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Tropical Animal Health and Production

, Volume 48, Issue 6, pp 1291–1295 | Cite as

Incidence of mastitis and activity of milk neutrophils in Tharparkar cows reared under semi-arid conditions

  • Mohanned Alhussien
  • P. Manjari
  • Seid Mohammed
  • Aasif Ahmad Sheikh
  • Srinu Reddi
  • Satpal Dixit
  • Ajay K. Dang
Short Communications

Abstract

Rearing of indigenous Tharparkar (TP) cows (native of arid Thar deserts) under high humid conditions (>75 % humidity) has increased the incidence of mammary infections in them. A study was undertaken to see the number, activity, and expression of milk neutrophils isolated from healthy and mastitic cows. There was a significant (P < 0.05) influx in milk somatic cell counts (SCC) and neutrophils in sub-clinical and clinical mastitis cows. No change was observed in the phagocytic activity (PA) of milk neutrophils between healthy and sub-clinical mastitis (SCM) cows, but these activities decreased significantly (P < 0.05) in clinical cases. Chemotactic activity showed a significant difference between all the groups. Lactose varied significantly (P < 0.05) between healthy, sub-clinical, and clinical mastitis (CM) cows. Expression of chemokine receptor (CXCR1) was more in mastitis cows and also higher as compared to CXCR2. No change was observed in cluster of differentiation molecule (CD62L) among all the three groups of TP cows. Expression of interleukin (IL-8) and CD11b was low in healthy cows, increased significantly (P < 0.05) in both sub-clinical and mastitis cows. This study indicates that low producing TP cows are also prone to mammary infections when reared under semi-arid conditions.

Keywords

Tharparkar Semi-arid Mastitis Milk Neutrophils Activity Expression 

Notes

Acknowledgments

This research was supported by the Department of Biotechnology (Grant Number: BT/PR8003/AAQ/1/544/2013 dated September 7, 2014), Ministry of Science and Technology, Government of India. The first author is also thankful to the Government of Syria for granting a study leave to carry out his post graduate work.

Compliance with ethical standards

Statement of animal rights

All animal experimental procedures were approved by the Animal Ethics Committee of the National Dairy Research Institute, Karnal, Haryana, India.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Mohanned Alhussien
    • 1
  • P. Manjari
    • 2
  • Seid Mohammed
    • 2
  • Aasif Ahmad Sheikh
    • 2
  • Srinu Reddi
    • 2
  • Satpal Dixit
    • 3
  • Ajay K. Dang
    • 4
  1. 1.Animal Production DivisionAleppo UniversityAleppoSyria
  2. 2.Lactation and Immuno-Physiology LaboratoryICAR-National Dairy Research InstituteKarnalIndia
  3. 3.Principal Scientist, National Bureau of Animal Genetic ResourcesKarnalIndia
  4. 4.Principal Scientist, Lactation and Immuno-Physiology LaboratoryICAR-National Dairy Research InstituteKarnalIndia

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