the Government of Syria for granting a study leave to carry out his post graduate work.
Compliance with ethical standards
Statement of animal rights
All animal experimental procedures were approved by the Animal Ethics Committee of the National Dairy Research Institute, Karnal, Haryana, India.
Conflict of interest
The authors declare that they have no conflict of interest.
- Dang, A.K., Prasad, S., De, K., Pal, S., Mukherjee, J., Sandeep, I.V.R., Mutoni, G., Pathan, M. M., Jamwal, M., Kapila, S., Kapila, R., Kaur, H., Dixit, S., Mohanty, A.K., Prakash, B.S., 2012. Effect of supplementation of vitamin E, copper and zinc on the in vitro phagocytic activity and lymphocyte proliferation index of peripartum Sahiwal (Bos indicus) cows. Journal of Animal Physiology and Animal Nutrition, 97, 315–321.CrossRefPubMedGoogle Scholar
- Della Libera, A.A.M.P., de Souza, F.N., Batista, C. F., Santos, B.P., de Azevedo, L. F. F., Ramos Sanchez, E. M., AraújoDiniz, S., Silva, M.X., Haddad, J.P., Blagitz, M.G., 2015. Effects of bovine leukemia virus infection on milk neutrophil function and the milk lymphocyte profile. Veterinary Research, 46, 2.CrossRefPubMedPubMedCentralGoogle Scholar
- Jones, S.A., Wolf, M., Qin, S., Mackay, C.R., Baggiolini, M., 1996. Different functions for the interleukin 8 receptors (IL-8R) of human neutrophil leukocytes: NADPH oxidase and phospholipase D are activated through IL-8R1 but not IL-8R2. Proceedings of the National Academy of Sciences, 93, 6682- 6686.CrossRefGoogle Scholar
- Le, Marechal., Thiery., Vautor., Le, Loir., 2011. Mastitis impact on technological properties of milk and quality of milk products: A review. Dairy Science and Technology, 91,247-282.Google Scholar
- Santana, M.L., Pereira, R.J., Bignardi, A.B., Vercesi Filho, A.E., Menendez-Buxadera, A., El Faro, L., 2015. Detrimental effect of selection for milk yield on genetic tolerance to heat stress in purebred Zebu cattle: Genetic parameters and trends. Journal of Dairy Science, 98, 9035 – 9043.CrossRefPubMedGoogle Scholar
- Taneja, V.K., 1999. Dairy breeds and selection. https://www.ilri.org/InfoServ/Webpub/fulldocs/SmHDairy/chap5.html.
- Verbeke, J., Boulougouris, X., Rogiers, C., Burvenich, C., Peelman, L., Spiegeleer, B.D. Vliegher, S.D., 2015.Reactive oxygen species generation by bovine blood neutrophils with different CXCR1 (IL8RA) genotype following Interleukin-8 incubation. BMC Veterinary Research, 11,104.CrossRefPubMedPubMedCentralGoogle Scholar
- Watanabe, A., Yagi, Y., Shiono, H., Yokomizo, Y., Inumaru, S., 2008. Effects of intramammary infusions of interleukin-8 on milk protein composition and induction of acute-phase protein in cows during mammary involution. Canadian Journal of Veterinary Research, 72, 291-6.PubMedPubMedCentralGoogle Scholar